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BrdU Antibody (Bu20A) - Flow Cytometry - Image 9912
BrdU 抗体 (Bu20A) PE: sc-20045 PE. 对固定和通透的未处理 (蓝色虚线直方图) 和经 BrdU 处理 (橙色实线直方图) 的 K-562 细胞进行胞内 FCM 分析. 绿线直方图代表同型对照, 正常小鼠 IgG1: sc-2866.
BrdU Antibody (Bu20A) - Flow Cytometry - Image 9912
BrdU 抗体 (Bu20A) PE: sc-20045 PE. 对固定和通透的未处理 (蓝色虚线直方图) 和经 BrdU 处理 (橙色实线直方图) 的 K-562 细胞进行胞内 FCM 分析. 绿线直方图代表同型对照, 正常小鼠 IgG1: sc-2866.
BrdU 抗体 (Bu20A) PE: sc-20045 PE. 对固定和通透的未处理 (蓝色虚线直方图) 和经 BrdU 处理 (橙色实线直方图) 的 K-562 细胞进行胞内 FCM 分析. 绿线直方图代表同型对照, 正常小鼠 IgG1: sc-2866.

BrdU 抗体 (Bu20A): sc-20045

4.5(4)
寫評論提問

说明书
  • BrdU抗体(Bu20A)是小鼠单克隆IgG1 κ, 在12篇文献中引用，规格为200 µg/ml
  • 培养自结合载体蛋白的bromodeoxyuridine (BrdU)
  • 推荐用于检测BrdU, a proliferation marker incorporated into newly synthesized DNA during S-phase of a cell cycle,（由FCM提供
  • 识别标记了BrdU的细胞的变性DNA中的BrdU
  • 推荐配套产品：5-Bromo-2′ deoxyuridine: sc-290815
  • 还可偶联藻红蛋白 以及 FITC 用于IF, IHC(P) 和 FCM
  • 看 BrdU (IIB5): sc-32323，可查看有标记的 BrdU 抗体，包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • 目前，我们还没有完成BrdU 抗体 (Bu20A)的首选二抗检测试剂的鉴定。这项工作正在进行中。

快捷链接

描述
说明书与实验方案
研究信息

関連項目

卤化嘧啶胸苷类似物溴脱氧尿嘧啶(BrdU)被掺入S期细胞新合成的DNA链中，可用于估算S期细胞的比例。此外，分析BrdU的摄入量是定量DNA合成程度的可靠方法。BrdU也可用于研究姐妹染色单体交换和分离新生DNA。紫外线诱导的切除修复合成是将BrdU掺入细胞DNA的一种方法。抗BrdU抗体在盐酸变性步骤或核酸酶消化后，与单链DNA中暴露的BrdU结合。对于大多数用交联剂如福尔马林固定的组织或细胞，蛋白酶修复是必要的，但可能会降低BrdU免疫检测的特异性。

仅限研究使用。不适用于诊断和治疗用途。

订购信息

产品名称产品编号规格价格数量收藏夹

BrdU 抗体 (Bu20A)

sc-20045
200 µg/ml
RMB2377.00

BrdU 抗体 (Bu20A) FITC

sc-20045 FITC
200 µg/ml
RMB2482.00

BrdU 抗体 (Bu20A) PE

sc-20045 PE
200 µg/ml
RMB2580.00

Can BrdU (Bu20A): sc-20045 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: jenniferc
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, BrdU (Bu20A): sc-20045 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2019-07-02
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Rated 5 out of 5 by from Conjugate has Good SignalGood detection of BrdU positive cells seen when using the PE conjugated version in Flow Cytometry.
Date published: 2017-01-27
Rated 4 out of 5 by from IHCP published data with mouse cerebellumIHCP published data with mouse cerebellum -SCBT Publication Review
Date published: 2015-06-02
Rated 5 out of 5 by from Antibody was published for use in IHC onAntibody was published for use in IHC on mice stem cell samples. Worked well! -SCBT Publication Review
Date published: 2015-02-21
Rated 4 out of 5 by from Produced great intracellular FCM data forProduced great intracellular FCM data for fixed and permeabilized untreated and BrdU-treated K-562 cells. -SCBT QC
Date published: 2014-12-10
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BrdU 抗体 (Bu20A) is rated 4.5 out of 5 by 4.
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