Date published: 2025-9-7
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Bim Antibody (H-5) - Western Blotting - Image 386093
Bim 抗体 (H-5) HRP: sc-374358 HRP. 直接 Western 印迹分析 HuT 78 (A) 和 Daudi (B) 全细胞裂解液中 Bim 的表达.
Bim 抗体 (H-5) HRP: sc-374358 HRP. 直接 Western 印迹分析 HuT 78 (A) 和 Daudi (B) 全细胞裂解液中 Bim 的表达.

Bim 抗体 (H-5): sc-374358

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说明书
  • Bim 抗体 H-5 是小鼠单克隆 IgG1 κ，Bim抗体, 在47篇文献中引用，规格为200 µg/ml
  • 免疫human物种的BimEL的氨基酸4-195
  • Bim 抗体 (H-5) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 BimEL, BimL and BimS
  • 抗Bim抗体（H-5）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Bim (H-5): sc-374358的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP是Bim Antibody (H-5) 适用于 WB 应用。 的首选辅助检测试剂。该试剂现与Bim Antibody (H-5) 搭配使用（请参阅下面的订购信息）。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

此外 Bim 抗体包括 BimEL

Bim 抗体（H-5）是一种 IgG1 κ 小鼠单克隆 Bim 抗体（也称为 BCL2L11 抗体），可通过 WB、IP、IF 和 ELISA 检测小鼠、大鼠和人类的 Bim 蛋白。Bim 抗体（H-5）既可以是非偶联的抗 Bim 抗体形式，也可以是多种偶联形式的抗 Bim 抗体，包括琼脂糖、HRP、PE、FITC 和多种 Alexa Fluor® 偶联物。促凋亡 Bcl-2 家族成员通过中和其抗凋亡的相关物来促进细胞死亡，否则这些相关物会通过调节 caspase 活性来维持细胞活力。Bim 属于 Bcl-2 相关蛋白的仅含 BH3 亚组，并以三种不同的异构体形式存在：BimS（短型）、BimL（长型）和 BimEL（超长型）。ERK1/2 会磷酸化 BimEL，导致该异构体通过蛋白酶体途径快速降解。BimEL 上至少存在三个 ERK1/2 磷酸化位点，而 ERK1/2 不影响 BimS 和 BimL，这暗示 BimEL 在细胞生存信号传导中具有独特作用。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Bim 抗体 (H-5) 参考文献:

  1. 促进细胞凋亡的纯 BH3 蛋白 bim 在造血细胞, 上皮细胞, 神经细胞和生殖细胞中都有表达。  |  O'Reilly, LA., et al. 2000. Am J Pathol. 157: 449-61. PMID: 10934149
  2. 淋巴细胞中促凋亡蛋白 Bim 的磷酸化与防止细胞凋亡有关。  |  Seward, RJ., et al. 2003. Mol Immunol. 39: 983-93. PMID: 12749905
  3. Erk1/2 对 Bim-EL 的丝氨酸 69 进行磷酸化，促进其通过蛋白酶体途径降解，并调节其促凋亡功能。  |  Luciano, F., et al. 2003. Oncogene. 22: 6785-93. PMID: 14555991
  4. 细胞外信号调节激酶 1/2是受血清刺激的 'Bim(EL)激酶'，它与纯 BH3 蛋白 Bim(EL) 结合，导致其磷酸化和周转。  |  Ley, R., et al. 2004. J Biol Chem. 279: 8837-47. PMID: 14681225
  5. Bim 在正常和 Bcr-Abl 表达造血祖细胞凋亡中的作用  |  Kuribara, R., et al. 2004. Mol Cell Biol. 24: 6172-83. PMID: 15226421
  6. Bim 是一种凋亡传感器，能对表皮生长因子传递的存活信号的缺失做出反应，但不能对整合素提供的存活信号做出反应。  |  Wang, P., et al. 2004. J Biol Chem. 279: 41280-5. PMID: 15292207
  7. Caspases 对 Mcl-1 的裂解削弱了其对抗 Bim 诱导的细胞凋亡的能力。  |  Herrant, M., et al. 2004. Oncogene. 23: 7863-73. PMID: 15378010
  8. Bim 和 Mcl-1 表达的不平衡控制着人类骨髓瘤细胞的存活。  |  Gomez-Bougie, P., et al. 2004. Eur J Immunol. 34: 3156-64. PMID: 15459900
  9. 存活因子诱导的细胞外信号调节激酶使 BIM 磷酸化，抑制其与 BAX 的缔合作用和促凋亡活性。  |  Harada, H., et al. 2004. Proc Natl Acad Sci U S A. 101: 15313-7. PMID: 15486085
  10. 建筑信息模型（BIM）中的生成式设计：方法和要求。  |  Ma, W., et al. 2021. Sensors (Basel). 21: PMID: 34450882
  11. MCL-1/BIM蛋白复合物中的信号传播。  |  Heckmeier, PJ., et al. 2022. J Mol Biol. 434: 167499. PMID: 35189130
  12. BCL2L11诱导介导甲状腺癌对Src和MEK1/2抑制的敏感性。  |  Rose, MM., et al. 2023. Cancers (Basel). 15: PMID: 36672327

