Date published: 2025-9-11
访问圣克鲁斯动物健康

021-6093-6350

SCBT Portrait Logo
Seach Input
联系我们
登陆账户
快速订购
购物车 (0)
BSEP Antibody (F-6) - Western Blotting - Image 17037
BSEP 抗体 (F-6): sc-74500. C3H/10T1/2 (A), c4 (B) 和 KNRK (C) 全细胞裂解液中 BSEP 表达的 Western 印迹分析.
BSEP 抗体 (F-6): sc-74500. C3H/10T1/2 (A), c4 (B) 和 KNRK (C) 全细胞裂解液中 BSEP 表达的 Western 印迹分析.

BSEP 抗体 (F-6): sc-74500

4.2(19)
寫評論提問

说明书
  • BSEP 抗体 F-6 是小鼠单克隆 IgG2a κ，BSEP抗体, 在60篇文献中引用，规格为200 µg/ml
  • 免疫human物种的BSEP的氨基酸1-180
  • BSEP 抗体 (F-6) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 BSEP
  • 抗BSEP抗体（F-6）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取BSEP (F-6): sc-74500的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG2a BP-HRP 是 BSEP 抗体 (F-6) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与BSEP 抗体 (F-6) 组合成套装提供。 (请参阅下面的订购信息)。

快捷链接

相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

BSEP 抗体（F-6）是一种小鼠单克隆 IgG2a kappa 轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、免疫组织化学和酶联免疫吸附测定（ELISA）检测小鼠、大鼠和人类来源的 BSEP 蛋白。BSEP (F-6) 抗体有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 结合物。胆盐外排泵（BSEP）也称为ABCB11，在胆汁酸穿过肝细胞管状膜的过程中起着至关重要的作用，而胆汁酸的生成和稳态调节对胆汁的形成至关重要。BSEP位于肝脏，它促进胆汁盐从血液中分泌到胆汁中，从而防止胆汁酸在肝脏中积累。BSEP的正常运作对于消化和吸收膳食脂肪和脂溶性维生素至关重要，而BSEP功能障碍会导致胆汁淤积和肝脏疾病。此外，BSEP含有多种激酶的潜在磷酸化位点，表明BSEP活性可能通过翻译后修饰来调节，以响应胆盐浓度，这进一步强调了BSEP在维持肝脏健康和代谢平衡方面的重要性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

BSEP 抗体 (F-6) 参考文献:

  1. 胆盐的肝转运  |  Kullak-Ublick, GA., et al. 2000. Semin Liver Dis. 20: 273-92. PMID: 11076396
  2. 多药耐药性相关蛋白 2 和胆盐输出泵介导的胆汁酸转运的特征。  |  Akita, H., et al. 2001. Biochim Biophys Acta. 1511: 7-16. PMID: 11248200
  3. 在杆状病毒系统中过表达的小鼠胆盐输出泵的特征。  |  Noe, J., et al. 2001. Hepatology. 33: 1223-31. PMID: 11343252
  4. 人类胆盐输出泵启动子受法尼类固醇 X 受体/胆汁酸受体的反式激活。  |  Ananthanarayanan, M., et al. 2001. J Biol Chem. 276: 28857-65. PMID: 11387316
  5. 第一和第三孕期人类胎盘中 MDR3, FIC1, BSEP, OATP-A, OATP-C, OATP-D, OATP-E 和 NTCP 基因转录本的半定量表达分析。  |  Patel, P., et al. 2003. Placenta. 24: 39-44. PMID: 12495658
  6. 牛磺胆硷诱导的大鼠胆汁淤积症中管腔 Bsep 的定位和转运功能受损  |  Crocenzi, FA., et al. 2003. Gut. 52: 1170-7. PMID: 12865277
  7. 健康高加索人, 原发性胆汁性肝硬化和原发性硬化性胆管炎的 BSEP 和 MDR3 单倍型结构。  |  Pauli-Magnus, C., et al. 2004. Hepatology. 39: 779-91. PMID: 14999697
  8. LPS 诱导的人类肝脏中 MRP2 和 BSEP 的下调是由转录后过程引起的。  |  Elferink, MG., et al. 2004. Am J Physiol Gastrointest Liver Physiol. 287: G1008-16. PMID: 15205115
  9. 胆盐输出泵：分子特性、功能和调节。  |  Arrese, M. and Ananthanarayanan, M. 2004. Pflugers Arch. 449: 123-31. PMID: 15578267
  10. 重度胆盐输出泵缺乏症：109 个家庭中存在 82 种不同的 ABCB11 突变。  |  Strautnieks, SS., et al. 2008. Gastroenterology. 134: 1203-14. PMID: 18395098
  11. 健康和疾病中的胆盐输出泵（BSEP）。  |  Kubitz, R., et al. 2012. Clin Res Hepatol Gastroenterol. 36: 536-53. PMID: 22795478
  12. 基因型与严重胆盐输出泵缺乏症的自然病史有关。  |  van Wessel, DBE., et al. 2020. J Hepatol. 73: 84-93. PMID: 32087350

