Date published: 2025-9-6
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Bmx Antibody (C-4) - Western Blotting - Image 356068
Bmx 抗体 (C-4): sc-376686. Jurkat (A), HeLa (B), RAW 264. 7 (C), HUV-EC-C (D), A549 (E) 和 CCRF-CEM (F) 全细胞裂解液中 Bmx 表达的 Western 印迹分析.
Bmx 抗体 (C-4): sc-376686. Jurkat (A), HeLa (B), RAW 264. 7 (C), HUV-EC-C (D), A549 (E) 和 CCRF-CEM (F) 全细胞裂解液中 Bmx 表达的 Western 印迹分析.

Bmx 抗体 (C-4): sc-376686

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说明书
  • Bmx 抗体 C-4 是小鼠单克隆 IgG1 κ，Bmx抗体, 在3篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于human物种的Bmx的N-terminus的氨基酸2-27之间
  • Bmx 抗体 (C-4) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 Bmx; 也和以下物种反应，包括： 和 equine, canine, bovine and porcine
  • 抗Bmx抗体（C-4）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Bmx (C-4): sc-376686的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP、 1 BP-HRP">m-IgG1 BP-HRP和m-IgGκ BP-HRP是Bmx Antibody (C-4) 适用于 WB 和 IHC(P) 应用。 的首选辅助检测试剂。这些试剂现与Bmx Antibody (C-4) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

Bmx抗体（C-4）是一种IgG1 κ小鼠单克隆Bmx抗体（也称为BMX非受体酪氨酸激酶抗体、ETK抗体、X染色体上骨髓酪氨酸激酶基因蛋白抗体、上皮和内皮酪氨酸激酶抗体、细胞质酪氨酸蛋白激酶BMX抗体、X染色体上类似BTK的抗体、PSCTK3抗体、Etk/Bmx胞质酪氨酸激酶抗体或NTK38酪氨酸激酶抗体），可通过WB、IP、IF、IHC（P）和ELISA检测小鼠、大鼠和人的Bmx蛋白。Bmx抗体（C-4）既可以是非偶联的抗Bmx抗体形式，也可以是多种偶联形式的抗Bmx抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。非受体酪氨酸激酶的Tec家族由六种蛋白质组成，分别称为Tec、Emt（也称为Itk或Tsk）、Btk（以前称为Atk、BPK或Emb）、Bmx、Txk（也称为Rlk）和Dsrc28C。该家族的所有成员都含有SH3和SH2结构域，除Txk和Dsrc28C外，其氨基末端还含有Pleckstrin同源（PH）和Tec同源（TH）结构域。Tec的四种可变剪接形式在造血谱系细胞和肝细胞中广泛表达。Emt基因产物与CD28相关联，并在CD28连接后被激活。Btk对于B细胞的正常发育是必需的，而编码Btk的基因突变与患有X连锁无丙种球蛋白血症（也称为Bruton病）的家族有关。Bmx蛋白与Btk具有高度同源性，似乎在心脏中的表达水平最高。Txk的表达是T细胞特异性的，而果蝇Tec同源物Dsrc28C的表达则受到发育的调控。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Bmx 抗体 (C-4) 参考文献:

  1. IL-3 和 G-CSF 会以 PI-3K 依赖性方式激活 Bmx 酪氨酸激酶。  |  Ekman, N., et al. 2000. Oncogene. 19: 4151-8. PMID: 10962576
  2. 钠和钾调节内皮磷脂酶 C-&gamma 和 Bmx。  |  Ying, WZ., et al. 2014. Am J Physiol Renal Physiol. 307: F58-63. PMID: 24785188
  3. 抑制 PI3K/BMX 细胞存活途径可使 SCLC 对 BH3 拟态药物敏感  |  Potter, DS., et al. 2016. Mol Cancer Ther. 15: 1248-60. PMID: 27197306
  4. 目前对酪氨酸激酶 BMX 在炎症中的作用及其抑制剂的了解。  |  Qiu, L., et al. 2014. Burns Trauma. 2: 121-4. PMID: 27602372
  5. 伊布替尼抑制体外 BMX 依赖性内皮 VCAM-1 表达和体内促动脉粥样硬化内皮活化及血小板粘附。  |  Kohs, TCL., et al. 2022. Cell Mol Bioeng. 15: 231-243. PMID: 35611166
  6. 临床前球形模型确定 BMX 为转移性 MYCN 非扩增神经母细胞瘤的治疗靶点。  |  Sundaramoorthy, S., et al. 2024. JCI Insight. 9: PMID: 39133652
  7. CD28 与人 Jurkat 白血病 T 细胞系中的 Tec 家族激酶 ITK/EMT 相关，并诱导其立即酪氨酸磷酸化和活化。  |  August, A., et al. 1994. Proc Natl Acad Sci U S A. 91: 9347-51. PMID: 7524075
  8. 鉴定在 T 细胞系中主要表达的新型蛋白酪氨酸激酶 Rlk。  |  Hu, Q., et al. 1995. J Biol Chem. 270: 1928-34. PMID: 7829530
  9. TXK是一种在T细胞中表达的新型人类酪氨酸激酶，与Tec家族激酶具有相同的序列，并映射到4p12。  |  Haire, RN., et al. 1994. Hum Mol Genet. 3: 897-901. PMID: 7951233
  10. 位于染色体 Xp22.2 的 BTK/ITK/TEC/TXK 家族的新型非受体酪氨酸激酶基因 BMX。  |  Tamagnone, L., et al. 1994. Oncogene. 9: 3683-8. PMID: 7970727
  11. X 连锁丙种球蛋白血症和 X 连锁免疫缺陷基因的共定位。  |  Thomas, JD., et al. 1993. Science. 261: 355-8. PMID: 8332900
  12. 造血细胞中新型蛋白酪氨酸激酶 Emb 和 Emt 的结构和表达。  |  Yamada, N., et al. 1993. Biochem Biophys Res Commun. 192: 231-40. PMID: 8476425

