Date published: 2026-10-24

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SMARCA4/Brg1 Antibody (H-10) - Western Blotting - Image 353975
Brg-1 抗体 (H-10): sc-374197. MCF7 (A), Ramos (B) 和 Jurkat (C) 核提取物以及小鼠胸腺组织提取物 (D) 中 Brg-1 表达的 Western 印迹分析.
Brg-1 抗体 (H-10): sc-374197. MCF7 (A), Ramos (B) 和 Jurkat (C) 核提取物以及小鼠胸腺组织提取物 (D) 中 Brg-1 表达的 Western 印迹分析.

SMARCA4/Brg1 抗体 (H-10): sc-374197

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寫評論提問
说明书
  • SMARCA4/Brg1抗体(H-10)是小鼠单克隆IgG1 κ, 在17篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于human物种的Brg-1的N-terminus附近的氨基酸115-149之间
  • 抗-SMARCA4/Brg1 抗体 (H-10) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 Brg-1; 也和以下物种反应，包括： 和 canine, bovine and porcine
  • 可用于ChIP的TransCruz试剂(sc-374197 X, 200 µg/0.1 ml)
  • 抗SMARCA4/Brg1抗体（H-10）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • m-IgG Fc BP-HRP和 m-IgG1 BP-HRP是SMARCA4/Brg1 Antibody (H-10) for WB applications. 的首选辅助检测试剂，这些试剂现与SMARCA4/Brg1 Antibody (H-10) 打包提供（请参阅下面的订购信息）。
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研究信息

関連項目

Brg-1抗体（H-10）是一种IgG1 κ小鼠单克隆Brg-1抗体（也称为Brg1抗体），可通过WB、IP、IF和ELISA检测小鼠、大鼠和人源的Brg-1。Brg-1抗体（H-10）既可作为非偶联抗Brg-1抗体形式提供，也可作为多种偶联形式的抗Brg-1抗体提供，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。SWI-SNF复合物通过以ATP依赖的方式重塑核小体结构参与转录的激活。Brm（也称为SNF2α）和Brg-1（也称为SNF2β）是哺乳动物SWI-SNF复合物的ATP酶亚基。Brm、Brg-1、Ini1（整合酶相互作用蛋白1，也称为SNF5）、BAF155（也称为SRG3）和BAF170被认为构成了SWI-SNF复合物的功能核心。向Brg-1中添加Ini1、BAF155和BAF170似乎可以增加重塑活性。其他复合物亚基被认为起调节作用。hSNF2L和hSNF2H都似乎是果蝇ISWI的同源物，ISWI是一种与Brm相关的ATP酶，存在于除SWI/SNF以外的染色质重塑复合物中，包括NURF（核小体重塑因子）。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

SMARCA4/Brg1 抗体 (H-10) 参考文献:

  1. SWI/SNF复合物的BRG-1亚基调节CD44的表达。  |  Strobeck, MW., et al. 2001. J Biol Chem. 276: 9273-8. PMID: 11108719
  2. BRm对BRG-1功能的补偿：洞察核心SWI-SNF亚基在视网膜母细胞瘤肿瘤抑制信号传导中的作用。  |  Strobeck, MW., et al. 2002. J Biol Chem. 277: 4782-9. PMID: 11719516
  3. 一种推定的核受体辅助激活因子（TMF/ARA160）与 hbrm/hSNF2 alpha 和 BRG-1/hSNF2 beta 结合，并定位在高尔基体中。  |  Mori, K. and Kato, H. 2002. FEBS Lett. 520: 127-32. PMID: 12044884
  4. Prohibitin需要Brg-1和Brm来抑制E2F和细胞生长。  |  Wang, S., et al. 2002. EMBO J. 21: 3019-28. PMID: 12065415
  5. BRG-1的下调抑制了宫颈神经内分泌癌和腺癌中CD44s的表达。  |  Kuo, KT., et al. 2006. Mod Pathol. 19: 1570-7. PMID: 16998464
  6. Axl通过激活NF-κB和Brg-1诱导MMP-9活性，从而促进细胞侵袭。  |  Tai, KY., et al. 2008. Oncogene. 27: 4044-55. PMID: 18345028
  7. Brm和Brg-1 Swi/Snf ATP酶在肝胆酸生物合成反馈调节中的功能特异性。  |  Miao, J., et al. 2009. Mol Cell Biol. 29: 6170-81. PMID: 19805516
  8. Brg-1通过与转录因子Sp1相互作用，介导乳腺癌细胞中SPARC的组成型表达和芬瑞丁诱导型表达。  |  Xu, YZ., et al. 2010. Mol Cancer. 9: 210. PMID: 20687958
  9. 靶向SWI/SNF复合物的BRG-1/BRM亚基的溴odomain可增强替莫唑胺在胶质母细胞瘤中的抗癌活性  |  Yang, C., et al. 2021. Pharmaceuticals (Basel). 14: PMID: 34577604
  10. 作为人类SNF/SWI复合物组成部分的hbrm和BRG-1蛋白在细胞有丝分裂过程中被磷酸化并排除在浓缩的染色体之外。  |  Muchardt, C., et al. 1996. EMBO J. 15: 3394-402. PMID: 8670841