订购信息

产品名称产品编号规格价格数量收藏夹

BSEP 抗体 (F-6)

sc-74500
200 µg/ml
RMB2377.00

BSEP (F-6): m-IgG Fc BP-HRP 套装

sc-525724
200 µg Ab; 10 µg BP
RMB2662.00

BSEP (F-6): m-IgG2a BP-HRP 套装

sc-546381
200 µg Ab; 10 µg BP
RMB2662.00

BSEP 抗体 (F-6) AC

sc-74500 AC
500 µg/ml, 25% agarose
RMB3129.00

BSEP 抗体 (F-6) HRP

sc-74500 HRP
200 µg/ml
RMB2377.00

BSEP 抗体 (F-6) FITC

sc-74500 FITC
200 µg/ml
RMB2482.00

BSEP 抗体 (F-6) PE

sc-74500 PE
200 µg/ml
RMB2580.00

BSEP 抗体 (F-6) Alexa Fluor® 488

sc-74500 AF488
200 µg/ml
RMB2685.00

BSEP 抗体 (F-6) Alexa Fluor® 546

sc-74500 AF546
200 µg/ml
RMB2685.00

BSEP 抗体 (F-6) Alexa Fluor® 594

sc-74500 AF594
200 µg/ml
RMB2685.00

BSEP 抗体 (F-6) Alexa Fluor® 647

sc-74500 AF647
200 µg/ml
RMB2685.00

BSEP 抗体 (F-6) Alexa Fluor® 680

sc-74500 AF680
200 µg/ml
RMB2685.00

BSEP 抗体 (F-6) Alexa Fluor® 790

sc-74500 AF790
200 µg/ml
RMB2685.00

この抗体は，BSEPタンパクの細胞外，細胞内どちらの部位を認識しますか？

Asked by: naoki
Thank you for your question, raised against amino acids 1-180 of BSEP of human origin. Which site is recognised depends on the sample, so please consult the literature yourself. Thank you for your cooperation. ご質問ありがとうございます。human由来のBSEPのアミノ酸1-180に対応します。どちらの部位を認識するのはサンプルによって違いますので自分で文献をご参照ください。宜しくお願い致します。
Answered by: Jiajun Z
Date published: 2024-10-29

What was the immunogen used to generate this antibody? The website states aa1-180 of BSEP, but I assume the immunogen was a shorter peptide.

Asked by: Sven
Thank you for your question. No epitope mapping has been performed for this antibody as of yet, so the antibody's exact binding site is not known.
Answered by: Tech Support Europe
Date published: 2023-09-20

For western blots using Anti-BSEP Antibody (F-6): sc-74500, what kind of buffers do you recommend for blocking and probing (i.e. TBST 5% milk, or Licor reagents)?

Asked by: Murph Dog
Thank you for your question. It would be helpful if you could call 800-457-3801, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Service
Date published: 2022-08-30

Following my previous question, I would love to know if there is publication using F-6 antibody on ELISA

Asked by: yumin
Thank you for your question. BSEP Antibody (F-6): sc-74500 is recommended for detection of BSEP of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA. However, there are currently no publications using this antibody to detect BSEP in ELISA. Please contact our technical service for further information.
Answered by: SCBT Heidelberg Support
Date published: 2019-01-06

I was using BSEP Antibody (F-6: sc-74500) to detect BSEP in my cell lysate through ELISA. I have proven BSEP expression with the same sample and F-6 antibody on Western blot. But no signal in ELISA. How come? I have tried up to 200 fold dilution with F-6

Asked by: yumin
Thank you for your message. Please contact our Technical Service. We are happy to help you troubleshoot the antibody.
Answered by: SCBT Heidelberg Support
Date published: 2018-12-21

I need to detect BSEP in porcine samples. Can I use this monoclonal to do it?

