Date published: 2025-9-7

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c-Kit 抗体 (104D2): sc-19983

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说明书
  • c-Kit抗体(104D2)是小鼠单克隆IgG1 κ, 在8篇文献中引用,规格为200 µg/ml
  • 针对 human 巨核细胞系 MOLM-1 上表达的 CD117
  • 推荐用于 human 来源的c-Kit IF 和 FCM检测
  • 还可偶联藻红蛋白 以及 FITC 用于IF, IHC(P) 和 FCM
  • 看 c-Kit (E-3): sc-365504,可查看有标记的 c-Kit 抗体,包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • 目前,我们还没有完成c-Kit 抗体 (104D2)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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関連項目

c-Kit抗体(104D2)是一种小鼠单克隆IgG1 kappa轻链抗体,专门针对人类巨核细胞系MOLM-1上表达的CD117,在免疫荧光(IF)和流式细胞术(FCM)应用中,在人类样本中表现出强烈的反应性。c-Kit蛋白(也称为CD117)是一种跨膜受体酪氨酸激酶,在趋化性、增殖、凋亡和粘附等多种细胞过程中发挥着关键作用。在结构上,c-Kit由负责配体结合的细胞外结构域、单个跨膜螺旋和细胞内酪氨酸激酶结构域组成,当其配体干细胞因子(SCF)激活时,细胞内酪氨酸激酶结构域会启动下游信号通路。这种结构配置对于其功能至关重要,因为细胞外结构域可确保精确识别和结合配体,而细胞内激酶结构域则传递信号,调节造血、肥大细胞发育、黑色素生成和配子发生等关键生物活动。c-Kit 结构的完整性对于维持正常的信号传导至关重要。任何改变或突变,尤其是激酶结构域的改变或突变,都可能导致异常信号传导,从而促进各种癌症(如胃肠道间质瘤(GIST)和某些白血病)的发展和进展。c-Kit (104D2) 单克隆抗体推荐用于研究肿瘤学、血液学和细胞生物学的研究人员。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

c-Kit 抗体 (104D2) 参考文献:

  1. 干细胞因子及其受体 C-kit 的生物学特性  |  Ashman, LK. 1999. Int J Biochem Cell Biol. 31: 1037-51. PMID: 10582338
  2. c-kit基因替代产品在控制精原细胞增殖和精子介导的卵子活化中的分子机制。  |  Rossi, P., et al. 2003. Andrologia. 35: 71-8. PMID: 12558531
  3. 可溶性 c-kit(s-kit)作为一种新型肿瘤标记物和治疗中枢神经系统生殖细胞瘤的可能分子靶点的综述。  |  Takeshima, H. and Kuratsu, J. 2003. Surg Neurol. 60: 321-4; discussion 324-5. PMID: 14505850
  4. 颅内生殖细胞瘤中的 c-kit 基因突变。  |  Sakuma, Y., et al. 2004. Cancer Sci. 95: 716-20. PMID: 15471556
  5. c-Kit 对小鼠肺泡的维持和肺气肿样疾病的保护至关重要。  |  Lindsey, JY., et al. 2011. Am J Respir Crit Care Med. 183: 1644-52. PMID: 21471107
  6. C-KIT 信号传导依赖于小眼球相关转录因子对细胞增殖的影响。  |  Phung, B., et al. 2011. PLoS One. 6: e24064. PMID: 21887372
  7. 重新评估心脏细胞中的 c-Kit: 表达、命运和功能之间的复杂相互作用  |  Zhou, B. and Wu, SM. 2018. Circ Res. 123: 9-11. PMID: 29929968
  8. 原癌基因 c-Kit 作为依赖性受体抑制肿瘤生长  |  Wang, H., et al. 2018. Mol Cell. 72: 413-425.e5. PMID: 30293784
  9. 犬皮肤肥大细胞瘤中 KIT 蛋白表达和 c-kit 基因突变的预后和预测意义: 肿瘤学-病理学工作组的共识。  |  Thamm, DH., et al. 2019. Vet Comp Oncol. 17: 451-455. PMID: 31264352
  10. Wsh/Wsh c-kit 突变小鼠的 Cajal 间质细胞。  |  Iino, S., et al. 2020. J Smooth Muscle Res. 56: 58-68. PMID: 33132281

订购信息

产品名称产品编号规格价格数量收藏夹

c-Kit 抗体 (104D2)

sc-19983
200 µg/ml
RMB2377.00

c-Kit 抗体 (104D2) FITC

sc-19983 FITC
200 µg/ml
RMB2482.00

c-Kit 抗体 (104D2) PE

sc-19983 PE
200 µg/ml
RMB2580.00

How can c-Kit (104D2): sc-19983 mouse monoclonal antibody be used for double or triple staining, if the other primary antibodies are also raised in mouse?

Asked by: Trav11
In this case, we recommend using a directly conjugated mouse monoclonal primary antibody. This antibody is available conjugated to phycoerythrin (sc-19983 PE) or fluoroscein (sc-19983 FITC).
Answered by: Technical Support
Date published: 2017-02-27
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Rated 4 out of 5 by from Strong flow cytometry data in human HNSCC and USCStrong flow cytometry data in human HNSCC and USC-HN2 cell lines. -SCBT Publication Review
Date published: 2015-05-07
Rated 4 out of 5 by from Good publishable data in flow in rat PDP cellsGood publishable data in flow in rat PDP cells. -SCBT Publication Review
Date published: 2015-04-01
Rated 4 out of 5 by from Worked well in flow cytometry in human HeLaWorked well in flow cytometry in human HeLa, HUT102, Raji, FaDu, and SW579 cell lines. -SCBT Publication Review
Date published: 2015-02-09
Rated 4 out of 5 by from Wonderful FCM analysis of TFWonderful FCM analysis of TF-1 cells. -SCBT QC
Date published: 2013-07-16
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c-Kit 抗体 (104D2) is rated 4.0 out of 5 by 4.
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