Date published: 2025-9-7

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c-Kit 抗体 (E-1): sc-17806

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说明书
  • c-Kit抗体(E-1)是小鼠单克隆IgG1 κ, 在19篇文献中引用,规格为200 µg/ml
  • 针对氨基酸23-322的抗原,靠近N-terminus
  • c-Kit 抗体 (E-1) 推荐用于WB, IP, IF 和 ELISA,检测human 来源的 c-Kit
  • 抗c-Kit抗体(E-1)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取c-Kit (E-1): sc-17806免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP1 BP-HRP">m-IgG1 BP-HRP是c-Kit Antibody (E-1) for WB applications. 的首选辅助检测试剂,这些试剂现与c-Kit Antibody (E-1) 打包提供(请参阅下面的订购信息)。

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関連項目

c-Kit抗体(E-1)是一种小鼠单克隆IgG1 kappa轻链抗体,用于通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)和酶联免疫吸附测定(ELISA)检测人类c-Kit。c-Kit单克隆抗体(E-1) 针对c-Kit N端附近的23-322个氨基酸,有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶(HRP)、藻红蛋白(PE)、异硫氰酸荧光素(FIT C)和多种Alexa Fluor®偶联物,确保在各种实验应用中的通用性。c-Kit,也称为CD117,是一种跨膜受体酪氨酸激酶,通过与干细胞因子(SCF)相互作用,在调节细胞趋化、增殖、凋亡和粘附等基本细胞过程中发挥着关键作用。c-Kit的翻译后修饰(包括糖基化和磷酸化)对于其正常功能和调节至关重要。c-Kit的糖基化对于稳定、正确折叠和细胞表面定位至关重要,这有助于有效的配体结合和受体二聚化。激酶结构域内特定酪氨酸残基的磷酸化对于激活信号转导通路至关重要,该通路可驱动造血、肥大细胞发育、黑色素生成和配子发生等细胞反应。这些修饰可确保c-Kit能够准确地将细胞外信号转化为适当的细胞反应,从而维持细胞内稳态和正常的生理功能。抗c-Kit抗体(E-1)能够有效结合并检测天然和修饰形式的c-Kit,因此成为研究人员研究信号传导机制、疾病病理学和靶向治疗干预的宝贵工具。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

c-Kit 抗体 (E-1) 参考文献:

  1. 干细胞因子受体/c-Kit的信号转导  |  Rönnstrand, L. 2004. Cell Mol Life Sci. 61: 2535-48. PMID: 15526160
  2. c-Kit 在人类正常干细胞和恶性干细胞中的表达 预后和治疗意义  |  Hassan, HT. 2009. Leuk Res. 33: 5-10. PMID: 18639336
  3. 造血生长因子受体 c-Kit 和 Flt3 发出的致癌信号  |  Masson, K. and Rönnstrand, L. 2009. Cell Signal. 21: 1717-26. PMID: 19540337
  4. 乳腺植物瘤中 c-Kit 的表达和突变  |  Bose, P., et al. 2010. Anticancer Res. 30: 4731-6. PMID: 21115932
  5. 在肺的维护和修复过程中,c-kit(+)细胞采用血管内皮细胞的命运,而不是上皮细胞的命运。  |  Liu, Q., et al. 2015. Nat Med. 21: 866-8. PMID: 26168292
  6. 猪生殖道中 C-kit 受体免疫阳性间质细胞(Cajal 型)。  |  Domino, M., et al. 2017. Acta Vet Scand. 59: 32. PMID: 28526042
  7. 在小鼠后肢缺血模型中,c-Kit功能丧失会损害动脉生成。  |  Hernandez, DR., et al. 2018. Surgery. 163: 877-882. PMID: 29287914
  8. 重新评估 c-Kit+ 细胞对成人心脏心肌细胞的贡献  |  He, L., et al. 2019. Circulation. 140: 164-166. PMID: 31283370
  9. RNA 聚合酶 I 抑制剂 BMH-21 和 BA-41 稳定 c-KIT G 型四联 DNA 结构  |  Mazzini, S., et al. 2019. Int J Mol Sci. 20: PMID: 31590335
  10. 坏死性小肠结肠炎与 C-KIT。  |  Yamataka, A., et al. 1998. J Pediatr Surg. 33: 1682-5. PMID: 9856895

