Date published: 2026-10-24

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BID 抗体 (E-7): sc-514622

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说明书
  • BID抗体(E-7)是小鼠单克隆IgG2a κ, 在8篇文献中引用,规格为200 µg/ml
  • 特异性抗原位于human物种的BID的C-terminus的氨基酸172-195之间
  • 抗-BID 抗体 (E-7) 推荐用于 WB, IP, IF 和 ELISA,检测mouse, rat 和human 来源的 BID
  • 抗BID抗体(E-7)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • m-IgG Fc BP-HRP和 2a BP-HRP">m-IgG2a BP-HRP是BID Antibody (E-7) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与BID Antibody (E-7) 打包提供(请参阅下面的订购信息)。
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関連項目

BID抗体(E-7)是一种小鼠单克隆IgG2a kappa轻链抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)和酶联免疫吸附测定(ELISA)等方法检测小鼠、大鼠和人类样本中的BID。抗BID抗体(E-7)有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶(HRP)、藻红蛋白(PE)、异硫氰酸荧光素(FITC)和多种Alexa Fluor®结合物。BID在细胞凋亡调节中起着关键作用,是Bcl-2家族中促进细胞凋亡的成员。BID包含一个BH3结构域,能够与Bcl-2和Bcl-x等抗凋亡蛋白相互作用,有效抵消其抑制死亡的作用。这种相互作用对于凋亡信号通路至关重要,因为BID有助于促进细胞死亡,以应对各种应激信号,从而维持细胞内环境平衡,防止细胞无序增殖。BID主要定位于细胞质中,但也可以在膜部分找到,在肾脏中的表达水平很高,在脑、脾、肝、睾丸和肺中也有可检测到的水平。BID能够与Bax等其他蛋白形成异二聚体,这进一步强调了BID在细胞存活和死亡的复杂平衡中的重要性,使得BID(E-7)单克隆抗体成为研究细胞凋亡和相关细胞过程的研究人员的重要工具。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

BID 抗体 (E-7) 参考文献:

  1. Bid诱导的Bax构象变化是细胞凋亡过程中线粒体细胞色素c释放的原因。  |  Desagher, S., et al. 1999. J Cell Biol. 144: 891-901. PMID: 10085289
  2. Bid缺陷小鼠对Fas诱导的肝细胞凋亡具有抗性。  |  Yin, XM., et al. 1999. Nature. 400: 886-91. PMID: 10476969
  3. BID介导缺氧/缺糖和脑局部缺血后的神经细胞死亡。  |  Plesnila, N., et al. 2001. Proc Natl Acad Sci U S A. 98: 15318-23. PMID: 11742085
  4. 在死亡受体结合后,Bid激活原代肝细胞中的多种线粒体凋亡机制。  |  Zhao, Y., et al. 2003. Gastroenterology. 125: 854-67. PMID: 12949730
  5. 在诱导嗜酸性粒细胞的外源性和内源性凋亡过程中激活Bid。  |  Segal, M., et al. 2007. Immunol Cell Biol. 85: 518-24. PMID: 17549073
  6. Bid在肝癌细胞中表现出S期检查点激活,并在应对依托泊苷诱导的DNA损伤时发挥促凋亡作用。  |  Song, G., et al. 2008. Apoptosis. 13: 693-701. PMID: 18373075
  7. Bid参与HeLa细胞基因毒性药物诱导的细胞凋亡,对于死亡受体配体的凋亡和协同效应至关重要。  |  Köhler, B., et al. 2008. PLoS One. 3: e2844. PMID: 18665234
  8. 在γ射线照射的受体中,Bid是供体来源淋巴细胞再生的正调控因子。  |  Shen, H., et al. 2011. Exp Hematol. 39: 947-957.e1. PMID: 21703985
  9. BID在组织学海马切片培养中介导缺氧缺糖诱导的神经元损伤,并在瞬态局灶性脑缺血模型中调节组织炎症,且不会改变病变体积。  |  Martin, NA., et al. 2016. Front Cell Neurosci. 10: 14. PMID: 26869884
  10. BID促进饮食诱导的肥胖和代谢综合征与肠道微生物群有关。  |  Yan, S., et al. 2022. Hepatol Commun. 6: 3349-3362. PMID: 36382356

订购信息

产品名称产品编号规格价格数量收藏夹

BID 抗体 (E-7)

sc-514622
200 µg/ml
CNY2422.00

BID (E-7): m-IgG Fc BP-HRP 套装

sc-526383
200 µg Ab; 10 µg BP
CNY2715.00

BID (E-7): m-IgG2a BP-HRP 套装

sc-546614
200 µg Ab; 10 µg BP
CNY2715.00

BID 抗体 (E-7) AC

sc-514622 AC
500 µg/ml, 25% agarose
CNY3189.00

BID 抗体 (E-7) HRP

sc-514622 HRP
200 µg/ml
CNY2422.00

BID 抗体 (E-7) FITC

sc-514622 FITC
200 µg/ml
CNY2527.00

BID 抗体 (E-7) PE

sc-514622 PE
200 µg/ml
CNY2625.00

BID 抗体 (E-7) Alexa Fluor® 488

sc-514622 AF488
200 µg/ml
CNY2738.00

BID 抗体 (E-7) Alexa Fluor® 546

sc-514622 AF546
200 µg/ml
CNY2738.00

BID 抗体 (E-7) Alexa Fluor® 594

sc-514622 AF594
200 µg/ml
CNY2738.00

BID 抗体 (E-7) Alexa Fluor® 647

sc-514622 AF647
200 µg/ml
CNY2738.00

BID 抗体 (E-7) Alexa Fluor® 680

sc-514622 AF680
200 µg/ml
CNY2738.00

BID 抗体 (E-7) Alexa Fluor® 790

sc-514622 AF790
200 µg/ml
CNY2738.00

BID (E-7) 中和勝肽

sc-514622 P
100 µg/0.5 ml
CNY519.00

Is the Alexa-Fluor conjugate of this AB available for flow cytometry experiments as stated in the description or is it not, since the unconjugated AB is just recommended for WB, IP, IF & ELISA?

Asked by: Fest
Thank you for your question. We do not test all of our antibodies in flow cytometry. We recommend and warrant the Alexa Fluor® 488, 546, 594, and 647 conjugates of BID (E-7): sc-514622 for use in flow cytometry. You can find our recommended protocol for flow cytometry by following this link: https://www.scbt.com/scbt/resources/protocols/flow-cytometry
Answered by: SCBT Heidelberg Support
Date published: 2018-12-22

What application is the blocking peptide sc-514622 P appropriate for?

Asked by: Professor Griffin
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-03-01
  • y_2026, m_10, d_24, h_5CST
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Rated 5 out of 5 by kuanlab from western blotThe antibody can gave a nice clean band. It is a good antibody.
Date published: 2023-03-10
Rated 4 out of 5 by Silke87 from Worked well!I used the antibody at a dilution of 1:500 in 5% milk/TBST and an incubation over the weekend for murin B16 melanoma cells and it worked quited specific.
Date published: 2018-02-26
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BID 抗体 (E-7) is rated 4.5 out of 5 by 2.
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