Date published: 2025-9-7
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Calretinin Antibody (H-5) - Western Blotting - Image 362930
钙网蛋白抗体 (H-5): sc-365956. 人小脑 (A), 人脑 (B), 人肾上腺 (C) 和大鼠小脑 (D) 组织提取物中钙网蛋白表达的 Western 印迹分析.
钙网蛋白抗体 (H-5): sc-365956. 人小脑 (A), 人脑 (B), 人肾上腺 (C) 和大鼠小脑 (D) 组织提取物中钙网蛋白表达的 Western 印迹分析.

Calretinin 抗体 (H-5): sc-365956

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说明书
  • Calretinin 抗体 H-5 是小鼠单克隆 IgG2b κ，Calretinin抗体, 在17篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于human物种的Calretinin的N-terminus的氨基酸2-27之间
  • Calretinin 抗体 (H-5) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 Calretinin; 也和以下物种反应，包括： 和 equine and porcine
  • 抗Calretinin抗体（H-5）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Calretinin (H-5): sc-365956的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgGκ BP-HRP 是 Calretinin 抗体 (H-5) 在 WB and IHC(P) 应用中的首选二抗检测试剂。该试剂现与Calretinin 抗体 (H-5) 组合成套装提供。 (请参阅下面的订购信息)。其他m-IgGκ BP 偶联产品，请参阅我们的小鼠 IgG 结合蛋白完整列表。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

Calretinin抗体（H-5）是一种IgG2b κ小鼠单克隆Calretinin抗体（也被命名为CALB2抗体、29 KDa Calbindin抗体、CAB29抗体、CAL2抗体、CR抗体或Calbindin 2 29kDa（Calretinin）抗体），可通过WB、IP、IF、IHC（P）和ELISA检测小鼠、大鼠和人类的Calretinin蛋白。Calretinin抗体（H-5）既可作为非共轭抗Calretinin抗体形式存在，也可作为多种共轭抗Calretinin抗体形式存在，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®共轭物。钙结合蛋白D28K和钙网蛋白（也被命名为CR或29 kDa钙结合蛋白）是两种密切相关的细胞内钙结合蛋白，属于肌钙蛋白C超家族。钙网蛋白最初是从鸡视网膜中分离出来的，与人类钙结合蛋白D28K有58%的相同残基。钙网蛋白在大脑中表达，并且在具有精确放电时间的听觉神经元中尤为丰富。伏隔核中含有钙网蛋白的神经元都具有核凹陷。钙网蛋白免疫反应的突触在棘突、树突和胞体上形成不对称和对称的突触特化。对称的突触特化具有中等大小的棘状神经元，并与其他钙网蛋白免疫反应的胞体接触。钙网蛋白被广泛用作间皮瘤的免疫细胞化学标记物。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Calretinin 抗体 (H-5) 参考文献:

  1. 人脑钙结合蛋白 calretinin 的结构及其在细菌中的表达。  |  Parmentier, M. and Lefort, A. 1991. Eur J Biochem. 196: 79-85. PMID: 2001709
  2. 人类睾丸在整个胎儿期的钙网蛋白免疫活性  |  Altobelli, GG., et al. 2017. J Cell Physiol. 232: 1872-1878. PMID: 27990638
  3. 颗粒细胞卵巢肿瘤中 ER alpha、抑制素 A、钙网素和 Ki67 表达的免疫组化意义。  |  Balan, RA., et al. 2017. Rom J Morphol Embryol. 58: 753-760. PMID: 29250651
  4. 丘脑中线的钙调素神经元调节饥饿诱导的唤醒状态  |  Hua, R., et al. 2018. Curr Biol. 28: 3948-3959.e4. PMID: 30528578
  5. 过表达钙凝蛋白会增强短期突触抑制  |  Bolshakov, AP., et al. 2019. Front Cell Neurosci. 13: 91. PMID: 30930749
  6. 恶性胸膜间皮瘤患者血浆钙网蛋白的决定因素。  |  Lehnert, M., et al. 2020. BMC Res Notes. 13: 359. PMID: 32727552
  7. 与前额叶-海马回路相关的丘脑中线钙凝蛋白和钙宾蛋白结构  |  Viena, TD., et al. 2021. Hippocampus. 31: 770-789. PMID: 33085824
  8. 钙调蛋白：一种主要在神经元中表达的新型钙结合蛋白的基因。  |  Rogers, JH. 1987. J Cell Biol. 105: 1343-53. PMID: 3654755
  9. 石棉相关疾病中遗传因素与血清钙黄绿素水平的关联。  |  Zupanc, C., et al. 2023. Radiol Oncol. 57: 473-486. PMID: 38038422
  10. 光镜和电子显微镜对大鼠脑核中含有 NADPH-二磷酸酶, 钙视蛋白和副发光素的神经元的研究。  |  Hussain, Z., et al. 1996. J Chem Neuroanat. 10: 19-39. PMID: 8703362
  11. 钙网蛋白：间皮瘤的新型免疫细胞化学标记物。  |  Doglioni, C., et al. 1996. Am J Surg Pathol. 20: 1037-46. PMID: 8764740
  12. 大鼠上丘视网膜感受层中钙宾蛋白-28/kD的本体表达时空模式  |  Dreher, B., et al. 1996. J Comp Neurol. 376: 223-40. PMID: 8951639

