Date published: 2025-9-6
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Brn-3a/BRN3A/POU4F1 Antibody (14A6) - Western Blotting - Image 403332
Brn-3a/BRN3A/POU4F1 抗体 (14A6) Alexa Fluor® 488: sc-8429 AF488. 对 Ca Ski (A), HEK293T (B) 和 K-562 (C) 全细胞裂解液中 Brn-3a 表达的直接荧光西部印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断.
Brn-3a/BRN3A/POU4F1 抗体 (14A6) Alexa Fluor® 488: sc-8429 AF488. 对 Ca Ski (A), HEK293T (B) 和 K-562 (C) 全细胞裂解液中 Brn-3a 表达的直接荧光西部印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断.

Brn-3a/BRN3A/POU4F1 抗体 (14A6): sc-8429

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说明书
  • Brn-3a/BRN3A/POU4F1 抗体 14A6 是小鼠单克隆 IgG2b κ，Brn-3a/BRN3A/POU4F1抗体, 在157篇文献中引用，规格为200 µg/ml
  • 免疫mouse物种的Brn-3a的氨基酸1-109
  • Brn-3a/BRN3A/POU4F1 抗体 (14A6) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 Brn-3a
  • 抗Brn-3a/BRN3A/POU4F1抗体（14A6）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-8429 X, 200 µg/0.1 ml)
  • 关于如何获取Brn-3a/BRN3A/POU4F1 (14A6): sc-8429的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG2b BP-HRP 和 m-IgGκ BP-HRP 是 Brn-3a/BRN3A/POU4F1 抗体 (14A6) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Brn-3a/BRN3A/POU4F1 抗体 (14A6) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 Brn 抗体 包括 Brn-2, Brn-3, Brn-3a, Brn-3b, Brn-3c和Brn-5

Brn-3a抗体（14A6）是一种小鼠单克隆IgG2b kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、免疫组织化学和酶联免疫吸附测定（ELISA）检测小鼠、大鼠和人类来源的Brn-3a蛋白。抗Brn-3a抗体（14A6）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。Brn-3a是转录因子Brn家族的一员，通过调节神经元分化与存活所需的基因表达，在中枢神经系统发育中发挥着关键作用。Brn-3a (14A6) 单克隆抗体可识别高度受限的神经元亚群，其中Brn-3a对于感觉神经元的正常形成和视网膜发育至关重要。Brn-3a的独特结构包括两个功能性转激活结构域，可增强转录刺激，因此Brn-3a对于早期胚胎发育中的目标基因表达至关重要。了解Brn-3a的功能和定位有助于阐明神经发生的基本机制及其在神经退行性疾病中的潜在影响。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Brn-3a/BRN3A/POU4F1 抗体 (14A6) 参考文献:

