Date published: 2025-9-7
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BSA Antibody (2A3E6) - Western Blotting - Image 393560
BSA 抗体 (2A3E6) HRP: sc-32816 HRP. 牛 PBL (A) 和 MDBK (B) 全细胞裂解液中 BSA 表达的直接 Western 印迹分析.
BSA 抗体 (2A3E6) HRP: sc-32816 HRP. 牛 PBL (A) 和 MDBK (B) 全细胞裂解液中 BSA 表达的直接 Western 印迹分析.

BSA 抗体 (2A3E6): sc-32816

4.5(4)
寫評論提問

说明书
  • BSA 抗体 2A3E6 是小鼠单克隆 IgG1 κ，BSA抗体, 在2篇文献中引用，规格为200 µg/ml
  • 针对牛血清白蛋白
  • BSA 抗体 (2A3E6) 推荐用于WB, IP 和 IF，检测bovine 来源的 BSA; 不会与 human, rabbit, canine or guinea pig albumins产生交联反应
  • 抗BSA抗体（2A3E6）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取BSA (2A3E6): sc-32816的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP、 1 BP-HRP">m-IgG1 BP-HRP和m-IgGκ BP-HRP是BSA Antibody (2A3E6) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与BSA Antibody (2A3E6) 打包提供（请参阅下面的订购信息）。

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研究信息

関連項目

额外Albumin抗体，包括BSA

BSA抗体（2A3E6）是一种小鼠单克隆IgG1 kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）和免疫荧光（IF）检测牛源性BSA蛋白。BSA (2A3E6) 抗体有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 结合物。牛血清白蛋白（BSA）是牛体内含量最丰富的血浆蛋白，在维持血浆渗透压方面起着至关重要的作用，而血浆渗透压则可确保体液在血管内腔和身体组织之间正常分布。BSA的结构由一条折叠成心形构象的多肽链组成，这使得BSA能够与各种配体结合，包括脂肪酸、激素和药物。这种独特的结构有助于提高BSA的溶解度和稳定性，同时促进其作为载体蛋白的功能，使BSA成为实验室开发免疫测定法的重要试剂。BSA的众多官能团，特别是赖氨酸残基，使其能够与其他分子有效结合，从而提高其在各种生物化学应用中的实用性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

BSA 抗体 (2A3E6) 参考文献:

  1. 牛血清白蛋白（BSA）对酶水解纤维素的影响。  |  Wang, H., et al. 2013. Appl Biochem Biotechnol. 170: 541-51. PMID: 23553108
  2. 利用自动毛细管 Western 技术定量检测疫苗中残留的牛血清白蛋白 (BSA)。  |  Loughney, JW., et al. 2014. Anal Biochem. 461: 49-56. PMID: 24841366
  3. 牛血清白蛋白（BSA）包裹的氧化铁磁性纳米粒子作为姜黄素抗癌药物的生物相容性载体。  |  Nosrati, H., et al. 2018. Bioorg Chem. 76: 501-509. PMID: 29310081
  4. 聚（烯丙胺盐酸盐）（PAH）和牛血清白蛋白（BSA）蛋白质纳米结构逐层薄膜。  |  Sa, AWL., et al. 2018. J Nanosci Nanotechnol. 18: 3908-3915. PMID: 29442726
  5. 用于结直肠肿瘤治疗的表皮生长因子受体靶向西妥昔单抗-缬氨酸-瓜氨酸（vc）-多柔比星免疫共轭物-负载牛血清白蛋白（BSA）纳米颗粒。  |  Ye, Z., et al. 2021. Int J Nanomedicine. 16: 2443-2459. PMID: 33814909
  6. 牛血清白蛋白（BSA）蛋白质与阴阳离子混合表面活性剂的相互作用及其结构。  |  Saha, D., et al. 2021. Soft Matter. 17: 6972-6984. PMID: 34236073
  7. 阴离子存在时牛血清白蛋白 (BSA) 分子间的相互作用：小角中子散射研究。  |  Pandit, S., et al. 2022. J Biol Phys. 48: 237-251. PMID: 35416637
  8. 通过结合计算机模拟的各种光谱技术，评估乙基樟脑与模型蛋白质牛血清白蛋白（BSA）的结合特性。  |  Jiang, SL., et al. 2023. J Biomol Struct Dyn. 41: 7862-7873. PMID: 36152999
  9. 由贻贝启发的多功能牛血清白蛋白（BSA）涂层负载黄芩素（BAI），可增强骨生成和抗氧化压力，有望应用于植入物。  |  Yang, S., et al. 2023. Int J Biol Macromol. 229: 752-765. PMID: 36586655
  10. 1 型糖尿病和其他自身免疫性疾病中的牛血清白蛋白 (BSA) 抗体。  |  Füchtenbusch, M., et al. 1997. Exp Clin Endocrinol Diabetes. 105: 86-91. PMID: 9137938

