Date published: 2025-9-7

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c-IAP1 抗体 (F-4): sc-271419

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说明书
  • c-IAP1 抗体 F-4 是小鼠单克隆 IgG1 κ,c-IAP1抗体, 在20篇文献中引用,规格为200 µg/ml
  • 针对human起源的凋亡蛋白1的c-IAP1 (inhibitor of apoptosis protein 1)内部区域内的氨基酸111-193进行
  • c-IAP1 抗体 (F-4) 推荐用于WB, IP, IF 和 ELISA,检测human 来源的 c-IAP1
  • 抗c-IAP1抗体(F-4)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取c-IAP1 (F-4): sc-271419免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP1 BP-HRP">m-IgG1 BP-HRPm-IgGκ BP-HRP是c-IAP1 Antibody (F-4) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与c-IAP1 Antibody (F-4) 打包提供(请参阅下面的订购信息)。

快捷链接

関連項目

c-IAP1抗体(F-4)是一种小鼠单克隆IgG1 kappa轻链抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)和酶联免疫吸附测定(ELISA)检测人源c-IAP1蛋白。c-IAP1抗体(F-4)有非结合型和多种 结合形式,包括琼脂糖、辣根过氧化物酶(HRP)、藻红蛋白(PE)、异硫氰酸荧光素(FITC)和多种Alexa Fluor®结合物。c-IAP1,也称为BIRC2,在调节细胞凋亡中起着至关重要的作用,细胞凋亡是维持细胞稳态和免疫反应所必需的程序性细胞死亡过程。通过抑制细胞凋亡,c-IAP1有助于防止细胞过早死亡,特别是在炎症和感染期间,使免疫细胞得以存活,从而有效发挥免疫反应。在癌症中,这种功能尤为重要,因为细胞凋亡的逃避会导致肿瘤生长和治疗抵抗。c-IAP1通过与关键信号分子(包括TRAF1和TRAF2)相互作用,发挥抗凋亡作用,这些分子参与肿瘤坏死因子(TNF)信号通路。c-IAP1调节这些相互作用的能力在正常生理过程和病理条件下都具有重要意义,使其成为治疗干预的重要靶点。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

c-IAP1 抗体 (F-4) 参考文献:

  1. 肿瘤坏死因子受体 2 信号诱导选择性 c-IAP1 依赖性 ASK1 泛素化,并终止丝裂原活化蛋白激酶信号传导。  |  Zhao, Y., et al. 2007. J Biol Chem. 282: 7777-82. PMID: 17220297
  2. c-IAP1 结合并处理 PCSK9 蛋白:将 TNF-α 途径中的 c-IAP1 与 PCSK9 介导的 LDLR 降解途径联系起来。  |  Xu, W., et al. 2012. Molecules. 17: 12086-101. PMID: 23085658
  3. 细胞凋亡抑制因子 BIRC2 核聚集和靶向的分子基础。  |  Tencer, AH., et al. 2023. Nat Struct Mol Biol. 30: 1265-1274. PMID: 37524969
  4. NAP1L1 通过 E3 泛素连接酶 UBR4 调节 BIRC2 泛素化修饰,从而决定肝细胞癌的进展。  |  Zhang, SL., et al. 2024. Cell Death Discov. 10: 154. PMID: 38538582
  5. 杆状病毒 p35 蛋白可抑制 Fas 和肿瘤坏死因子诱导的细胞凋亡。  |  Beidler, DR., et al. 1995. J Biol Chem. 270: 16526-8. PMID: 7542648
  6. 杆状病毒抗凋亡蛋白 p35 对 ICE 家族蛋白酶的抑制。  |  Bump, NJ., et al. 1995. Science. 269: 1885-8. PMID: 7569933
  7. 通过杆状病毒 p35 蛋白中的 CED-3 裂解位点抑制秀丽隐杆线虫细胞死亡蛋白酶 CED-3。  |  Xue, D. and Horvitz, HR. 1995. Nature. 377: 248-51. PMID: 7675111
  8. 表达杆状病毒 P35 可防止果蝇细胞死亡。  |  Hay, BA., et al. 1994. Development. 120: 2121-9. PMID: 7925015
  9. 杆状病毒基因 p35 和 iap 对程序性细胞死亡的控制。  |  Clem, RJ. and Miller, LK. 1994. Mol Cell Biol. 14: 5212-22. PMID: 8035800
  10. TNFR2-TRAF 信号复合体包含两个与杆状病毒凋亡抑制剂蛋白有关的新型蛋白。  |  Rothe, M., et al. 1995. Cell. 83: 1243-52. PMID: 8548810
  11. 克隆和表达具有抑制细胞凋亡和/或结合肿瘤坏死因子受体相关因子功能的细胞凋亡抑制蛋白同源物。  |  Uren, AG., et al. 1996. Proc Natl Acad Sci U S A. 93: 4974-8. PMID: 8643514
  12. 与杆状病毒 iap 基因有关并编码细胞凋亡抑制剂的细胞基因保守家族。  |  Duckett, CS., et al. 1996. EMBO J. 15: 2685-94. PMID: 8654366

