Date published: 2025-9-6

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CaMKKα 抗体 (F-2): sc-17827

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说明书
  • CaMKK alpha 抗体 F-2 是小鼠单克隆 IgG2a κ,CaMKKα抗体, 在6篇文献中引用,规格为200 µg/ml
  • 免疫rat物种的CaMKKα的氨基酸1-73
  • CaMKK alpha 抗体 (F-2) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse 和rat 来源的 CaMKKα
  • 抗CaMKK alpha抗体(F-2)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取CaMKKα (F-2): sc-17827免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG2a BP-HRPm-IgGκ BP-HRP 是 CaMKKα 抗体 (F-2) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与CaMKKα 抗体 (F-2) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

CaMKKα抗体(F-2)是一种小鼠单克隆IgG2a kappa轻链抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)、免疫组织化学和酶联免疫吸附测定(ELISA)检测小鼠和大鼠来源的CaMKKα蛋白。抗CaMKKα抗体(F-2)有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶(HRP)、藻红蛋白(PE)、异硫氰酸荧光素(FITC)和多种Alexa Fluor®结合物。CaMKKα蛋白在细胞信号传导通路中起着至关重要的作用,是钙/钙调蛋白依赖性蛋白激酶的关键调节因子,而钙/钙调蛋白依赖性蛋白激酶对于肌肉收缩、神经递质释放和基因表达等各种生理过程至关重要。具体而言,CaMKK负责磷酸化并激活CaMKI,从而影响下游信号级联。这种激活在T细胞中尤为重要,因为T细胞受体CD3复合体的刺激会导致CaMKI活性显著增加,从而影响免疫反应。了解CaMKKα的功能及其在这些信号通路中的相互作用对于阐明其在健康和疾病中的作用至关重要,因此CaMKKα(F-2)抗体成为研究人员研究钙信号及其在各种生物学环境中的意义的重要工具。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

CaMKKα 抗体 (F-2) 参考文献:

  1. Arl4D-EB1相互作用促进中心粒招募EB1和微管生长  |  Okuno, S., et al. 2001. J Biochem. 130: 503-13. PMID: 11574070
  2. Ca2+/钙调蛋白依赖性蛋白激酶α调节骨骼肌葡萄糖摄取,不受AMP激活蛋白激酶和Akt激活的影响。  |  Witczak, CA., et al. 2007. Diabetes. 56: 1403-9. PMID: 17287469
  3. 14-3-3蛋白通过磷酸化依赖性多位点结合直接调节Ca(2+)/钙调蛋白依赖性蛋白激酶α。  |  Ichimura, T., et al. 2008. FEBS Lett. 582: 661-5. PMID: 18242179
  4. CAMKK1在调节间充质干细胞分泌物中的新作用。  |  Dong, F., et al. 2017. Stem Cells Transl Med. 6: 1759-1766. PMID: 28688176
  5. 心脏肌浆网磷酰班激酶是一种独特的 delta-CaM 激酶同工酶。  |  Baltas, LG., et al. 1995. FEBS Lett. 373: 71-5. PMID: 7589437
  6. 钙离子/钙调蛋白依赖性蛋白激酶级联的特征。钙/钙调蛋白依赖性蛋白激酶激酶的分子克隆和表达。  |  Tokumitsu, H., et al. 1995. J Biol Chem. 270: 19320-4. PMID: 7642608
  7. 钙/钙调蛋白依赖性蛋白激酶II能下调人类T细胞中钙调素和蛋白激酶C介导的细胞因子基因转录途径。  |  Hama, N., et al. 1995. J Exp Med. 181: 1217-22. PMID: 7869038
  8. KN-93 是 CaMK-II(多功能 Ca2+/CaM 激酶)的一种阻抑剂,可诱导 NIH 3T3 细胞的 G1 细胞周期停滞和细胞凋亡。  |  Tombes, RM., et al. 1995. Cell Growth Differ. 6: 1063-70. PMID: 8519682
  9. 过表达 Ca2+/calmodulin 依赖性蛋白激酶 II 可抑制 PC12 细胞的神经元生长。  |  Tashima, K., et al. 1996. J Neurochem. 66: 57-64. PMID: 8522989
  10. Jurkat T 淋巴细胞中 CaM 激酶对 Ca2+/calmodulin 依赖性蛋白激酶(CaM-激酶)IV 的激活。  |  Park, IK. and Soderling, TR. 1995. J Biol Chem. 270: 30464-9. PMID: 8530476
  11. Ca2+/钙调蛋白依赖性蛋白激酶α在大鼠中枢神经系统的分布:免疫组织化学研究。  |  Nakamura, Y., et al. 1996. Neurosci Lett. 204: 61-4. PMID: 8929978
  12. Ca2+/钙调蛋白依赖性蛋白激酶激酶α底物特异性研究。  |  Okuno, S., et al. 1997. J Biochem. 122: 337-43. PMID: 9378711

