Date published: 2025-9-6
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Brn-2/BRN2/POU3F2 Antibody (B-2) - Western Blotting - Image 361585
Brn-2 抗体 (B-2): sc-393324. A-375 (A), MDA-MB-435S (B) 和 C6 (C) 全细胞裂解液中 Brn-2 表达的 Western 印迹分析.
Brn-2 抗体 (B-2): sc-393324. A-375 (A), MDA-MB-435S (B) 和 C6 (C) 全细胞裂解液中 Brn-2 表达的 Western 印迹分析.

Brn-2/BRN2/POU3F2 抗体 (B-2): sc-393324

4.1(8)
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说明书
  • Brn-2/BRN2/POU3F2 抗体 B-2 是小鼠单克隆 IgG1 κ，Brn-2/BRN2/POU3F2抗体, 在22篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于human物种的Brn-2的C-terminus的氨基酸414-443之间
  • Brn-2/BRN2/POU3F2 抗体 (B-2) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 Brn-2
  • 抗Brn-2/BRN2/POU3F2抗体（B-2）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-393324 X, 200 µg/0.1 ml)
  • 关于如何获取Brn-2/BRN2/POU3F2 (B-2): sc-393324的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 1 BP-HRP">m-IgG1 BP-HRP和m-IgGκ BP-HRP是Brn-2/BRN2/POU3F2 Antibody (B-2) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与Brn-2/BRN2/POU3F2 Antibody (B-2) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

附加Brn抗体，包括Brn-2, Brn-3, Brn-3a, Brn-3b, Brn-3c和Brn-5

Brn-2抗体（B-2）是一种IgG1 κ小鼠单克隆Brn-2抗体（也被命名为POU类3同源盒2（POU3F2）抗体、Oct-7抗体、POU3类神经转录因子F2抗体或BRN2抗体），它通过WB、IP、IF和ELISA检测小鼠、大鼠和人类来源的Brn-2蛋白。Brn-2抗体（B-2）既可作为非共轭抗Brn-2抗体形式提供，也可作为多种共轭形式的抗Brn-2抗体提供，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®共轭物。转录因子Brn家族存在于高度特定的神经元亚群中，并对中枢神经系统的早期胚胎发育起到至关重要的作用。Brn-1和Brn-2是III类POU结构域蛋白。它们在前脑发育过程中表达，并在大多数II-V层皮质神经元中共同表达。Brn-1和Brn-2似乎对皮质神经元的径向迁移起始起着关键性的控制作用。据认为，Brn-2参与平滑肌细胞的发育和分化。Brn-3则是IV类POU结构域蛋白。目前已知有三种Brn-3蛋白，分别被命名为Brn-3a、Brn-3b和Brn-3c。Brn-3a具有两个功能性的反式激活结构域，分别位于氨基末端和羧基末端。尽管Brn-3a和Brn-3c刺激转录，但Brn-3b通常作为转录抑制因子发挥作用。然而，已有研究表明，Brn-3b（而非Brn-3a）能够调节乙酰胆碱受体的表达。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Brn-2/BRN2/POU3F2 抗体 (B-2) 参考文献:

  1. 半胱氨酸串联蛋白调控 G 蛋白对 N 型钙通道的调节。  |  Magga, JM., et al. 2000. Neuron. 28: 195-204. PMID: 11086994
  2. POU结构域蛋白Brn-2通过神经元芳香族L-氨基酸脱羧酶基因启动子的转激活需要NF-Y结合。  |  Dugast, C. and Weber, MJ. 2001. Brain Res Mol Brain Res. 89: 58-70. PMID: 11311976
  3. POU 结构域因子对大脑皮层神经元迁移的转录调控  |  McEvilly, RJ., et al. 2002. Science. 295: 1528-32. PMID: 11859196
  4. 分离人类基因组脑-2/N-Oct 3 基因（POUF3）并将其分配到染色体 6q16 上。  |  Atanasoski, S., et al. 1995. Genomics. 26: 272-80. PMID: 7601453
  5. 中枢神经系统特异性POU转录因子Brn-2是哺乳动物神经细胞谱系形成所必需的。  |  Fujii, H. and Hamada, H. 1993. Neuron. 11: 1197-206. PMID: 8274283
  6. 将 P19 小鼠胚胎癌细胞优先分化为平滑肌细胞。使用维甲酸和反义中枢神经系统特异性 POU 转录因子 Brn-2。  |  Suzuki, T., et al. 1996. Circ Res. 78: 395-404. PMID: 8593698
  7. Brn-3a 转录因子的两个激活结构域的不同活性取决于结合位点的环境。  |  Budhram-Mahadeo, V., et al. 1996. J Biol Chem. 271: 9108-13. PMID: 8621561
  8. POU结构域因子Brn-3b是大量视网膜神经节细胞发育所必需的。  |  Gan, L., et al. 1996. Proc Natl Acad Sci U S A. 93: 3920-5. PMID: 8632990
  9. 转录因子 Brn-3.1 和 Brn-3.2 在听觉和视觉系统发育中的作用。  |  Erkman, L., et al. 1996. Nature. 381: 603-6. PMID: 8637595
  10. Brn-3.0的表达可识别早期有丝分裂后中枢神经系统神经元和感觉神经前体。  |  Fedtsova, NG. and Turner, EE. 1995. Mech Dev. 53: 291-304. PMID: 8645597
  11. 一个氨基酸的变化就能将抑制性转录因子转化为激活因子。  |  Dawson, SJ., et al. 1996. J Biol Chem. 271: 11631-3. PMID: 8662774
  12. 人类 III 类 POU 基因 POU3F1 和 POU3F3 位于染色体 1p34.1 和 3p14.2。  |  Sumiyama, K., et al. 1998. Mamm Genome. 9: 180-1. PMID: 9457692