订购信息

产品名称产品编号规格价格数量收藏夹

Calretinin 抗体 (H-5)

sc-365956
200 µg/ml
RMB2377.00

Calretinin (H-5): m-IgGκ BP-HRP 套装

sc-522442
200 µg Ab, 40 µg BP
RMB2662.00

Calretinin 抗体 (H-5) AC

sc-365956 AC
500 µg/ml, 25% agarose
RMB3129.00

Calretinin 抗体 (H-5) HRP

sc-365956 HRP
200 µg/ml
RMB2377.00

Calretinin 抗体 (H-5) FITC

sc-365956 FITC
200 µg/ml
RMB2482.00

Calretinin 抗体 (H-5) PE

sc-365956 PE
200 µg/ml
RMB2580.00

Calretinin 抗体 (H-5) Alexa Fluor® 488

sc-365956 AF488
200 µg/ml
RMB2685.00

Calretinin 抗体 (H-5) Alexa Fluor® 546

sc-365956 AF546
200 µg/ml
RMB2685.00

Calretinin 抗体 (H-5) Alexa Fluor® 594

sc-365956 AF594
200 µg/ml
RMB2685.00

Calretinin 抗体 (H-5) Alexa Fluor® 647

sc-365956 AF647
200 µg/ml
RMB2685.00

Calretinin 抗体 (H-5) Alexa Fluor® 680

sc-365956 AF680
200 µg/ml
RMB2685.00

Calretinin 抗体 (H-5) Alexa Fluor® 790

sc-365956 AF790
200 µg/ml
RMB2685.00

Calretinin (H-5) 中和勝肽

sc-365956 P
100 µg/0.5 ml
RMB511.00

Does FITC conjugated anti-Calretinin Ab work in flow cytometry?

Asked by: Ramin13
For Protocol compatibility inquiry please contact Technical Service.
Answered by: Tech Sales
Date published: 2021-05-12

We are considering using this conjugated antibody for mouse brain. From which animal species serum should the blocking solution be used? for antibody the host is unclear?

Asked by: cccyildirim
The host is Mouse. Blocking conditions are assay dependent, for more detail please contact Technical Service.
Answered by: Tech Sales
Date published: 2021-04-29

What application is the blocking peptide sc-365956 P appropriate for?

Asked by: jenniferc
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Good signal for Inner Hair cells of the cochleaI received it as a free sample and it worked really well for inner hair cells of the cochlea in a wholemount of mouse inner ear. It also worked to stain Spiral ganlgion neurons cell bodies.
Date published: 2018-08-30
Rated 5 out of 5 by from nice for IHCThis antibody was generously given to me by the trials program. I found that it works well for IHC at a dilution of 1:50 in both whole mount and cross-sections of retinal tissue. It works better in MetOH fixation than in PFA.
Date published: 2018-03-14
Rated 4 out of 5 by from good staining of amacrine cells in human retina!Good staining for PFA-fixed paraffin embedded human retina sections, staineds clearly amacrine cell bodies, but processess were not clear enough.
Date published: 2018-02-15
Rated 5 out of 5 by from Clear staining in mice spinal cordI used 1:100 sample antibody to do IF in frozen slice of mice spinal cord, and got clear staining compatible to those published figures.
Date published: 2017-06-25
Rated 5 out of 5 by from Excellent result in human tissueI tried the sample on human tissue and It worked perfectly. No backround, no nonespecific signal. Higly recomend.
Date published: 2017-05-21
Rated 5 out of 5 by from works on macaque tissuthis antibody recognizes calretinin in paraffin embedded macaque retina tissue when used at a dilution of 1:50 ON
Date published: 2017-01-03
Rated 5 out of 5 by from GreatGreat, strong band in WB in rat brain tissue extract. -SCBT QC
Date published: 2015-04-12
Rated 5 out of 5 by from Excellent immunoperoxidase cytoplasmic stainingExcellent immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human adrenal gland tissue. -SCBT QC
Date published: 2015-03-03
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Calretinin 抗体 (H-5) is rated 4.9 out of 5 by 9.
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