  1. Brn-3a，POU基因家族的一种神经元转录因子：其在癌症和血管生成中的参与。  |  Chiarugi, V., et al. 2002. Mol Biotechnol. 22: 123-7. PMID: 12405260
  2. 哺乳动物神经系统发育期细胞中特异表达的新型 POU 同源基因  |  Collum, RG., et al. 1992. Nucleic Acids Res. 20: 4919-25. PMID: 1357630
  3. POU4F1/Brn-3a和POU4F2/Brn-3b转录因子在发育中的心脏中发挥必不可少但部分冗余的作用。  |  Maskell, LJ., et al. 2017. Cell Death Dis. 8: e2861. PMID: 28594399
  4. 内分泌性下丘脑和垂体后叶的发育和存活需要神经元 POU 结构域因子 Brn-2。  |  Schonemann, MD., et al. 1995. Genes Dev. 9: 3122-35. PMID: 8543156
  5. 将 P19 小鼠胚胎癌细胞优先分化为平滑肌细胞。使用维甲酸和反义中枢神经系统特异性 POU 转录因子 Brn-2。  |  Suzuki, T., et al. 1996. Circ Res. 78: 395-404. PMID: 8593698
  6. Brn-3a 转录因子的两个激活结构域的不同活性取决于结合位点的环境。  |  Budhram-Mahadeo, V., et al. 1996. J Biol Chem. 271: 9108-13. PMID: 8621561
  7. POU结构域因子Brn-3b是大量视网膜神经节细胞发育所必需的。  |  Gan, L., et al. 1996. Proc Natl Acad Sci U S A. 93: 3920-5. PMID: 8632990
  8. 转录因子 Brn-3.1 和 Brn-3.2 在听觉和视觉系统发育中的作用。  |  Erkman, L., et al. 1996. Nature. 381: 603-6. PMID: 8637595
  9. Brn-3.0的表达可识别早期有丝分裂后中枢神经系统神经元和感觉神经前体。  |  Fedtsova, NG. and Turner, EE. 1995. Mech Dev. 53: 291-304. PMID: 8645597
  10. 一个氨基酸的变化就能将抑制性转录因子转化为激活因子。  |  Dawson, SJ., et al. 1996. J Biol Chem. 271: 11631-3. PMID: 8662774
  11. 转录因子 POU4F3 突变与人类遗传性进行性听力损失有关。  |  Vahava, O., et al. 1998. Science. 279: 1950-4. PMID: 9506947

订购信息

产品名称产品编号规格价格数量收藏夹

Brn-3a/BRN3A/POU4F1 抗体 (14A6)

sc-8429
200 µg/ml
RMB2377.00

Brn-3a/BRN3A/POU4F1 (14A6): m-IgG Fc BP-HRP 套装

sc-528231
200 µg Ab; 10 µg BP
RMB2662.00

Brn-3a/BRN3A/POU4F1 (14A6): m-IgGκ BP-HRP 套装

sc-520557
200 µg Ab, 40 µg BP
RMB2662.00

Brn-3a/BRN3A/POU4F1 (14A6): m-IgG2b BP-HRP 套装

sc-550009
200 µg Ab; 10 µg BP
RMB2662.00

Brn-3a/BRN3A/POU4F1 抗体 (14A6) X

sc-8429 X
200 µg/0.1 ml
RMB2377.00

Brn-3a/BRN3A/POU4F1 抗体 (14A6) AC

sc-8429 AC
500 µg/ml, 25% agarose
RMB3129.00

Brn-3a/BRN3A/POU4F1 抗体 (14A6) HRP

sc-8429 HRP
200 µg/ml
RMB2377.00

Brn-3a/BRN3A/POU4F1 抗体 (14A6) FITC

sc-8429 FITC
200 µg/ml
RMB2482.00

Brn-3a/BRN3A/POU4F1 抗体 (14A6) PE

sc-8429 PE
200 µg/ml
RMB2580.00

Brn-3a/BRN3A/POU4F1 抗体 (14A6) Alexa Fluor® 488

sc-8429 AF488
200 µg/ml
RMB2685.00

Brn-3a/BRN3A/POU4F1 抗体 (14A6) Alexa Fluor® 546

sc-8429 AF546
200 µg/ml
RMB2685.00

Brn-3a/BRN3A/POU4F1 抗体 (14A6) Alexa Fluor® 594

sc-8429 AF594
200 µg/ml
RMB2685.00

Brn-3a/BRN3A/POU4F1 抗体 (14A6) Alexa Fluor® 647

sc-8429 AF647
200 µg/ml
RMB2685.00

Brn-3a/BRN3A/POU4F1 抗体 (14A6) Alexa Fluor® 680

sc-8429 AF680
200 µg/ml
RMB2685.00

Brn-3a/BRN3A/POU4F1 抗体 (14A6) Alexa Fluor® 790

sc-8429 AF790
200 µg/ml
RMB2685.00

Dear Santa Cruz, We would like to do Peptide Blocking with from this Brn3A 14a6 antibody, do you have the peptide for that. Thanks in advance

Asked by: Anonymous
Thank you for your question. We do not offer a blocking peptide for this particular antibody.
Answered by: Tech Support Europe
Date published: 2025-08-27

What is the volume of the following product? I don't see the volume on the web site. Brn-3a/BRN3A/POU4F1 Antibody (14A6) Alexa Fluor® 647 sc-8429 AF647 200 µg/ml $350.00

Asked by: Jeremy Nathans
Thank you for your question. The volume is 1ml.
Answered by: BlakeJ
Date published: 2024-04-24

Which dilution of the antibody did you use for the Western Blots that you show here?