订购信息

产品名称产品编号规格价格数量收藏夹

Brn-2/BRN2/POU3F2 抗体 (B-2)

sc-393324
200 µg/ml
RMB2377.00

Brn-2/BRN2/POU3F2 (B-2): m-IgGκ BP-HRP 套装

sc-523904
200 µg Ab, 40 µg BP
RMB2662.00

Brn-2/BRN2/POU3F2 (B-2): m-IgG1 BP-HRP 套装

sc-533621
200 µg Ab; 20 µg BP
RMB2662.00

Brn-2/BRN2/POU3F2 抗体 (B-2) X

sc-393324 X
200 µg/0.1 ml
RMB2377.00

Brn-2/BRN2/POU3F2 抗体 (B-2) AC

sc-393324 AC
500 µg/ml, 25% agarose
RMB3129.00

Brn-2/BRN2/POU3F2 抗体 (B-2) HRP

sc-393324 HRP
200 µg/ml
RMB2377.00

Brn-2/BRN2/POU3F2 抗体 (B-2) FITC

sc-393324 FITC
200 µg/ml
RMB2482.00

Brn-2/BRN2/POU3F2 抗体 (B-2) PE

sc-393324 PE
200 µg/ml
RMB2580.00

Brn-2/BRN2/POU3F2 抗体 (B-2) Alexa Fluor® 488

sc-393324 AF488
200 µg/ml
RMB2685.00

Brn-2/BRN2/POU3F2 抗体 (B-2) Alexa Fluor® 546

sc-393324 AF546
200 µg/ml
RMB2685.00

Brn-2/BRN2/POU3F2 抗体 (B-2) Alexa Fluor® 594

sc-393324 AF594
200 µg/ml
RMB2685.00

Brn-2/BRN2/POU3F2 抗体 (B-2) Alexa Fluor® 647

sc-393324 AF647
200 µg/ml
RMB2685.00

Brn-2/BRN2/POU3F2 抗体 (B-2) Alexa Fluor® 680

sc-393324 AF680
200 µg/ml
RMB2685.00

Brn-2/BRN2/POU3F2 抗体 (B-2) Alexa Fluor® 790

sc-393324 AF790
200 µg/ml
RMB2685.00

Brn-2/BRN2/POU3F2 (B-2) 中和勝肽

sc-393324 P
100 µg/0.5 ml
RMB511.00

What application is the blocking peptide sc-393324 P appropriate for?

Asked by: Professor Griffin
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from Works for IFI got a free sample to test this antibody for IF using free-floating brain sections from postnatal day 1 mice. It did not worked with regular immunostaining protocol, probably due to poor penetration of this antibody to nuclei. I finally got it to work by modifying the protocol. Here is a brief protocol I used: 1) wash sections 3 times in PBS. 2) pre-treat sections in 0.8% triton in PBS for 40 min. 3) Perform antigen retrieval (citrate buffer pH 9.0) 85 degree for 30 min, cool to RT. 4) block with 10% serum. 5) Incubate with primary antibody 1:400, overnight at 4 degree (with 0.3% triton). 6) secondary antibody biotin-anti-mouse IgG. 7) Avidin-alex488.
Date published: 2019-03-25
Rated 1 out of 5 by from Brn2 antibody does not work for IFClaims to work for IF but after following their protocols precisely and testing on multiple timepoints and samples (cortex) I was not able to get satisfactory signal. I would strongly NOT recommend. Furthermore I tried to troubleshoot with SCBT technical support and they were not helpful at all. Did not answer my questions and did not seem to even understand the problem. Complete waste of time.
Date published: 2018-02-21
Rated 5 out of 5 by from Works in Embryonic Neural Tissue for IHCWas able to get working well in embryonic PFA fixed neural tissue 1:200 O/N with Antigen Retrieval
Date published: 2017-08-17
Rated 5 out of 5 by from Great antibody!Works great for IF on brain tissue! Dilution is 1:200.
Date published: 2017-02-22
Rated 4 out of 5 by from StrongStrong, solid band observed by WB using SK-N-MC whole cell lysate. -SCBT QC
Date published: 2015-04-13
Rated 5 out of 5 by from Works well for ChIPWorks well for ChIP.
Date published: 2015-03-26
Rated 4 out of 5 by from Good band observed at expected molecularGood band observed at expected molecular weight for the Brn-2 protein by WB using HEK293 whole cell lysate. A couple of light non-specific bands observed; light background. -SCBT QC
Date published: 2015-03-25
Rated 5 out of 5 by from Clean backgroundClean background, crisp band observed by WB using Jurkat nuclear extract. -SCBT QC
Date published: 2015-03-16
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Brn-2/BRN2/POU3F2 抗体 (B-2) is rated 4.1 out of 5 by 8.
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