Date published: 2025-9-8
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c-Rel Antibody (B-6) - Western Blotting - Image 352427
c-Rel 抗体 (B-6): sc-6955. K-562 核提取物 (A), HeLa (B) 和 RAW 264. 7 (C) 全细胞裂解液中 c-Rel 表达的 Western 印迹分析.
c-Rel 抗体 (B-6): sc-6955. K-562 核提取物 (A), HeLa (B) 和 RAW 264. 7 (C) 全细胞裂解液中 c-Rel 表达的 Western 印迹分析.

c-Rel 抗体 (B-6): sc-6955

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寫評論提問

说明书
  • c-Rel抗体(B-6)是小鼠单克隆IgG1 λ c-Rel抗体, 在132篇文献中引用，规格为200 µg/ml
  • 免疫human来源c-Rel位于N-terminus 的1-300氨基酸
  • c-Rel 抗体 (B-6) 推荐用于 WB, IP, IF 和 IHC(P)，检测mouse, rat 和human 来源的 c-Rel p75
  • 抗c-Rel抗体（B-6）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-6955 X, 200 µg/0.1 ml)
  • 关于如何获取c-Rel (B-6): sc-6955的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgGλ BP-HRP (mouse IgGλ binding protein-HRP)是 c-Rel 抗体 (B-6) 在 WB and IHC(P) 应用中的首选二抗检测试剂。该试剂现与c-Rel 抗体 (B-6) 组合成套装提供。 (请参阅下面的订购信息)。其他m-IgGλ BP 偶联产品，请参阅我们的 小鼠 IgG 结合蛋白完整列表。

快捷链接

相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

c-Rel抗体（B-6）是一种小鼠单克隆IgG1 kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和免疫组织化学（IHC）检测小鼠、大鼠和人类来源的c-Rel。c-Rel抗体（B-6）有非结合型和多种结合型 ，包括琼脂糖、辣根过氧化物酶、藻红蛋白、异硫氰酸荧光素和多种Alexa Fluor®偶联物。c-Rel是核因子κ-轻链-活化B细胞（NFκB）信号传导通路的重要组成部分，该通路对于调节免疫反应、细胞增殖和存活至关重要。通过与目标基因启动子区域中的特定κB位点结合，c-Rel在免疫球蛋白κ轻链基因的转录激活中发挥着关键作用，从而影响B细胞的发展和功能。对c-Rel进行适当调控非常重要，因为失调会导致各种免疫失调并促进癌症发展。与v-Rel（缺乏功能性C端转激活结构域并破坏正常的NFκB信号传导）不同，c-Rel仍能发挥转录激活剂的作用，在维持细胞稳态和免疫功能方面意义重大。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

c-Rel 抗体 (B-6) 参考文献:

  1. IkappaBbeta 与 kappaB-Ras 共同抑制 NF-kappaB 的活性。  |  Chen, Y., et al. 2004. Mol Cell Biol. 24: 3048-56. PMID: 15024091
  2. c-rel 激活而 v-rel 抑制卡巴 B 位点的转录。  |  Inoue, J., et al. 1991. Proc Natl Acad Sci U S A. 88: 3715-9. PMID: 2023921
  3. 克隆 NF-kappa B 的 p50 DNA 结合亚基：与 rel 和 dorsal 同源。  |  Ghosh, S., et al. 1990. Cell. 62: 1019-29. PMID: 2203532
  4. 小鼠 c-rel 蛋白有一个 N 端调控结构域和一个 C 端转录激活结构域。  |  Bull, P., et al. 1990. Mol Cell Biol. 10: 5473-85. PMID: 2204816
  5. 细胞核和细胞质 rel 蛋白对基因表达的反式激活。  |  Hannink, M. and Temin, HM. 1989. Mol Cell Biol. 9: 4323-36. PMID: 2555689
  6. c-Rel及其在癌症中的多种作用：老故事新转折。  |  Hunter, JE., et al. 2016. Br J Cancer. 114: 1-6. PMID: 26757421
  7. 原癌基因 fos 的转录自动调节。  |  Sassone-Corsi, P., et al. 1988. Nature. 334: 314-9. PMID: 2839774
  8. v-rel癌基因产物在鸡成纤维细胞和脾脏细胞中的不同定位与REV-T的转化有关。  |  Gilmore, TD. and Temin, HM. 1986. Cell. 44: 791-800. PMID: 3004745
  9. c-Rel在纤维化中协调能量依赖性上皮和巨噬细胞的重编程。  |  Leslie, J., et al. 2020. Nat Metab. 2: 1350-1367. PMID: 33168981
  10. c-Rel依赖性Chk2信号传导可调节DNA损伤反应，从而限制肝癌的发生。  |  Leslie, J., et al. 2023. Hepatology. 78: 1050-1063. PMID: 36089330
  11. 网状内皮细胞增多症病毒转化骨髓细胞的分离和特征描述。  |  Franklin, RB., et al. 1974. Intervirology. 3: 342-52. PMID: 4443191

