Date published: 2025-9-8

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BRD2 抗体 (G-4): sc-393720

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说明书
  • BRD2 抗体 G-4 是小鼠单克隆 IgG1 κ,BRD2抗体, 在6篇文献中引用,规格为200 µg/ml
  • 特异性抗原位于human物种的BRD2的N-terminus的氨基酸1-22之间
  • BRD2 抗体 (G-4) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 BRD2; 也和以下物种反应,包括: 和 equine and bovine
  • 抗BRD2抗体(G-4)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取BRD2 (G-4): sc-393720免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRPm-IgGκ BP-HRP 是 BRD2 抗体 (G-4) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与BRD2 抗体 (G-4) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

BRD2抗体(G-4)是一种IgG1 κ小鼠单克隆BRD2抗体,可通过WB、IP、IF、IHC(P)和ELISA方法检测小鼠、大鼠和人类来源的BRD2蛋白。BRD2抗体(G-4)既可作为非偶联抗BRD2抗体形式提供,也可作为多种偶联形式的抗BRD2抗体提供,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。含溴结构域的蛋白质包括BRD2、BRD3、BRD4和BRDT。BRD2(RING3蛋白)是一种丝裂原激活的核蛋白,其基因位于人类MHC II区域,提示其与HLA相关疾病的关系。编码BRD3(RING3样蛋白)的基因含有两个溴结构域,并定位于9号染色体q34。BRD4(HUNK1蛋白)是一种核蛋白,参与调节有丝分裂过程中的染色体动力学。睾丸特异性含溴结构域蛋白BRDT含有一个PEST序列,表明它在细胞内迅速降解。含溴结构域的蛋白质在生物体内普遍表达。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

BRD2 抗体 (G-4) 参考文献:

  1. [鼻咽癌组织中与 BRD7 相互作用的蛋白 BRD2 和 BRD3 的表达]  |  Zhou, M., et al. 2003. Ai Zheng. 22: 123-7. PMID: 12600283
  2. 在活细胞中可视化溴域蛋白对乙酰化组蛋白的选择性识别。  |  Kanno, T., et al. 2004. Mol Cell. 13: 33-43. PMID: 14731392
  3. 小鼠脊髓和背根神经节增殖和分化过程中双溴域蛋白的核-胞质定位变化  |  Crowley, T., et al. 2004. Brain Res Dev Brain Res. 149: 93-101. PMID: 15063089
  4. 在卵巢卵泡生成过程中,含溴结构域 2(Brd2)以不同的模式表达,不受 FSH 或 GDF9 作用的影响。  |  Trousdale, RK. and Wolgemuth, DJ. 2004. Mol Reprod Dev. 68: 261-8. PMID: 15112318
  5. 前绒毛特异性基因表达谱分析揭示了小鼠生殖脊在性别决定时 Ppt1 和 Brd3 基因的不同表达。  |  Boyer, A., et al. 2004. Biol Reprod. 71: 820-7. PMID: 15128596
  6. 鉴定小鼠睾丸中含溴域基因Brd2, Brd3, Brd4和Brdt的独特分化阶段特异性表达模式。  |  Shang, E., et al. 2004. Gene Expr Patterns. 4: 513-9. PMID: 15261828
  7. 对依赖于 Brd2 的细胞周期蛋白 A 转录激活的溴结构域分析。  |  Sinha, A., et al. 2005. Biochem J. 387: 257-69. PMID: 15548137
  8. 双溴结构域蛋白Brd2促进B细胞扩张和有丝分裂。  |  Belkina, AC., et al. 2014. J Leukoc Biol. 95: 451-60. PMID: 24319289
  9. BRD2与BRD3相互连接,促进Pol II转录起始和延长,从而启动细胞分化的启动子。  |  Wang, C., et al. 2022. Cell Mol Life Sci. 79: 338. PMID: 35665862
  10. BRD2染色质区室化的新机制。  |  Cheng, Y. and Wang, S. 2022. Trends Genet. 38: 1197-1198. PMID: 35811175
  11. 肝细胞癌中的血清 BRD2 自身抗体及其使用拟态肽结合 BSA 的检测方法。  |  Heo, CK., et al. 2022. Int J Oncol. 61: PMID: 36321789
  12. 与 MHC 相关的 RING3 基因的同源物 ORFX 基因的染色体定域, 基因结构和转录模式。  |  Thorpe, KL., et al. 1997. Gene. 200: 177-83. PMID: 9373153

