Date published: 2025-9-8

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Calgranulin B 抗体 (MRP 1H9): sc-53187

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说明书
  • Calgranulin B抗体(MRP 1H9)是小鼠单克隆IgG1 κ, 在6篇文献中引用,规格为200 µg/ml
  • 针对重组MRP-14human来源
  • Calgranulin B 抗体 (MRP 1H9) 推荐用于WB, IP, IF, IHC(P) 和 ELISA,检测human 来源的 Calgranulin B
  • 抗Calgranulin B抗体(MRP 1H9)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Calgranulin B (MRP 1H9): sc-53187免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRPm-IgG1 BP-HRP 是 Calgranulin B 抗体 (MRP 1H9) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Calgranulin B 抗体 (MRP 1H9) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

Calgranulin B 抗体(MRP 1H9)是一种小鼠单克隆 IgG1 kappa 轻链抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)、石蜡包埋切片免疫组织化学(IHCP)和酶联免疫吸附测定(ELISA)检测人类 Calgranulin B。抗钙粒蛋白B抗体(MRP 1H9)有非结合型和结合型两种,分别与琼脂糖、辣根过氧化物酶(HRP)、藻红蛋白(PE)、异硫氰酸荧光素(FITC)和多种Alexa Fluor®染料结合。钙谷蛋白B(Calgranulin B)也称为MRP14,属于S100蛋白家族,以EF-hand钙结合基序为特征。钙谷蛋白B在炎症反应中发挥关键作用,并调节细胞过程,包括细胞增殖和分化。钙谷蛋白B在巨噬细胞和上皮细胞中表达,有助于免疫反应并作为信号分子发挥作用。钙离子结合会影响钙谷蛋白B的构象以及与其他细胞成分的相互作用。钙谷蛋白B在炎症部位的发现凸显了其在免疫反应中的作用,以及作为自身免疫性疾病和癌症生物标志物的潜力。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Calgranulin B 抗体 (MRP 1H9) 参考文献:

  1. 大鼠 S100 alpha cDNA 的分离及其 mRNA 在大鼠组织中的分布。  |  Zimmer, DB., et al. 1991. Brain Res Bull. 27: 157-62. PMID: 1742602
  2. 囊性纤维化抗原单克隆抗体。  |  Hayward, C., et al. 1986. J Immunol Methods. 91: 117-22. PMID: 2425000
  3. 追踪囊性纤维化基因的血清蛋白的组织定位和染色体分配。  |  van Heyningen, V., et al. Nature. 315: 513-5. PMID: 2582274
  4. 结肠癌细胞内钙粘蛋白B的抗肿瘤作用。  |  Kim, K., et al. 2016. Oncotarget. 7: 20368-80. PMID: 26933915
  5. 与结肠癌细胞系HCT-116中的钙粘蛋白B相互作用的蛋白质。  |  Myung, JK., et al. 2017. Oncotarget. 8: 6819-6832. PMID: 28036279
  6. 胃肠道癌细胞中钙粘蛋白B相互作用蛋白的鉴定和网络分析。  |  Kim, KH., et al. 2017. PLoS One. 12: e0171232. PMID: 28152021
  7. 两种相关的囊性纤维化相关钙结合蛋白在正常和异常组织中的表达模式。  |  Wilkinson, MM., et al. 1988. J Cell Sci. 91 (Pt 2): 221-30. PMID: 3267695
  8. 在无血清培养基中生长的下丘脑神经元中的副发光素和钙粘蛋白 D28K 的发育和功能研究  |  Pfyffer, GE., et al. 1987. J Neurochem. 49: 442-51. PMID: 3298546
  9. 大鼠睾丸发育过程中 Ca2+ 结合蛋白parvalbumin, 钙结合蛋白 D-28K, S-100 蛋白和钙调素的发育外观。  |  Kägi, U., et al. 1988. Cell Tissue Res. 252: 359-65. PMID: 3383215
  10. 通过直接注射副缬氨酸 cDNA 提高骨骼肌松弛速度  |  Müntener, M., et al. 1995. Proc Natl Acad Sci U S A. 92: 6504-8. PMID: 7604022
  11. 维甲酸调节钙结合蛋白钙宾蛋白-D28K的表达。  |  Wang, YZ. and Christakos, S. 1995. Mol Endocrinol. 9: 1510-21. PMID: 8584029
  12. 羊水中的一种新型钙结合蛋白 CAAF1:其分子克隆和组织分布。  |  Hitomi, J., et al. 1996. J Cell Sci. 109 (Pt 4): 805-15. PMID: 8718672

