Date published: 2025-9-6

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Brn-3/BRN3/POU4F 抗体 (A-4): sc-390780

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说明书
  • Brn-3/BRN3/POU4F 抗体 A-4 是小鼠单克隆 IgG1 κ,Brn-3/BRN3/POU4F抗体, 在5篇文献中引用,规格为200 µg/ml
  • 免疫原氨基酸序列321-400位于human物种的Brn-3a的C-terminus附近
  • Brn-3/BRN3/POU4F 抗体 (A-4) 推荐用于WB, IP, IF 和 ELISA ,检测 mouse, rat, human 和 来源的Brn-3a, Brn-3b and Brn-3c; 也和以下物种反应,包括: 和 bovine, porcine and avian
  • 抗Brn-3/BRN3/POU4F抗体(A-4)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于ChIP的TransCruz试剂(sc-390780 X, 200 µg/0.1 ml)
  • 关于如何获取Brn-3/BRN3/POU4F (A-4): sc-390780免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP是Brn-3/BRN3/POU4F Antibody (A-4) 适用于 WB 应用。 的首选辅助检测试剂。该试剂现与Brn-3/BRN3/POU4F Antibody (A-4) 搭配使用(请参阅下面的订购信息)。

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関連項目

Brn-3抗体(A-4)是一种IgG1 κ小鼠单克隆Brn-3抗体(也称为BRN3A抗体、BRN3B抗体、BRN3C抗体、POU4F1抗体或POU类4同源盒抗体),它通过蛋白质印迹、免疫沉淀、免疫荧光和酶联免疫吸附试验检测小鼠、大鼠和人的Brn-3蛋白。Brn-3抗体(A-4)既可以作为非偶联的抗Brn-3抗体形式提供,也可以作为多种偶联形式的抗Brn-3抗体提供,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。Brn转录因子家族存在于高度限制的神经元子集中,对中枢神经系统的早期胚胎发育至关重要。Brn-1和Brn-2是III类POU(Pit-Oct-Unc)结构域蛋白质,而Brn-3是IV类POU结构域蛋白质。已经描述了三种Brn-3蛋白质,分别称为Brn-3a、Brn-3b和Brn-3c。虽然Brn-3a和Brn-3c刺激转录,但Brn-3b通常起转录阻遏物的作用。然而,已显示Brn-3b而非Brn-3a可调节乙酰胆碱受体的表达。有趣的是,Brn-3a具有两个功能性的反式激活结构域,一个位于氨基末端,另一个位于羧基末端。Brn-2被认为参与平滑肌细胞的发育和分化。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Brn-3/BRN3/POU4F 抗体 (A-4) 参考文献:

  1. Brn-3a转录因子在调节人乳头瘤病毒基因表达和确定宫颈癌细胞的生长特征方面起着关键作用。  |  Ndisang, D., et al. 1999. J Biol Chem. 274: 28521-7. PMID: 10497216
  2. Brn-3a转录因子在体内调节宫颈癌细胞的生长中起着关键作用。  |  Ndisang, D., et al. 2001. Oncogene. 20: 4899-903. PMID: 11521202
  3. 哺乳动物神经系统发育期细胞中特异表达的新型 POU 同源基因  |  Collum, RG., et al. 1992. Nucleic Acids Res. 20: 4919-25. PMID: 1357630
  4. 中国首次报告由同心镰刀菌引起的白柑橘采后果实腐烂病。  |  Xiao, L., et al. 2022. Plant Dis.. PMID: 35984392
  5. 内分泌性下丘脑和垂体后叶的发育和存活需要神经元 POU 结构域因子 Brn-2。  |  Schonemann, MD., et al. 1995. Genes Dev. 9: 3122-35. PMID: 8543156
  6. 将 P19 小鼠胚胎癌细胞优先分化为平滑肌细胞。使用维甲酸和反义中枢神经系统特异性 POU 转录因子 Brn-2。  |  Suzuki, T., et al. 1996. Circ Res. 78: 395-404. PMID: 8593698
  7. Brn-3a 转录因子的两个激活结构域的不同活性取决于结合位点的环境。  |  Budhram-Mahadeo, V., et al. 1996. J Biol Chem. 271: 9108-13. PMID: 8621561
  8. POU结构域因子Brn-3b是大量视网膜神经节细胞发育所必需的。  |  Gan, L., et al. 1996. Proc Natl Acad Sci U S A. 93: 3920-5. PMID: 8632990
  9. 转录因子 Brn-3.1 和 Brn-3.2 在听觉和视觉系统发育中的作用。  |  Erkman, L., et al. 1996. Nature. 381: 603-6. PMID: 8637595
  10. Brn-3.0的表达可识别早期有丝分裂后中枢神经系统神经元和感觉神经前体。  |  Fedtsova, NG. and Turner, EE. 1995. Mech Dev. 53: 291-304. PMID: 8645597
  11. 一个氨基酸的变化就能将抑制性转录因子转化为激活因子。  |  Dawson, SJ., et al. 1996. J Biol Chem. 271: 11631-3. PMID: 8662774
  12. 转录因子 POU4F3 突变与人类遗传性进行性听力损失有关。  |  Vahava, O., et al. 1998. Science. 279: 1950-4. PMID: 9506947