订购信息

产品名称产品编号规格价格数量收藏夹

BRD2 抗体 (G-4)

sc-393720
200 µg/ml
RMB2377.00

BRD2 (G-4): m-IgG Fc BP-HRP 套装

sc-530556
200 µg Ab; 10 µg BP
RMB2662.00

BRD2 (G-4): m-IgGκ BP-HRP 套装

sc-524062
200 µg Ab, 40 µg BP
RMB2662.00

BRD2 (G-4): m-IgG1 BP-HRP 套装

sc-544127
200 µg Ab; 20 µg BP
RMB2662.00

BRD2 抗体 (G-4) AC

sc-393720 AC
500 µg/ml, 25% agarose
RMB3129.00

BRD2 抗体 (G-4) HRP

sc-393720 HRP
200 µg/ml
RMB2377.00

BRD2 抗体 (G-4) FITC

sc-393720 FITC
200 µg/ml
RMB2482.00

BRD2 抗体 (G-4) PE

sc-393720 PE
200 µg/ml
RMB2580.00

BRD2 抗体 (G-4) Alexa Fluor® 488

sc-393720 AF488
200 µg/ml
RMB2685.00

BRD2 抗体 (G-4) Alexa Fluor® 546

sc-393720 AF546
200 µg/ml
RMB2685.00

BRD2 抗体 (G-4) Alexa Fluor® 594

sc-393720 AF594
200 µg/ml
RMB2685.00

BRD2 抗体 (G-4) Alexa Fluor® 647

sc-393720 AF647
200 µg/ml
RMB2685.00

BRD2 抗体 (G-4) Alexa Fluor® 680

sc-393720 AF680
200 µg/ml
RMB2685.00

BRD2 抗体 (G-4) Alexa Fluor® 790

sc-393720 AF790
200 µg/ml
RMB2685.00

BRD2 (G-4) 中和勝肽

sc-393720 P
100 µg/0.5 ml
RMB511.00

What application is the blocking peptide sc-393720 P appropriate for?

Asked by: DefinitelyNotMatt
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from Good signal in Western blotThis antibody nicely detects endogenous levels of BRD2 in murine (N1E-115) and human (HepG2, U87) cancer cell lines (observed molecular weight: 90-120 kDa). Strong non-specific bands are detected at lower molecular weights, between 37 and 50 kDa.
Date published: 2021-12-12
Rated 5 out of 5 by from Great selectivityUsed this on HeLa cells to and visualize BRD2 on Westerns. Worked great and could be distinguished from BRD3 and BRD4.
Date published: 2018-04-08
Rated 1 out of 5 by from didn't work for IFNo signal in IF using 4% PFA fixed embryonic mouse brain sections( after antigen retrieval)
Date published: 2018-01-18
Rated 5 out of 5 by from Great AntibodyVery strong and specific WB signal in liver cancer cell lysates(HepG2, SNU449, Focus and Hep3B)
Date published: 2017-06-21
Rated 5 out of 5 by from Excellent antibody for Western blotting.This product has been independently tested and validated in-house at Kinexus Bioinformatics Corporation as a very potent and highly specific antibody for Western blotting with a mixture of mouse brain, testes and thymus tissue lysates. It very strongly detects an appropriately sized ~80 kDa target protein as well as an unidentified ~120 kDa cross-reactive protein in immunoblots.
Date published: 2016-10-13
Rated 5 out of 5 by from Produced positive Western blot data of BRD2Produced positive Western blot data of BRD2 expression in non-transfected and human BRD2 transfected 293T whole cell lysates. -SCBT QC
Date published: 2014-10-15
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BRD2 抗体 (G-4) is rated 4.2 out of 5 by 6.
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