订购信息

产品名称产品编号规格价格数量收藏夹

c-Kit 抗体 (E-1)

sc-17806
200 µg/ml
RMB2377.00

c-Kit (E-1): m-IgG Fc BP-HRP 套装

sc-526573
200 µg Ab; 10 µg BP
RMB2662.00

c-Kit (E-1): m-IgG1 BP-HRP 套装

sc-531946
200 µg Ab; 20 µg BP
RMB2662.00

c-Kit 抗体 (E-1) AC

sc-17806 AC
500 µg/ml, 25% agarose
RMB3129.00

c-Kit 抗体 (E-1) HRP

sc-17806 HRP
200 µg/ml
RMB2377.00

c-Kit 抗体 (E-1) FITC

sc-17806 FITC
200 µg/ml
RMB2482.00

c-Kit 抗体 (E-1) PE

sc-17806 PE
200 µg/ml
RMB2580.00

c-Kit 抗体 (E-1) Alexa Fluor® 488

sc-17806 AF488
200 µg/ml
RMB2685.00

c-Kit 抗体 (E-1) Alexa Fluor® 546

sc-17806 AF546
200 µg/ml
RMB2685.00

c-Kit 抗体 (E-1) Alexa Fluor® 594

sc-17806 AF594
200 µg/ml
RMB2685.00

c-Kit 抗体 (E-1) Alexa Fluor® 647

sc-17806 AF647
200 µg/ml
RMB2685.00

c-Kit 抗体 (E-1) Alexa Fluor® 680

sc-17806 AF680
200 µg/ml
RMB2685.00

c-Kit 抗体 (E-1) Alexa Fluor® 790

sc-17806 AF790
200 µg/ml
RMB2685.00

Does it cross react with rat?

Asked by: Ind13
Thank you for your question. c-Kit Antibody (E-1): sc-17806 is not recommended for use with rat cells or tissues, but cross-reactivity with such samples cannot be excluded. If you are looking for a rat-specific antibody please refer to c-Kit Antibody (E-3): sc-365504.
Answered by: Tech Support Europe
Date published: 2020-07-29

What is the recommended secondary reagent to use with c-Kit (E-1): sc-17806 for Western blot?

Asked by: jenni
We recommend using one our exclusive Mouse IgG Binding Proteins (m-IgGκ BP) conjugated to HRP as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Tech Service 3
Date published: 2017-01-27
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Rated 5 out of 5 by from Great results for WB and IHCGood strong bands for WB on and lots of publications available for WB and IHC.
Date published: 2016-12-22
Rated 5 out of 5 by from Detects cDetects c-Kit in WB. Strong band in CRISPR control and KO in sc-400106 transfected cells. -SCBT QC
Date published: 2015-05-04
Rated 5 out of 5 by from IP publication with mouse Ba/F3 transfected cellsIP publication with mouse Ba/F3 transfected cells. -SCBT Publication Review
Date published: 2015-04-29
Rated 5 out of 5 by from Worked for many applications: WB/IP/IF withWorked for many applications: WB/IP/IF with human HUVECs and HCMECs. -SCBT Publication Review
Date published: 2015-03-22
Rated 5 out of 5 by from Works well for Western in CCRFWorks well for Western in CCRF-HSB-2 and HEL 92.1.7 whole cell lysates. -SCBT QC
Date published: 2015-01-30
Rated 5 out of 5 by from Publication in IP and WB with HEK293 samplesPublication in IP and WB with HEK293 samples. -SCBT Publication Review
Date published: 2015-01-04
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c-Kit 抗体 (E-1) is rated 5.0 out of 5 by 6.
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