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Calpain 1/2 抗体 (28F3): sc-58326

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说明书
  • Calpain 1/2 抗体 28F3 是小鼠单克隆 IgG1,Calpain 1/2抗体, 在4篇文献中引用,规格为100 µg/ml
  • 针对全长钙蛋白酶2human来源
  • 推荐用于 human 和 bovine 来源的native and denatured 28 to 30 kDa small subunit of Calpain 1 and Calpain 2 (mu-Calpain and m-Calpain) WB, IP 和 ELISA检测
  • 看 Calpain 1 (D-11): sc-271313,可查看有标记的 Calpain 1/2 抗体,包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • 目前,我们还没有完成Calpain 1/2 抗体 (28F3)的首选二抗检测试剂的鉴定。这项工作正在进行中。
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钙蛋白酶1/2抗体(28F3)是一种小鼠单克隆IgG1抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)和酶联免疫吸附测定(ELISA)检测人源和牛源的钙蛋白酶1/2蛋白。抗钙蛋白酶1/2抗体(28F3)以非结合形式提供。钙蛋白酶是一种细胞内钙依赖性蛋白酶,在细胞骨架重组和肌肉蛋白降解等各种细胞过程中发挥着关键作用,因此钙蛋白酶对于维持细胞完整性和功能至关重要。钙蛋白酶以异二聚体的形式存在,由一个小的调节亚基和三个大的催化亚基之一组成:钙蛋白酶1、钙蛋白酶2和钙蛋白酶p94。值得注意的是,钙蛋白酶的活性受到钙蛋白抑制剂的严格调控,后者可抑制钙蛋白酶的蛋白水解活性及膜结合。钙蛋白抑制剂有两种形式--组织型和红细胞型,这是由可变剪接和蛋白水解加工产生的,凸显了钙蛋白酶调控的复杂性。了解钙蛋白酶的相互作用和调控机制对于阐明钙蛋白酶在健康和疾病中的作用至关重要,特别是在肌肉相关疾病和细胞信号通路中。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Calpain 1/2 抗体 (28F3) 参考文献:

  1. 小鼠精原细胞和精子中钙蛋白酶 1 和钙蛋白酶 2 的表达。  |  Ben-Aharon, I., et al. 2005. Reproduction. 129: 435-42. PMID: 15798018
  2. 子宫内膜癌中钙蛋白酶1、钙蛋白酶2和钙蛋白酶抑制剂的表达免疫组织化学分析。  |  Salehin, D., et al. 2010. Anticancer Res. 30: 2837-43. PMID: 20683020
  3. 钙蛋白酶-1和钙蛋白酶-2:突触可塑性和神经退化的阴阳。  |  Baudry, M. and Bi, X. 2016. Trends Neurosci. 39: 235-245. PMID: 26874794
  4. 钙蛋白酶-1和钙蛋白酶-2在视网膜缺血/再灌注损伤引起的视网膜神经节细胞变性中起着相反的作用。  |  Wang, Y., et al. 2016. Neurobiol Dis. 93: 121-8. PMID: 27185592
  5. 大鼠和小鼠在卡那丁诱导的癫痫活动中钙蛋白酶-1和钙蛋白酶-2的差异激活。  |  Seinfeld, J., et al. 2016. eNeuro. 3: PMID: 27622212
  6. 使用阴离子交换色谱法一步纯化钙蛋白酶-1、钙蛋白酶-2和钙蛋白酶抑制剂。  |  Biswas, AK. and Tandon, S. 2019. Methods Mol Biol. 1915: 3-11. PMID: 30617791
  7. 酪蛋白酶谱分析法用于分析钙蛋白酶-1和钙蛋白酶-2的活性。  |  Biswas, AK. and Tandon, S. 2019. Methods Mol Biol. 1915: 31-38. PMID: 30617793
  8. 钙蛋白酶-2参与了钙蛋白酶-1的失活过程。  |  Shinkai-Ouchi, F., et al. 2020. Biosci Rep. 40: PMID: 33078830
  9. 大脑中的钙蛋白酶-1和钙蛋白酶-2:钙蛋白酶-2在神经元死亡中发挥关键作用的新证据。  |  Wang, Y., et al. 2020. Cells. 9: PMID: 33339205
  10. 钙蛋白酶-2与钙蛋白酶-1一样,在同一个位点特异性切割结蛋白-2,但效果较差。  |  Wang, J., et al. 2021. Biochem J. 478: 3539-3553. PMID: 34524407

订购信息

产品名称产品编号规格价格数量收藏夹

Calpain 1/2 抗体 (28F3)

sc-58326
100 µg/ml
CNY2422.00

For Western Blot, is it recommended to use denatured or non-denatured conditions with Calpain 1/2 (28F3): sc-58326 antibody?

Asked by: AbPolly
Thank you for your question. We recommend this antibody for use in denatured Western Blot conditions. It has not been validated for use in non-denatured conditions. Please contact our Technical Service Department for further details or inquiries.
Answered by: Technical Support
Date published: 2017-03-27
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Rated 5 out of 5 by hufeng from good specificityVery good specificity and sensitivity for WB with lysate of cell lines from human and mouse.
Date published: 2017-06-07
Rated 5 out of 5 by zhangchuang from stablethis antibody has worked well in Western blotting, good results will continue to use.
Date published: 2017-06-01
Rated 5 out of 5 by SM from WB data published with mouse brain capillaryWB data published with mouse brain capillary cell - SCBT Publication Review
Date published: 2015-05-17
Rated 5 out of 5 by TS from Antibody published in siRNA and WB with humanAntibody published in siRNA and WB with human JJ012 tumor cells and primary chondrocytes - SCBT Publication Review
Date published: 2013-05-27
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Calpain 1/2 抗体 (28F3) is rated 5.0 out of 5 by 4.
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