Date published: 2025-9-8
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C3aR Antibody (D-12) - Western Blotting - Image 82650
C3aR 抗体 (D-12): sc-133172. 经 LPS 处理的 RAW 264. 7 (A) 和 HeLa (B) 全细胞裂解液中 C3aR 表达的 Western 印迹分析.
C3aR 抗体 (D-12): sc-133172. 经 LPS 处理的 RAW 264. 7 (A) 和 HeLa (B) 全细胞裂解液中 C3aR 表达的 Western 印迹分析.

C3aR 抗体 (D-12): sc-133172

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寫評論提問

说明书
  • C3aR 抗体 D-12 是小鼠单克隆 IgG2a κ，C3aR抗体, 在27篇文献中引用，规格为200 µg/ml
  • 针对氨基酸183-482，在C3aR的human起源的C-terminus处进行映射
  • C3aR 抗体 (D-12) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 C3aR
  • 抗C3aR抗体（D-12）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取C3aR (D-12): sc-133172的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP、 2a BP-HRP">m-IgG2a BP-HRP和m-IgGκ BP-HRP是C3aR Antibody (D-12) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与C3aR Antibody (D-12) 打包提供（请参阅下面的订购信息）。

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相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

附加Complement Pathway Protein抗体，包括C3, C3a / α, C3 α, C3aR, C3d, C4, C4 α, C4 β, C5b-9, C5 β, C5L2, C6, C8 γ和C9

C3aR抗体（D-12）是一种IgG2a κ小鼠单克隆C3aR抗体（也称为C3a受体抗体、C3a过敏毒素孤儿G蛋白偶联受体抗体或G蛋白偶联C3a受体抗体），可通过WB、IP、IF和ELISA检测小鼠、大鼠和人类的C3aR蛋白。C3aR抗体（D-12）既可作为非共轭抗C3aR抗体形式，也可作为多种共轭形式的抗C3aR抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®共轭物。补体C3前体包含补体C3β链、补体C3α链、C3a过敏毒素、补体C3bα链、补体C3c片段、补体C3dg片段、补体C3g片段、补体C3d片段和补体C3f片段。C3a、C4a和C5a是补体激活过程中释放的强效过敏毒素。补体激活是造血谱系细胞特有的配体-表面蛋白相互作用系统，有助于消除病原体。C3a和C5a的分泌与哮喘和细菌性脑膜炎等病理生理表现相关。这些蛋白质与各自存在于髓系白细胞表面的G蛋白偶联受体（C3aR、C5aR）结合，会诱导促炎反应，如细胞脱粒、平滑肌收缩、花生四烯酸代谢、细胞因子释放、白细胞活化和细胞趋化性。C3aR在大脑和活化的B淋巴细胞中表达，而C5aR则普遍存在于肝细胞、肺细胞、平滑肌细胞和内皮细胞的表面。激活后，C3aR和C5aR容易发生由GRK介导的快速磷酸化和网格蛋白包被囊泡靶向作用。C5aR利用Ras-Raf-ERK1/2级联反应并与Gi/G16蛋白偶联。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

C3aR 抗体 (D-12) 参考文献:

  1. C3a 活性的调节：人 C3a 受体的内化及其受 C5a 的抑制。  |  Settmacher, B., et al. 1999. J Immunol. 162: 7409-16. PMID: 10358194
  2. C3aR和C5aR受体的配体诱导磷酸化是由 'G蛋白偶联受体激酶 '介导的。  |  Langkabel, P., et al. 1999. Eur J Immunol. 29: 3035-46. PMID: 10508278
  3. C3a无乳毒素受体在哮喘的效应阶段发挥作用。  |  Humbles, AA., et al. 2000. Nature. 406: 998-1001. PMID: 10984054
  4. 人类补体成分 C3：cDNA 编码序列和衍生一级结构。  |  de Bruijn, MH. and Fey, GH. 1985. Proc Natl Acad Sci U S A. 82: 708-12. PMID: 2579379
  5. C3a-C3aR信号通过调节癌相关成纤维细胞促进乳腺癌肺转移。  |  Shu, C., et al. 2020. J Exp Clin Cancer Res. 39: 11. PMID: 31931851
  6. C3aR（C3a过敏反应毒素受体）对易患动脉粥样硬化小鼠动脉粥样硬化的保护作用。  |  Wei, LL., et al. 2020. Arterioscler Thromb Vasc Biol. 40: 2070-2083. PMID: 32762445
  7. 外周血白细胞C3aR mRNA水平与血浆C3a、C3aR、IL-1β浓度以及慢性阻塞性肺病急性加重之间的关联。  |  Li, Z., et al. 2022. Immunobiology. 227: 152164. PMID: 34923262
  8. IL-1R/C3aR信号调节抑郁症前额叶皮质的突触修剪。  |  Zhang, MM., et al. 2022. Cell Biosci. 12: 90. PMID: 35715851
  9. C3aR拮抗剂通过恢复肾素依赖性高血压中PPARα/CPT-1α介导的线粒体脂肪酸氧化，减轻C3a诱导的管状纤维化表型转变。  |  Wang, C., et al. 2023. Front Biosci (Landmark Ed). 28: 238. PMID: 37919077
  10. 在实验性蛛网膜下腔出血后，小胶质细胞通过C3/C3aR途径加重白质损伤。  |  Yang, L., et al. 2024. Exp Neurol. 379: 114853. PMID: 38866102
  11. 丝裂原活化蛋白激酶的激活需要来自人类无乳毒素 C5a 受体的两种信号输入。  |  Buhl, AM., et al. 1995. J Biol Chem. 270: 19828-32. PMID: 7649993
  12. 在实验性李斯特菌脑膜脑炎中，TNF-α介导的神经元上无形体毒素 C5a 受体的表达。  |  Stahel, PF., et al. 1997. J Immunol. 159: 861-9. PMID: 9218605