Asked by: Cweed
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: TechService7
Date published: 2016-12-21

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: Cweed
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: TechService7
Date published: 2016-12-21
  • y_2025, m_9, d_11, h_4CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_7
  • loc_zh_CN, sid_74500, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 104ms
  • QUESTIONS, PRODUCT
Rated 1 out of 5 by from Sehr schlechte, z.T. gar keine Ergebnisse im WBIm Vergleich zu BSEP Antikörpern von anderen Firmen bin ich sehr enttäuscht über die Ergebnisse im WB. Wir hatten bei unseren WB sehr, sehr schwache Banden oder gar keine Banden. Haben den AK mit PVDF und auch Nitrocellulosemembran getetstet. Bei den Blockierungslösungen haben wir sowohl Milchpulver als auch BSA getestet. Um den Hauch einer Bande zu sehen mussten wir mit der Verdünnung des AK auf 1:100 runtergehen.
Date published: 2023-03-13
Rated 1 out of 5 by from Segnale debole in IF sulle HepG2anticorpo usato per IF nelle seguetni condizioni: Cellule: HepG2_20000 cell/well MW6 BLocking: BSA 5%_2h_RT Ab primario: BSEP (sc-74500)_1: 50 in BSA 1%_ ON+4 Ab secondario:Anti mouse_FITC 1:1000 Hoechst: 1: 1000 ( 2 ug/ml) per 10' Commento: DAPI OK, BSEP segnale diffuso e aspecifico
Date published: 2021-09-29
Rated 5 out of 5 by from The antibody works wellThe most amazing thing about BSEP antibody is western blot analyses demonstrated that high specificity, sensitivity and convenience.
Date published: 2017-10-18
Rated 5 out of 5 by from great resultGreat antibody for IF! We obtained really good stains on our samples.
Date published: 2017-05-27
Rated 5 out of 5 by from worked welliHC(P) was used on a sample of human, dilution 1:200 (1 ml of cell lysate)，worked well, I'm satisfied.
Date published: 2017-05-24
Rated 4 out of 5 by from fast, sensitivenessThis antibody has a rapid, sensitive and specific antibody detection, which is very good for us.
Date published: 2017-05-23
Rated 5 out of 5 by from strong signalIs a good replacement for BSEP antibody with better sensitivity in WB, got clear band
Date published: 2017-05-10
Rated 5 out of 5 by from BSEP antibodyI used this antibody for immunoblotting, It works well clear band 160kDa.
Date published: 2017-05-07
  • y_2025, m_9, d_11, h_4
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_19
  • loc_zh_CN, sid_74500, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 15ms
  • REVIEWS, PRODUCT
BSEP 抗体 (F-6) is rated 4.2 out of 5 by 19.
  • y_2025, m_9, d_11, h_4
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_19
  • loc_zh_CN, sid_74500, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 101ms
  • REVIEWS, PRODUCT
021-6093-6350
访问 Santa Cruz Animal Health|访问 San Juan Ranch
Santa Cruz Biotechnology, Inc. 是生物医学研究市场产品开发的世界领导者。请拨打0086-021-60936350联系我们。
版权所有© 2007-2025 Santa Cruz Biotechnology, Inc. 保留所有权利。”Santa Cruz Biotechnology” 和Santa Cruz Biotechnology, Inc.的商标，”Santa Cruz Animal Health”, “San Juan Ranch”, “Supplement of Champions”, San Juan Ranch的商标，”UltraCruz”, “ChemCruz”, “ImmunoCruz”, “ExactaCruz”, 和 “EZ Touch”是Santa Cruz Biotechnology, Inc.的注册商标。所有商标均为其各自所有者的财产。