Boc-3-nitro-L-tyrosine (CAS 5575-03-1)

TEST CONDITIONS

Specific Rotation (α20/D): c=1.007% in MeOHHPLC Conditions: Column: Beckman Ultrasphere 5 m, 250x4.6 mm, 150 Å; Guard Column: Supel Guard LC-18-DB, 2 cm; Retention Time: 10.050 min; Sample preparation: 2 mg/1 mL 100% Acetonitrile/Water; Injection volume: 5 l; Method: Boc; Detection at 210 nm; Gradient: 30 - 80% B, A=99.9% Water, 0.1% TFA, B=95% Acetonitrile, 4.9% Water, 0.1% TFA, 30 min, flow rate = 1.0 mL/min