订购信息

产品名称产品编号规格价格数量收藏夹

CaMKKα 抗体 (F-2)

sc-17827
200 µg/ml
RMB2377.00

CaMKKα (F-2): m-IgG Fc BP-HRP 套装

sc-528327
200 µg Ab; 10 µg BP
RMB2662.00

CaMKKα (F-2): m-IgGκ BP-HRP 套装

sc-520673
200 µg Ab, 40 µg BP
RMB2662.00

CaMKKα (F-2): m-IgG2a BP-HRP 套装

sc-547074
200 µg Ab; 10 µg BP
RMB2662.00

CaMKKα 抗体 (F-2) AC

sc-17827 AC
500 µg/ml, 25% agarose
RMB3129.00

CaMKKα 抗体 (F-2) HRP

sc-17827 HRP
200 µg/ml
RMB2377.00

CaMKKα 抗体 (F-2) FITC

sc-17827 FITC
200 µg/ml
RMB2482.00

CaMKKα 抗体 (F-2) PE

sc-17827 PE
200 µg/ml
RMB2580.00

CaMKKα 抗体 (F-2) Alexa Fluor® 488

sc-17827 AF488
200 µg/ml
RMB2685.00

CaMKKα 抗体 (F-2) Alexa Fluor® 546

sc-17827 AF546
200 µg/ml
RMB2685.00

CaMKKα 抗体 (F-2) Alexa Fluor® 594

sc-17827 AF594
200 µg/ml
RMB2685.00

CaMKKα 抗体 (F-2) Alexa Fluor® 647

sc-17827 AF647
200 µg/ml
RMB2685.00

CaMKKα 抗体 (F-2) Alexa Fluor® 680

sc-17827 AF680
200 µg/ml
RMB2685.00

CaMKKα 抗体 (F-2) Alexa Fluor® 790

sc-17827 AF790
200 µg/ml
RMB2685.00

Are there any issues with using sc-17827: CaMKKα (F-2) mouse monoclonal primary antibody on mouse samples?

Asked by: Randy McDonald
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent,we recommend using a directly conjugated primary antibody. Please contact our special orders department for pricing and availability of sc-17827 conjugates.
Answered by: Technical Support
Date published: 2017-03-07

I wanted CaMKKα Anticuerpo (R-73): sc-11370 (https://www.scbt.com/scbt/es/product/camkkalpha-antibody-r-73?requestFrom=search) for human use, and the web page me recommends me this. However, is not intended for humans. Is there another solution?

Asked by: JLLV
Thank you for your question. We have not yet tested this mouse monoclonal, CaMKKα Antibody (F-2): sc-17827, in human samples so we are not sure if it will work or not. We are currently recommending this antibody as an alternative to sc-11370 because we have strong in-house Western blot data, and we are offering a complimentary 10µg sample of this antibody for a limited time. We also offer an alternative antibody that is recommended for detection of human, CaMKKα Antibody (6): sc-136280. (https://www.scbt.com/scbt/product/camkkalpha-antibody-6). Please contact our Technical Service Department if you would like assistance choosing the best product for your research needs.
Answered by: Technical Support
Date published: 2017-01-27
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Rated 4 out of 5 by from nice work in heartthis antibody really nice work in mice heart lizate
Date published: 2018-04-06
Rated 5 out of 5 by from Good WB data with rat HeartI am very glad for using this new antibody,diluted 1:2000 with rat Heart,got the very well bands
Date published: 2017-01-10
Rated 5 out of 5 by from Great monoclonal antibodyGreat monoclonal antibody, strong specific bands in PC-12 whole cell lysate and rat brain tissue extract . -SCBT QC
Date published: 2013-02-15
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CaMKKα 抗体 (F-2) is rated 4.7 out of 5 by 3.
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