订购信息

产品名称产品编号规格价格数量收藏夹

BSA 抗体 (2A3E6)

sc-32816
200 µg/ml
RMB2377.00

BSA (2A3E6): m-IgG Fc BP-HRP 套装

sc-528476
200 µg Ab; 10 µg BP
RMB2662.00

BSA (2A3E6): m-IgGκ BP-HRP 套装

sc-520866
200 µg Ab, 40 µg BP
RMB2662.00

BSA (2A3E6): m-IgG1 BP-HRP 套装

sc-542954
200 µg Ab; 20 µg BP
RMB2662.00

BSA 抗体 (2A3E6) AC

sc-32816 AC
500 µg/ml, 25% agarose
RMB3129.00

BSA 抗体 (2A3E6) HRP

sc-32816 HRP
200 µg/ml
RMB2377.00

BSA 抗体 (2A3E6) FITC

sc-32816 FITC
200 µg/ml
RMB2482.00

BSA 抗体 (2A3E6) PE

sc-32816 PE
200 µg/ml
RMB2580.00

BSA 抗体 (2A3E6) Alexa Fluor® 488

sc-32816 AF488
200 µg/ml
RMB2685.00

BSA 抗体 (2A3E6) Alexa Fluor® 546

sc-32816 AF546
200 µg/ml
RMB2685.00

BSA 抗体 (2A3E6) Alexa Fluor® 594

sc-32816 AF594
200 µg/ml
RMB2685.00

BSA 抗体 (2A3E6) Alexa Fluor® 647

sc-32816 AF647
200 µg/ml
RMB2685.00

BSA 抗体 (2A3E6) Alexa Fluor® 680

sc-32816 AF680
200 µg/ml
RMB2685.00

BSA 抗体 (2A3E6) Alexa Fluor® 790

sc-32816 AF790
200 µg/ml
RMB2685.00

for sc-32816 one table shows epitope as FL (bovine) but I can not seem to find out what FL indicates

Asked by: Darrell
Thank you for your question. In the epitope description on the antibody table, FL is an abbreviation for "full-length", indicating the antibody was raised against an antigen sequence that is the full length protein, rather than a small peptide representing a specific region (e.g., N-terminus or C-terminus). In the case of this specific antibody, it was raised against bovine serum albumin, and not a recombinant or synthetic protein sequence. The exact epitope recognized by this monoclonal antibody has not yet been determined.
Answered by: Technical Support
Date published: 2019-08-01

Are there any references for using this antibody in IHC(P)?

Asked by: hpatel
BSA Antibody (2A3E6): sc-32816 is only recommended for detection of BSA of bovine origin by WB, IP and IF. We do not recommend this antibody for IHC(P) applications. There currently is no published data indicating that the antibody would also be suitable for IHC(P).
Answered by: Tech Support Europe
Date published: 2019-07-17

What is the recommended fixation method for immunofluorescence staining with BSA (2A3E6): sc-32816 monoclonal antibody?

Asked by: Dr Ninau Qelp
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from Positive results in Western Blot analysisWe have tested this antibody in Western Blot against pure BSA samples. It worked really well with strong and clear signals around 65 kDa (dilution 1:1000 in PBS 0.1% non-fat-dried milk and 0.05% Tween20; 2h incubation at room temperature).
Date published: 2022-04-12
Rated 5 out of 5 by from Good sensitivityThese antibodies are very sensitive with very little background. I am delighted with this product.
Date published: 2017-06-02
Rated 5 out of 5 by from Excellent SensitivityI have tried many other suppliers which resulted in very low intensity bands, the Santa Cruz antibodies result in bright intense bands.
Date published: 2017-05-02
Rated 4 out of 5 by from Positive result in WB using purified bovinePositive result in WB using purified bovine serum albumin. -SCBT QC
Date published: 2014-01-26
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BSA 抗体 (2A3E6) is rated 4.5 out of 5 by 4.
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