订购信息

产品名称产品编号规格价格数量收藏夹

Brn-3/BRN3/POU4F 抗体 (A-4)

sc-390780
200 µg/ml
RMB2377.00

Brn-3/BRN3/POU4F (A-4): m-IgG Fc BP-HRP 套装

sc-526192
200 µg Ab; 10 µg BP
RMB2662.00

Brn-3/BRN3/POU4F 抗体 (A-4) X

sc-390780 X
200 µg/0.1 ml
RMB2377.00

Brn-3/BRN3/POU4F 抗体 (A-4) AC

sc-390780 AC
500 µg/ml, 25% agarose
RMB3129.00

Brn-3/BRN3/POU4F 抗体 (A-4) HRP

sc-390780 HRP
200 µg/ml
RMB2377.00

Brn-3/BRN3/POU4F 抗体 (A-4) FITC

sc-390780 FITC
200 µg/ml
RMB2482.00

Brn-3/BRN3/POU4F 抗体 (A-4) PE

sc-390780 PE
200 µg/ml
RMB2580.00

Brn-3/BRN3/POU4F 抗体 (A-4) Alexa Fluor® 488

sc-390780 AF488
200 µg/ml
RMB2685.00

Brn-3/BRN3/POU4F 抗体 (A-4) Alexa Fluor® 546

sc-390780 AF546
200 µg/ml
RMB2685.00

Brn-3/BRN3/POU4F 抗体 (A-4) Alexa Fluor® 594

sc-390780 AF594
200 µg/ml
RMB2685.00

Brn-3/BRN3/POU4F 抗体 (A-4) Alexa Fluor® 647

sc-390780 AF647
200 µg/ml
RMB2685.00

Brn-3/BRN3/POU4F 抗体 (A-4) Alexa Fluor® 680

sc-390780 AF680
200 µg/ml
RMB2685.00

Brn-3/BRN3/POU4F 抗体 (A-4) Alexa Fluor® 790

sc-390780 AF790
200 µg/ml
RMB2685.00

I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using with Brn-3 (A-4): sc-390780?

Asked by: DefinitelyNotMatt
We recommend using one of our our exclusive Mouse IgG Binding Proteins conjugated to CFL 680 or CFL 790. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support
Date published: 2017-02-27
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Rated 1 out of 5 by from IF doesn't workI got this antibody and tested on the BRN3B-tdTo reporter cells and it does not work!
Date published: 2022-06-24
Rated 1 out of 5 by from It didn't labelNo signal in sections or purified cells for IF 1:250.
Date published: 2022-06-14
Rated 4 out of 5 by from Works for IF on crosectionsSample: D59 hESC-derived retinal organoid // Fix: 4% PFA 30mins on ice // Permeabilization: PBS +0.1% triton 10mins @RT // Block: 10% serum in PBS + 0.1% tween-20 for 1 hour @RT // Dilution: 1:100 in 10% serum in 1X PBS + 0.1% tween-20 // Primary Incubation: overnight @4C // Secondary: Mouse polyclonal, Alexa Fluor 555 (Invitrogen A-31570)
Date published: 2020-02-07
Rated 5 out of 5 by from Brn-3 works!!!This antibody works perfectly in mouse retina tissue
Date published: 2019-06-28
Rated 5 out of 5 by from GreatUsed to detect microglial cells in retinal tissue section, worked fine.
Date published: 2016-11-18
Rated 5 out of 5 by from Produced positive Western blot data of BrnProduced positive Western blot data of Brn-3 expression in K-562 whole cell lysate. -SCBT QC
Date published: 2013-02-04
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Brn-3/BRN3/POU4F 抗体 (A-4) is rated 3.5 out of 5 by 6.
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