订购信息

产品名称产品编号规格价格数量收藏夹

Calgranulin B 抗体 (MRP 1H9)

sc-53187
200 µg/ml
RMB2377.00

Calgranulin B (MRP 1H9): m-IgG Fc BP-HRP 套装

sc-526777
200 µg Ab; 10 µg BP
RMB2662.00

Calgranulin B (MRP 1H9): m-IgG1 BP-HRP 套装

sc-532150
200 µg Ab; 20 µg BP
RMB2662.00

Calgranulin B 抗体 (MRP 1H9) AC

sc-53187 AC
500 µg/ml, 25% agarose
RMB3129.00

Calgranulin B 抗体 (MRP 1H9) HRP

sc-53187 HRP
200 µg/ml
RMB2377.00

Calgranulin B 抗体 (MRP 1H9) FITC

sc-53187 FITC
200 µg/ml
RMB2482.00

Calgranulin B 抗体 (MRP 1H9) PE

sc-53187 PE
200 µg/ml
RMB2580.00

Calgranulin B 抗体 (MRP 1H9) Alexa Fluor® 488

sc-53187 AF488
200 µg/ml
RMB2685.00

Calgranulin B 抗体 (MRP 1H9) Alexa Fluor® 546

sc-53187 AF546
200 µg/ml
RMB2685.00

Calgranulin B 抗体 (MRP 1H9) Alexa Fluor® 594

sc-53187 AF594
200 µg/ml
RMB2685.00

Calgranulin B 抗体 (MRP 1H9) Alexa Fluor® 647

sc-53187 AF647
200 µg/ml
RMB2685.00

Calgranulin B 抗体 (MRP 1H9) Alexa Fluor® 680

sc-53187 AF680
200 µg/ml
RMB2685.00

Calgranulin B 抗体 (MRP 1H9) Alexa Fluor® 790

sc-53187 AF790
200 µg/ml
RMB2685.00

Does the antibody recognize the mouse epitope?

Asked by: LABLAB
Thank you for your question. Calgranulin B Antibody (MRP 1H9) is only recommended for detection of Calgranulin B of human origin by WB, IP, IF, IHC(P) and ELISA. Based on our tests with the product we cannot recommend it for use on murine cell or tissue samples. Please refer to Calgranulin B Antibody (47-8D3): sc-58706 for an alternative compatible with murine material.
Answered by: Tech Support Europe
Date published: 2025-05-09

Can Calgranulin B (MRP 1H9): sc-53187 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: cjMara
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, Calgranulin B (MRP 1H9): sc-53187 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from very good antibodyThis antibody can be used to detect mouse brain Calgranulin B in WB.
Date published: 2018-03-08
Rated 5 out of 5 by from Great for IHCThe Calgranulin B Antibody (MRP 1H9): sc-53187 works very well for IHC with formalin fixed, paraffin-embedded tissue sections showing staining pattern localized to the cytoplasm and nucleus. This antibody has also been cited several times for further verification.
Date published: 2017-02-03
Rated 4 out of 5 by from Published IHCP data with human SiHa and CPublished IHCP data with human SiHa and C-33A human cervical squamous carcinoma cell lines - SCBT Publication Review
Date published: 2015-05-16
Rated 4 out of 5 by from Robust signal in WB in DMSO treated HLRobust signal in WB in DMSO treated HL-60 and human PBL whole cell lysates. -SCBT QC
Date published: 2015-02-28
Rated 4 out of 5 by from Produced great immunoperoxidase cytoplasmicProduced great immunoperoxidase cytoplasmic and nuclear staining in formalin fixed, paraffin-embedded human esophagus tissue. -SCBT QC
Date published: 2014-11-22
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Calgranulin B 抗体 (MRP 1H9) is rated 4.4 out of 5 by 5.
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