订购信息

产品名称产品编号规格价格数量收藏夹

Bmx 抗体 (C-4)

sc-376686
200 µg/ml
RMB2377.00

Bmx (C-4): m-IgG Fc BP-HRP 套装

sc-529923
200 µg Ab; 10 µg BP
RMB2662.00

Bmx (C-4): m-IgGκ BP-HRP 套装

sc-523046
200 µg Ab, 40 µg BP
RMB2662.00

Bmx (C-4): m-IgG1 BP-HRP 套装

sc-543768
200 µg Ab; 20 µg BP
RMB2662.00

Bmx 抗体 (C-4) AC

sc-376686 AC
500 µg/ml, 25% agarose
RMB3129.00

Bmx 抗体 (C-4) HRP

sc-376686 HRP
200 µg/ml
RMB2377.00

Bmx 抗体 (C-4) FITC

sc-376686 FITC
200 µg/ml
RMB2482.00

Bmx 抗体 (C-4) PE

sc-376686 PE
200 µg/ml
RMB2580.00

Bmx 抗体 (C-4) Alexa Fluor® 488

sc-376686 AF488
200 µg/ml
RMB2685.00

Bmx 抗体 (C-4) Alexa Fluor® 546

sc-376686 AF546
200 µg/ml
RMB2685.00

Bmx 抗体 (C-4) Alexa Fluor® 594

sc-376686 AF594
200 µg/ml
RMB2685.00

Bmx 抗体 (C-4) Alexa Fluor® 647

sc-376686 AF647
200 µg/ml
RMB2685.00

Bmx 抗体 (C-4) Alexa Fluor® 680

sc-376686 AF680
200 µg/ml
RMB2685.00

Bmx 抗体 (C-4) Alexa Fluor® 790

sc-376686 AF790
200 µg/ml
RMB2685.00

Bmx (C-4) 中和勝肽

sc-376686 P
100 µg/0.5 ml
RMB511.00

Hi SC, what is the recommended dilution of sc-376686 for flow cytometry?

Asked by: Flow_BMX
Thank you for your question. We recommend using Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 direct conjugate of Bmx Antibody (C-4): sc-376686 for immunofluorescence. The recommended amount of antibody is 1 µg of antibody per 1x10^6 cells.
Answered by: Tech Support Europe
Date published: 2019-07-17

What application is the blocking peptide sc-376686 P appropriate for?

Asked by: Randy McDonald
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from Good results!Produces clean, strong bands. Works really well for bone marrow cells.
Date published: 2019-04-12
Rated 5 out of 5 by from Good antibodyI used it on my human samples, I got nice result, very good antibody for WB, with BSA as blocking, 1/1000 df
Date published: 2017-03-20
Rated 5 out of 5 by from Well characterized Bmx antibodyProduced strong, clean bands in WB analysis of Bmx in HeLa, RAW 264.7, HUV-EC-C, A549 and CCRF-CEM whole cell lysates.
Date published: 2017-02-08
Rated 5 out of 5 by from Better than H-220I used 0.2ug/ml dilution in BSA. The antibody worked great! This antibody does not detect all the isoforms of Etk/BMX. Produced clean bands without detectable background!
Date published: 2016-11-14
Rated 5 out of 5 by from Highly potent and specific antibodyThis monoclonal antibody was independently tested in-house by Kinexus Bioinformatics Corporation and validated for use for Western blotting. There was extremely strong detection of the ~85 kDa target protein in mouse tissues (brain/testes/thymus/muscle mix). No major cross-reactivities were observed at 2 µg/ml antibody concentration with off-target proteins in the mouse tissues tested when a high dilution of the antibody was used.
Date published: 2016-11-10
Rated 5 out of 5 by from Produced positive Western Blot data of BmxProduced positive Western Blot data of Bmx expression in non-transfected and human Bmx transfected 293T whole cell lysates. -SCBT QC
Date published: 2015-05-08
Rated 5 out of 5 by from Produced positive Western Blot data of BmxProduced positive Western Blot data of Bmx expression in Jurkat whole cell lysate. -SCBT QC
Date published: 2013-05-23
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Bmx 抗体 (C-4) is rated 4.9 out of 5 by 7.
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