Date published: 2025-9-8
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Calpain 1 Antibody (D-11) - Western Blotting - Image 378670
钙蛋白酶 1 抗体 (D-11): sc-271313. TF-1 (A), RPMI2650 (B) 和 AN3 CA (C) 全细胞裂解液中 Calpain 1 表达的 Western 印迹分析.
钙蛋白酶 1 抗体 (D-11): sc-271313. TF-1 (A), RPMI2650 (B) 和 AN3 CA (C) 全细胞裂解液中 Calpain 1 表达的 Western 印迹分析.

Calpain 1 抗体 (D-11): sc-271313

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说明书
  • Calpain 1 抗体 D-11 是小鼠单克隆 IgG1 κ，Calpain 1抗体, 在15篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于human物种的Calpain 1的N-terminus附近的氨基酸8-33之间
  • Calpain 1 抗体 (D-11) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 Calpain 1
  • 抗Calpain 1抗体（D-11）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Calpain 1 (D-11): sc-271313的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP、 1 BP-HRP">m-IgG1 BP-HRP和m-IgGκ BP-HRP是Calpain 1 Antibody (D-11) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与Calpain 1 Antibody (D-11) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

额外Calpain抗体，包括Calpain 1, Calpain 11, Calpain 13, Calpain 15, Calpain 2, Calpain 3, Calpain 5, Calpain 6, Calpain 7, Calpain 9和Calpain reg

Calpain 1抗体（D-11）是一种IgG1 κ小鼠单克隆Calpain 1抗体（也称为钙依赖性半胱氨酸蛋白酶（Calpain）1抗体，或Calpain蛋白酶1抗体），可通过WB、IP、IF和ELISA检测小鼠、大鼠和人类来源的Calpain 1蛋白。Calpain 1抗体（D-11）既可以作为非偶联抗Calpain 1抗体，也可以作为多种偶联形式的抗Calpain 1抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®共轭物。Calpain 1，也被称为µ-calpain，是一种细胞内钙依赖性蛋白酶，可裂解细胞骨架和膜下蛋白。Calpains是非溶酶体、钙激活的细胞内半胱氨酸蛋白酶。Calpains介导特定的Ca2+依赖性过程，包括细胞融合、有丝分裂和减数分裂。Calpains是由一个小调节亚基和三个大催化亚基中的一个组成的异二聚体，被命名为Calpain 1、Calpain 2和Calpain p94。Calpastatin通过抑制Calpain的蛋白水解活性及其与膜的结合来调节Calpain。Calpastatin存在于两种类型中，组织型和红细胞型，这是由选择性剪接和蛋白水解加工产生的。在衰老的大脑中，Calpain-1与活化的小胶质细胞上的人类白细胞抗原-DR（HLA-DR）共定位。Calpain影响精子发生过程和受精前的事件，如顶体反应。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Calpain 1 抗体 (D-11) 参考文献:

  1. 家族性肥厚型心肌病中发现的肌钙蛋白 I 突变体的钙蛋白酶-1 依赖性降解。  |  Barta, J., et al. 2003. Mol Cell Biochem. 251: 83-8. PMID: 14575308
  2. 钙蛋白酶-1调节中性粒细胞凋亡中的Bax和随后的Smac依赖性caspase-3活化。  |  Altznauer, F., et al. 2004. J Biol Chem. 279: 5947-57. PMID: 14612448
  3. 小鼠精原细胞和精子中钙蛋白酶 1 和钙蛋白酶 2 的表达。  |  Ben-Aharon, I., et al. 2005. Reproduction. 129: 435-42. PMID: 15798018
  4. 红细胞钙蛋白的分子多样性。  |  Takano, E., et al. 1991. Biomed Biochim Acta. 50: 517-21. PMID: 1801717
  5. 钙蛋白酶1可裂解并灭活糖尿病小鼠肠系膜动脉中的前列环素合成酶。  |  Randriamboavonjy, V., et al. 2017. Basic Res Cardiol. 112: 10. PMID: 28013348
  6. 钙蛋白酶-2与钙蛋白酶-1一样，在同一个位点特异性切割结蛋白-2，但效果较差。  |  Wang, J., et al. 2021. Biochem J. 478: 3539-3553. PMID: 34524407
  7. 钙蛋白酶1抑制剂可减轻人源化镰状细胞病模型中的体内血栓形成。  |  Mithila, F., et al. 2022. Thromb Res. 211: 123-126. PMID: 35149397
  8. 人参皂苷Rg1通过钙蛋白酶1途径抑制线粒体活性氧的形成，从而改善慢性间歇性缺氧引起的血管内皮功能障碍。  |  Zhao, F., et al. 2023. J Ginseng Res. 47: 144-154. PMID: 36644390
  9. 钙依赖蛋白酶和特异性抑制剂：钙蛋白酶和钙司他丁。  |  Murachi, T. 1984. Biochem Soc Symp. 49: 149-67. PMID: 6100833
  10. 人类红细胞中钙磷脂的分子多样性。  |  Takano, E., et al. 1993. Arch Biochem Biophys. 303: 349-54. PMID: 8512320
  11. 膜对钙蛋白酶-钙司他丁系统的调控（综述）。  |  Kawasaki, H. and Kawashima, S. 1996. Mol Membr Biol. 13: 217-24. PMID: 9116760
  12. 钙蛋白酶：完整而活跃？  |  Johnson, GV. and Guttmann, RP. 1997. Bioessays. 19: 1011-8. PMID: 9394623