订购信息

产品名称产品编号规格价格数量收藏夹

SMARCA4/Brg1 抗体 (H-10)

sc-374197
200 µg/ml
CNY2422.00

SMARCA4/Brg1 (H-10): m-IgG Fc BP-HRP 套装

sc-527442
200 µg Ab; 10 µg BP
CNY2715.00

SMARCA4/Brg1 (H-10): m-IgG1 BP-HRP 套装

sc-532815
200 µg Ab; 20 µg BP
CNY2715.00

SMARCA4/Brg1 抗体 (H-10) X

sc-374197 X
200 µg/0.1 ml
CNY2422.00

SMARCA4/Brg1 抗体 (H-10) AC

sc-374197 AC
500 µg/ml, 25% agarose
CNY3189.00

SMARCA4/Brg1 抗体 (H-10) HRP

sc-374197 HRP
200 µg/ml
CNY2422.00

SMARCA4/Brg1 抗体 (H-10) FITC

sc-374197 FITC
200 µg/ml
CNY2527.00

SMARCA4/Brg1 抗体 (H-10) PE

sc-374197 PE
200 µg/ml
CNY2625.00

SMARCA4/Brg1 抗体 (H-10) Alexa Fluor® 488

sc-374197 AF488
200 µg/ml
CNY2738.00

SMARCA4/Brg1 抗体 (H-10) Alexa Fluor® 546

sc-374197 AF546
200 µg/ml
CNY2738.00

SMARCA4/Brg1 抗体 (H-10) Alexa Fluor® 594

sc-374197 AF594
200 µg/ml
CNY2738.00

SMARCA4/Brg1 抗体 (H-10) Alexa Fluor® 647

sc-374197 AF647
200 µg/ml
CNY2738.00

SMARCA4/Brg1 抗体 (H-10) Alexa Fluor® 680

sc-374197 AF680
200 µg/ml
CNY2738.00

SMARCA4/Brg1 抗体 (H-10) Alexa Fluor® 790

sc-374197 AF790
200 µg/ml
CNY2738.00

SMARCA4/Brg1 (H-10) 中和勝肽

sc-374197 P
100 µg/0.5 ml
CNY519.00

I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using with Brg-1 Antibody (H-10): sc-374197?

Asked by: Trav11
Thank you for your question. We recommend using one of our our exclusive Mouse IgG Binding Proteins (m-IgGκ) conjugated to CFL 680 or CFL 790. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety If you have any further questions or concerns, please feel free to contact our Technical Service department by calling 800-457-3801 option 2, emailing [email protected], or using the Live Chat function on our website.
Answered by: Technical Support 5
Date published: 2017-02-04
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Rated 4 out of 5 by Elhamdianati from Brg-1 (H-10) used for western blot applicationWorks! 1/500 in 3%BSA. Fainter bands than Brg1-G7.
Date published: 2018-03-07
Rated 4 out of 5 by aurelia from ParfaitTesté en WB sur fibroblastes embryonnaires murin, il est très spécifique!
Date published: 2018-01-03
Rated 5 out of 5 by cjMara from New choice for WB of Brg-1The H-10 is a good new choice for detection of Brg-1 in Western blot. However, the G-7 clone is much more popular having been featured in over 50 publications!
Date published: 2017-01-13
Rated 5 out of 5 by Master Wendu from Currently the best of the Brg1 antibodies we haveBrg1 (H10) is an excellent antibody (similar to G7). We have great success using it for IPs, even with BS3-crosslinking. It is also very nice for Western Blots.
Date published: 2016-12-16
Rated 5 out of 5 by Xinch from Good WB antibodyFor 293T cell lysate, very strong signal (1:100 dilution)
Date published: 2016-12-05
Rated 5 out of 5 by JT from Nice WB signal achieved in KNice WB signal achieved in K-562 nuclear extract. -SCBT QC
Date published: 2015-03-30
Rated 5 out of 5 by Xiaomin from Very clean and specificVery clean and specific, gave beautiful bands in Western Blot.
Date published: 2015-03-25
Rated 4 out of 5 by JM from Beautiful band acquired in HeLa whole cell lysateBeautiful band acquired in HeLa whole cell lysate. -SCBT QC
Date published: 2015-03-22
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SMARCA4/Brg1 抗体 (H-10) is rated 4.7 out of 5 by 9.
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