Boc-D-alpha-aminobutyric acid (CAS 45121-22-0)

TEST CONDITIONS

TLC Conditions: Plate composition: Kieselgel 60, merk; Applciation 20 -100 µg; Solvent system Rf = 0.47, CHCl3: MeOH: AcOH 90: 8: 2 v/v/v, Rf = 0.92, n-Butanol: AcOH: H2O 4: 1: 1 v/v/v; Located by UV, ninhrdrin, ceric-molybdate reagentHPLC Conditions: Vydac C18, 218TP54 (4.6mm ID x 250mm L); Buffer A: 0.05% TFA in H2O, B: 0.05% TFA in MeCN; Detection 220 nm; Flow 1 ml/min; Gradient: 5% to 55% B in 50 min