订购信息

产品名称产品编号规格价格数量收藏夹

C3aR 抗体 (D-12)

sc-133172
200 µg/ml
RMB2377.00

C3aR (D-12): m-IgG Fc BP-HRP 套装

sc-528759
200 µg Ab; 10 µg BP
RMB2662.00

C3aR (D-12): m-IgGκ BP-HRP 套装

sc-521261
200 µg Ab, 40 µg BP
RMB2662.00

C3aR (D-12): m-IgG2a BP-HRP 套装

sc-547158
200 µg Ab; 10 µg BP
RMB2662.00

C3aR 抗体 (D-12) AC

sc-133172 AC
500 µg/ml, 25% agarose
RMB3129.00

C3aR 抗体 (D-12) HRP

sc-133172 HRP
200 µg/ml
RMB2377.00

C3aR 抗体 (D-12) FITC

sc-133172 FITC
200 µg/ml
RMB2482.00

C3aR 抗体 (D-12) PE

sc-133172 PE
200 µg/ml
RMB2580.00

C3aR 抗体 (D-12) Alexa Fluor® 488

sc-133172 AF488
200 µg/ml
RMB2685.00

C3aR 抗体 (D-12) Alexa Fluor® 546

sc-133172 AF546
200 µg/ml
RMB2685.00

C3aR 抗体 (D-12) Alexa Fluor® 594

sc-133172 AF594
200 µg/ml
RMB2685.00

C3aR 抗体 (D-12) Alexa Fluor® 647

sc-133172 AF647
200 µg/ml
RMB2685.00

C3aR 抗体 (D-12) Alexa Fluor® 680

sc-133172 AF680
200 µg/ml
RMB2685.00

C3aR 抗体 (D-12) Alexa Fluor® 790

sc-133172 AF790
200 µg/ml
RMB2685.00

I have been using your Ab with variable results. I am worried that I am not using the correct reducing agent for my gel. In your blots, what reducing agent do you use?

Asked by: Minerva
Thank you for your question. We use our Electrophoresis Sample Buffer, 2X : sc-24945. Electrophoresis Sample Buffer contains DTT and TCEP-HCl, which disrupt disulfide bonds to ensure proteins are fully denatured before loading on the gel. Contains: glycerol (sc-29095), TCEP-HCl (sc-203290), DTT (sc-29089), SDS (sc-24950), MES (sc-255262), EDTA (sc-286969), Triton X-100 (sc-29112) and bromophenol blue (sc-24971). Please contact our Technical Service Department for assistance in troubleshooting this antibody.
Answered by: Technical Support
Date published: 2020-02-20

Can this antibody be used for cell immunofluorescence？

Asked by: apple20190529
This primary antibody is recommended and warrantied for IF (immunofluorescent staining of fresh or frozen tissues or cells fixed in methanol or acetone), however we are still gathering data to show on our website. Check back for updates!
Answered by: Technical Support
Date published: 2019-05-29

I need to use an antibody to detect the protein in samples of equine origin. Do you know if sc-133172: C3aR (D-12) mouse monoclonal antibody will work?

Asked by: AbPolly
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support
Date published: 2017-03-06
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Rated 5 out of 5 by from Remarkable effectthis is a very good antibody than I bought from other companies. thanks to santa, thanks for your good products. You should try your best to provide more antibodies of high quality like this to us researchers.
Date published: 2019-04-22
Rated 5 out of 5 by from Good choice for Western blotStrong specific single bands on Western blot, a reliable antibody for C3aR detection.
Date published: 2017-01-20
Rated 5 out of 5 by from Good performance in Western blotC3aR antibody was used to detect C3aR in rat tissue by WB, and a strong single band was detected.
Date published: 2017-01-09
Rated 5 out of 5 by from Single clean strong band in NIH/3T3 wholeSingle clean strong band in NIH/3T3 whole cell lysate. -SCBT QC
Date published: 2015-04-23
Rated 5 out of 5 by from Worked great for WesternWorked great for Western! Positive data in LPS treated RAW 264.7 and HeLa whole cell lysates. -SCBT QC
Date published: 2015-02-19
Rated 5 out of 5 by from WB: Clean band with M1 whole cell lysateWB: Clean band with M1 whole cell lysate. -SCBT QC
Date published: 2015-02-14
Rated 5 out of 5 by from Published WBPublished WB, IHC data with human placenta - SCBT Publication Review
Date published: 2014-09-16
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C3aR 抗体 (D-12) is rated 5.0 out of 5 by 7.
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