Asked by: BarbaraH
Thank you for your question. The antibody was used at its recommended starting dilution of 1:200 in UltraCruz® Blocking Reagent: sc-516214.
Answered by: Tech Support Europe
Date published: 2023-08-31

Which dilution of the antibody was used for the Western Blot?

Asked by: BarbaraH
Thank you for your question. Brn-3a (14A6) is recommended for detection of Brn-3a of mouse, rat and human origin by Western Blotting with a starting dilution of 1:200, and a dilution range of 1:100-1:1000.
Answered by: Tech Support Europe
Date published: 2023-08-31

Does this perform well on mouse and rat retinal flat mounts?

Asked by: RebeccaR
Thank you for your question. It would be helpful if you could call 800-457-3801, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Service
Date published: 2022-09-22

Hi, can this antibody be use on rat's retina in Brn3A detection using IHC staning?

Asked by: Fatt
Thank you for your question. Yes, this antibody is reactive with rat and can be used for IHC staining in retina tissue. As a reference, please refer to the following product citations, PMID's: 15968082 and 23166779.
Answered by: Technical Support
Date published: 2018-11-30

Is this antibody reactive with Brn-3a of chicken origin?

Asked by: AbPolly
Thank you for your inquiry. Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Service
Date published: 2016-12-20

What secondary should be used with this antibody for Western Blot?

Asked by: AbPolly
Thank you for your inquiry. We recommend using our Mouse IgG kappa light chain binding protein, sc-516102, or our new NIR binding proteins conjugated to CFL 680 or CFL 790 for fluorescent imaging systems, sc-516180 and sc-516181. A full list of our available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Service
Date published: 2016-12-20
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Rated 1 out of 5 by from Non ha funzionato in ImmunofluorescenzaHo comprato questo free sample in data 24/11/20, provato su gangli dorsali di topo in immunofluorescenza da tessuto fresh frozen. Purtroppo il segnale dell'anticorpo è aspecifico e dà un forte background anche a diverse diluizioni anche cambiando il secondario.
Date published: 2020-12-21
Rated 3 out of 5 by from Antibody reviewThis antibody worked with westernblottimg not IF in eye samples.
Date published: 2018-06-14
Rated 5 out of 5 by from Excellent fluorescent IHCI got a very good fluorescent IHC on mouse retina frozen section using this antibody at 1:100. Should be able to use it at higher dilution.
Date published: 2018-05-25
Rated 4 out of 5 by from Good antibodyThe anibody worked very well with eye sections for immuno-fluorescence analysis!
Date published: 2018-04-30
Rated 5 out of 5 by from express high levelsThis antibody works well in immunohistochemistry (in 1:100), it showing a strong signal and it is the expected result.
Date published: 2017-10-26
Rated 5 out of 5 by from 效果不错之前申请了试用装，客服态度很好，十分热情。抗体效果也不错。抗体按1:50稀释，多聚甲醛固定，常规染色。
Date published: 2017-10-12
Rated 3 out of 5 by from 阴性申请了试用装，态度很好，速度很快，但是大鼠眼球切片节细胞染色为阴性，免疫荧光，1:200，不知为什么
Date published: 2017-10-09
Rated 4 out of 5 by from Good antibody for WBI often use this antibody, Western blot 1:200 dilution, it works well, perfect and different batches of antibodies, the results are good
Date published: 2017-06-01
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Brn-3a/BRN3A/POU4F1 抗体 (14A6) is rated 4.0 out of 5 by 23.
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