订购信息

产品名称产品编号规格价格数量收藏夹

Bim 抗体 (H-5)

sc-374358
200 µg/ml
RMB2377.00

Bim (H-5): m-IgG Fc BP-HRP 套装

sc-525957
200 µg Ab; 10 µg BP
RMB2662.00

Bim 抗体 (H-5) AC

sc-374358 AC
500 µg/ml, 25% agarose
RMB3129.00

Bim 抗体 (H-5) HRP

sc-374358 HRP
200 µg/ml
RMB2377.00

Bim 抗体 (H-5) FITC

sc-374358 FITC
200 µg/ml
RMB2482.00

Bim 抗体 (H-5) PE

sc-374358 PE
200 µg/ml
RMB2580.00

Bim 抗体 (H-5) Alexa Fluor® 488

sc-374358 AF488
200 µg/ml
RMB2685.00

Bim 抗体 (H-5) Alexa Fluor® 546

sc-374358 AF546
200 µg/ml
RMB2685.00

Bim 抗体 (H-5) Alexa Fluor® 594

sc-374358 AF594
200 µg/ml
RMB2685.00

Bim 抗体 (H-5) Alexa Fluor® 647

sc-374358 AF647
200 µg/ml
RMB2685.00

Bim 抗体 (H-5) Alexa Fluor® 680

sc-374358 AF680
200 µg/ml
RMB2685.00

Bim 抗体 (H-5) Alexa Fluor® 790

sc-374358 AF790
200 µg/ml
RMB2685.00

How much ug of whole cell lysate would be suggested to run on western to detect BIM (h-5) using this antibody

Asked by: md42000
Thank you for your question. We recommend the following amounts of protein for any western blotting experiment: 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane.
Answered by: Tech Support Europe
Date published: 2022-04-28

Can I use this antibody to detect rat bim?

Asked by: TheHeroZeus
Thank you for your question. We recommend using Anti-Bim Antibody (H-5): sc-374358 with rat samples. We guarantee it to work when following our protocol. For additional questions please contact our European Support office. You may reach them toll-free by phone at 00800 4573 8000, by email at [email protected], or contact them by live chat on our website at www.scbt.com.
Answered by: Blake J
Date published: 2021-07-12

can we get custom formulation in PBS and career free in bulk amounts?

Asked by: Raj Ti
Thank you for your question. Yes, we can offer these custom formulations and bulk quantities for this antibody. Please contact our Special Orders Department to inquire about availability and to receive a quote. Please call our Technical Service Department, or email <[email protected]>.
Answered by: Technical Support
Date published: 2018-12-19

Are there any issues with background when using this antibody to stain mouse tissue or cells?

Asked by: AbPolly
Thank you for your question. This antibody can be used to stain mouse tissue and cells. In this case, we recommend using a directly conjugated mouse monoclonal primary antibody. This antibody is currently available conjugated to HRP, PE, FITC, AF488 and AF647.
Answered by: Technical Support 10
Date published: 2017-01-11

Can sc-374358 AC be used in Western Blot?

Asked by: AbPolly
Thank you for your question. The agarose conjugated version of this Bim antibody, sc-374358 AC, is only recommended for IP or other applications where agarose is necessary. It is not appropriate for use in Western Blot.
Answered by: Technical Support 10
Date published: 2017-01-11
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Rated 3 out of 5 by from It works with western blotThe antibody can detect a specific band with 1:250 dilution for western blot, but signal is weaker. Samples were HeLa cell extract.
Date published: 2019-10-08
Rated 4 out of 5 by from General effectA brand with Poor clarity was detected in wb in QBC cell line
Date published: 2017-10-15
Rated 3 out of 5 by from Bim not detected in COV434 cellsAnticorps utilisé pour détecter Bim à partir d'extraits protéiques de cellules COV434. Détection d'une bande aspécifique mais pas de bande au poids moléculaire attendu après une exposition de 30 minutes.
Date published: 2017-10-11
Rated 5 out of 5 by from very goodI tested it in C42B and VCaP cell lines. It works very good.
Date published: 2017-09-27
Rated 4 out of 5 by from 条带清晰上周申请了BIM，今天显影发光，条带略妥，建议新鲜提的蛋白马上跑蛋白，这样会好一些，如果是冻存的话会比较弱
Date published: 2017-09-19
Rated 1 out of 5 by from The sample we received did not workAt least the sample we received did not work. It is strange we have received several samples not working. Usually the same abs we purchased work.
Date published: 2017-09-16
Rated 2 out of 5 by from 条带很弱一周前拿到此产品，在293T细胞上进行内源性检测，Western Blot结果显示条带大小正确，但是不清晰
Date published: 2017-09-01
Rated 5 out of 5 by from Good results in western blot using U138 cell lineVery clear WB data, with strong signal and little background
Date published: 2017-07-28
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Bim 抗体 (H-5) is rated 4.2 out of 5 by 17.
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