订购信息

产品名称产品编号规格价格数量收藏夹

Calpain 1 抗体 (D-11)

sc-271313
200 µg/ml
RMB2377.00

Calpain 1 (D-11): m-IgG Fc BP-HRP 套装

sc-529162
200 µg Ab; 10 µg BP
RMB2662.00

Calpain 1 (D-11): m-IgGκ BP-HRP 套装

sc-521909
200 µg Ab, 40 µg BP
RMB2662.00

Calpain 1 (D-11): m-IgG1 BP-HRP 套装

sc-543361
200 µg Ab; 20 µg BP
RMB2662.00

Calpain 1 抗体 (D-11) AC

sc-271313 AC
500 µg/ml, 25% agarose
RMB3129.00

Calpain 1 抗体 (D-11) HRP

sc-271313 HRP
200 µg/ml
RMB2377.00

Calpain 1 抗体 (D-11) FITC

sc-271313 FITC
200 µg/ml
RMB2482.00

Calpain 1 抗体 (D-11) PE

sc-271313 PE
200 µg/ml
RMB2580.00

Calpain 1 抗体 (D-11) Alexa Fluor® 488

sc-271313 AF488
200 µg/ml
RMB2685.00

Calpain 1 抗体 (D-11) Alexa Fluor® 546

sc-271313 AF546
200 µg/ml
RMB2685.00

Calpain 1 抗体 (D-11) Alexa Fluor® 594

sc-271313 AF594
200 µg/ml
RMB2685.00

Calpain 1 抗体 (D-11) Alexa Fluor® 647

sc-271313 AF647
200 µg/ml
RMB2685.00

Calpain 1 抗体 (D-11) Alexa Fluor® 680

sc-271313 AF680
200 µg/ml
RMB2685.00

Calpain 1 抗体 (D-11) Alexa Fluor® 790

sc-271313 AF790
200 µg/ml
RMB2685.00

Calpain 1 (D-11) 中和勝肽

sc-271313 P
100 µg/0.5 ml
RMB511.00

Hello Does anyone know against which amino acids the antibody is specifically targeted?

Asked by: MarceloChile
Thank you for your question. This monoclonal antibody is specific for an epitope mapping between amino acids 8-33 near the N-terminus of Calpain 1 of human origin. The exact epitope has not been determined.
Answered by: Technical Support
Date published: 2019-05-25

El anticuerpo reconoce la forma activa o procesada de calpaina 1 por WB? (debería verse una doble banda) Gracias

Asked by: oar2
Gracias por tu pregunta. Según fuentes en línea, Calpaina 1 existe como una forma activa de 78 kDa unida a la membrana y una forma activa intracelular de 75 kDa. Calpaína 1 (D-11): sc-271313 se generó contra un péptido corto que abarca los dos sitios de escisión. Sin embargo, dado que no caracterizamos el sitio de unión exacto de este anticuerpo, no se sabe a qué forma activa se une. Nuestro análisis de WB muestra una banda única, pero como las dos formas activas son similares en peso molecular, es difícil determinar qué forma está detectando el anticuerpo. Es posible que sea necesario realizar estudios adicionales de inmunotinción. Thank you for your question. According to online sources, Calpain 1 exists as a membrane-bound 78 kDa active form and an intracellular 75 kDa active form. Calpain 1 (D-11): sc-271313 was raised against a short peptide spanning the two cleavage sites. However, since we do not characterize the exact binding site of this antibody, it is uncertain which active form it binds to. Our WB analysis shows a single band but since the two active forms are similar in molecular weight, it is hard to determine which form the antibody is detecting. Further immunostaining studies may need to be performed.
Answered by: Technical Support
Date published: 2018-12-31

What application is the blocking peptide sc-271313 P appropriate for?

Asked by: Dr Ninau Qelp
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from D-11Hello Does anyone know against which amino acids the antibody is specifically targeted?
Date published: 2018-04-13
Rated 4 out of 5 by from Clean bandsGood results with human cardiac stem cells (30ug). 1:200 primary antibody dilution. Anti-mouse secondary 1:1000.
Date published: 2018-02-20
Rated 4 out of 5 by from Decent AntibodyGot a sample of this antibody from Santa Cruz. Works well for WB at 1:1000 dilution
Date published: 2017-12-13
Rated 5 out of 5 by from Calpain 1 antibodyThe sample received for trial works very good and specific to target. i would recommend this antibody for use
Date published: 2017-10-23
Rated 3 out of 5 by from Good for WBI got a test sample, and it is work for WB. The MW should be 80kDa, but I found it is lower than 75kDa.
Date published: 2017-09-07
Rated 5 out of 5 by from suitable for WBwestern blot of MCF-7 cell lysate with dilution of 1:5000
Date published: 2017-08-26
Rated 5 out of 5 by from Worked as advertised!I was given a free trial for this antibody and it worked as directed. No issues!
Date published: 2017-07-24
Rated 3 out of 5 by from Robust band seen in WB using KRobust band seen in WB using K-562 whole cell lysate. -SCBT QC
Date published: 2015-01-12
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Calpain 1 抗体 (D-11) is rated 4.2 out of 5 by 9.
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