订购信息

产品名称产品编号规格价格数量收藏夹

c-Rel 抗体 (B-6)

sc-6955
200 µg/ml
RMB2377.00

c-Rel (B-6): m-IgGλ BP-HRP 套装

sc-520482
200 µg Ab, 40 µg BP
RMB2662.00

c-Rel 抗体 (B-6) X

sc-6955 X
200 µg/0.1 ml
RMB2377.00

c-Rel 抗体 (B-6) AC

sc-6955 AC
500 µg/ml, 25% agarose
RMB3129.00

c-Rel 抗体 (B-6) HRP

sc-6955 HRP
200 µg/ml
RMB2377.00

c-Rel 抗体 (B-6) FITC

sc-6955 FITC
200 µg/ml
RMB2482.00

c-Rel 抗体 (B-6) PE

sc-6955 PE
200 µg/ml
RMB2580.00

c-Rel 抗体 (B-6) Alexa Fluor® 488

sc-6955 AF488
200 µg/ml
RMB2685.00

c-Rel 抗体 (B-6) Alexa Fluor® 546

sc-6955 AF546
200 µg/ml
RMB2685.00

c-Rel 抗体 (B-6) Alexa Fluor® 594

sc-6955 AF594
200 µg/ml
RMB2685.00

c-Rel 抗体 (B-6) Alexa Fluor® 647

sc-6955 AF647
200 µg/ml
RMB2685.00

c-Rel 抗体 (B-6) Alexa Fluor® 680

sc-6955 AF680
200 µg/ml
RMB2685.00

c-Rel 抗体 (B-6) Alexa Fluor® 790

sc-6955 AF790
200 µg/ml
RMB2685.00

I have used this c-Rel antibody for WB and I've always gotten nonspecific two bands with 43 kDa and around 20 kDa. I followed the suggested protocol but cannot solve the problem. Any suggestions?

Asked by: mos6
Thank you for your question. It would be helpful if you could call 800-457-3801, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Service
Date published: 2022-09-22

When should the TransCruz reagent sc-6955 X be used instead of the standard form sc-6955?

Asked by: Trav11
Thank you for your question. We recommend using the TransCruz reagent form when performing ChIP or GelShift assays. This form can also be used in other applications such as Western Blot or IHC, but the dilution should be adjusted by a factor of 10 to account for the higher original concentration.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from 能做出条带我的实验中采用的是样本是RAW264.7细胞，从左到右依次为control（条带1和2）、knockout cell（条带3和4），可以看到都有条带的，大小和预期的接近。
Date published: 2018-03-08
Rated 1 out of 5 by from Not good for WBDidn't work in my hand, may be it work well under different stimulation conditions.
Date published: 2018-02-14
Rated 1 out of 5 by from doesn't work on WB, IHC, EMSAI normally used sc71 antibody for blots and everything which was working perfectly on mouse tissues and cell lines. To do EMSA I needed the concentrated antibody therefore ordered c-rel B6 antibody. Before we also received a sample antibody of B6 from SC. This antibody absolutely doesn't work neither on western blot, nor IHC and no shift in EMSA! I am not sure what to do, or if the problem is lot number. Sc71 antibody is amazing, unlike this one... I would really appreciate some feedback about it. Thank you
Date published: 2017-11-21
Rated 4 out of 5 by from c-Reli used this antibody for immunofluorescence staining and i got good result
Date published: 2017-09-22
Rated 5 out of 5 by from Positive Western Blot ResultsWe used this antibody for western blot staining and got a nice western blot at the correct molecular weight in HeLa cells
Date published: 2017-01-27
Rated 5 out of 5 by from Great quality as usual!Every member in our lab who has used this antibody says it is great as usual, we would like to use it in the future.
Date published: 2016-11-22
Rated 5 out of 5 by from WB positive with kyse150 cellsWB positive with kyse150 cells
Date published: 2016-09-12
Rated 4 out of 5 by from Published in WB with good data using humanPublished in WB with good data using human CD34+ PBPCs. -SCBT Publication Review
Date published: 2015-06-09
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c-Rel 抗体 (B-6) is rated 4.2 out of 5 by 17.
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