订购信息

产品名称产品编号规格价格数量收藏夹

c-IAP1 抗体 (F-4)

sc-271419
200 µg/ml
RMB2377.00

c-IAP1 (F-4): m-IgG Fc BP-HRP 套装

sc-529182
200 µg Ab; 10 µg BP
RMB2662.00

c-IAP1 (F-4): m-IgGκ BP-HRP 套装

sc-521940
200 µg Ab, 40 µg BP
RMB2662.00

c-IAP1 (F-4): m-IgG1 BP-HRP 套装

sc-543375
200 µg Ab; 20 µg BP
RMB2662.00

c-IAP1 抗体 (F-4) AC

sc-271419 AC
500 µg/ml, 25% agarose
RMB3129.00

c-IAP1 抗体 (F-4) HRP

sc-271419 HRP
200 µg/ml
RMB2377.00

c-IAP1 抗体 (F-4) FITC

sc-271419 FITC
200 µg/ml
RMB2482.00

c-IAP1 抗体 (F-4) PE

sc-271419 PE
200 µg/ml
RMB2580.00

c-IAP1 抗体 (F-4) Alexa Fluor® 488

sc-271419 AF488
200 µg/ml
RMB2685.00

c-IAP1 抗体 (F-4) Alexa Fluor® 546

sc-271419 AF546
200 µg/ml
RMB2685.00

c-IAP1 抗体 (F-4) Alexa Fluor® 594

sc-271419 AF594
200 µg/ml
RMB2685.00

c-IAP1 抗体 (F-4) Alexa Fluor® 647

sc-271419 AF647
200 µg/ml
RMB2685.00

c-IAP1 抗体 (F-4) Alexa Fluor® 680

sc-271419 AF680
200 µg/ml
RMB2685.00

c-IAP1 抗体 (F-4) Alexa Fluor® 790

sc-271419 AF790
200 µg/ml
RMB2685.00

What is the recommended starting dilution for Western blot for c-IAP1 (F-4): sc-271419 monoclonal antibody?

Asked by: jenniferc
Thank you for your question. The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, can be found in the “datasheet” link at the top of the product page, for which I have provided the link: https://datasheets.scbt.com/sc-271419.pdf If you have any further questions or concerns, please contact our Technical Service department by calling 800-457-3801 option 2, emailing [email protected], or using the Live Chat function on our website.
Answered by: Technical Support 5
Date published: 2017-01-20
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Rated 4 out of 5 by from Good performance for WB!I tried 1:500 for cIAP1 overexpression in HEK293T cells and it works perfectly. The band is clean and sharp, without background.
Date published: 2017-09-28
Rated 5 out of 5 by from positive bandI got the target bands at 70kda by WB,diluted the antibody 1:1000,Hela cell used for positive control.We got positive band.
Date published: 2017-09-03
Rated 5 out of 5 by from good for WBTried 1:200 ,1:500, and 1:1000 dilution.I suggest use the dilution around 1:500,stong signal and clean and no background.
Date published: 2017-08-26
Rated 5 out of 5 by from Very clean resultc-IAP1 the antibody is very suitable for me, I can even see good bands at a low diluted condition (1:2000), Very clean and stable bands without any background.
Date published: 2017-05-28
Rated 5 out of 5 by from perfect antibodyWorks perfectly in HeLa whole cell lysates, high recommend 1:500 dilution
Date published: 2017-04-26
Rated 5 out of 5 by from Great Antibodyc-IAP1 antibody works perfectly with 293T cells for WB. The band is sharp and strong.
Date published: 2017-04-17
Rated 5 out of 5 by from good c-IAP1 antibodyVery good c-IAP1 antibody for WB I suggest dilution this antibody at 1:800.
Date published: 2017-04-08
Rated 5 out of 5 by from Good Antibodyc-IAP1 antibody worked well in the human samples. It had powerful band detected in a large number of cell lines, the result satisfied.
Date published: 2017-02-07
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c-IAP1 抗体 (F-4) is rated 4.4 out of 5 by 14.
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