Date published: 2025-9-7

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β-dystroglycan 抗体 (56): sc-135891

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说明书
  • beta-dystroglycan 抗体 56 是小鼠单克隆 IgG2a,提供 50 µg/0.5 ml
  • 免疫human物种的β-dystroglycan的氨基酸655-767
  • 推荐用于 human 来源的β-dystroglycan WB 和 IP检测
  • 看 β-dystroglycan (4F7): sc-33702,可查看有标记的 β-dystroglycan 抗体,包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • 目前,我们还没有完成β-dystroglycan 抗体 (56)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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    関連項目

    Dystroglycan(DG)是几种细胞外基质分子(包括层粘连蛋白、聚集蛋白和 perlecan)的细胞表面受体。Dystroglycan的功能对于早期发育中基底膜的形成和细胞表面层粘连蛋白的组织是必需的。α-dystroglycan是一种膜相关的细胞外糖蛋白,通过与跨膜糖蛋白β-dystroglycan结合锚定在细胞膜上,形成α/β-dystroglycan复合物。此外,dystroglycan是多分子复合物的一部分,它在肌膜上与dystrophin结合,形成dystrophin相关蛋白复合物,或在神经肌肉接头处与utrophin结合,形成utrophin相关蛋白复合物。还认为dystroglycan参与神经肌肉接头处烟碱型乙酰胆碱受体的聚集。

    仅限研究使用。不适用于诊断和治疗用途。

    Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

    LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

    β-dystroglycan 抗体 (56) 参考文献:

    1. 肌营养不良蛋白与β-肌营养不良聚糖的相互作用受酪氨酸磷酸化的调节。  |  Ilsley, JL., et al. 2001. Cell Signal. 13: 625-32. PMID: 11495720
    2. 鉴定α-肌营养不良蛋白 C 终止子区域内的β-肌营养不良蛋白结合表位。  |  Sciandra, F., et al. 2001. Eur J Biochem. 268: 4590-7. PMID: 11502221
    3. ezrin介导的细胞骨架重组促进了β-肌冻蛋白的核导入。  |  Vásquez-Limeta, A., et al. 2014. PLoS One. 9: e90629. PMID: 24599031
    4. α-和β-缓激肽的缺失与沃克-瓦尔堡综合征有关。  |  Riemersma, M., et al. 2015. Neurology. 84: 2177-82. PMID: 25934851
    5. 控制核β-岩藻糖含量对于维持核包膜的完整性和功能至关重要。  |  Vélez-Aguilera, G., et al. 2018. Biochim Biophys Acta Mol Cell Res. 1865: 406-420. PMID: 29175376
    6. 作为 GMPPB 基因相关肌营养不良症标志物的 beta-dystroglycan 的迁移率变化。  |  Sarkozy, A., et al. 2018. J Neurol Neurosurg Psychiatry. 89: 762-768. PMID: 29437916
    7. β-Dystroglycan-Emerin相互作用的分子基础和生物学意义。  |  Gómez-Monsivais, WL., et al. 2020. Int J Mol Sci. 21: PMID: 32824881
    8. 过敏性哮喘患者气道平滑肌中的β-Dystroglycan与嗜酸性粒细胞之间的联系  |  Shareef, SH., et al. 2021. Inflammation. 44: 1060-1068. PMID: 33566255
    9. β-肌营养不良糖上肌营养不良蛋白锚定位点的鉴定和表征。  |  Jung, D., et al. 1995. J Biol Chem. 270: 27305-10. PMID: 7592992
    10. 与 beta-Dystroglycan 缺乏症有关的肌肉萎缩症。  |  Salih, MA., et al. 1996. Ann Neurol. 40: 925-8. PMID: 9007100

    订购信息

    产品名称产品编号规格价格数量收藏夹

    β-dystroglycan 抗体 (56)

    sc-135891
    50 µg/0.5 ml
    $316.00

    Does this antibody (β-dystroglycan Antibody (56): sc-135891) recognize native or only denature beta-dystroglycan on Western blot, or it can be used for frozen section immunofluorescent staining or non-denature cell lysate for immunoprecipitation? Thanks

    Asked by: John63
    Thank you for your question. Based on our tests with this antibody we do not recommend it for immunofluorescence. It has been shown to work well in SDS-PAGE western blots and IP.
    Answered by: Tech Support Europe
    Date published: 2021-01-25

    I am using β-dystroglycan (56): sc-135891 antibody for immunoprecipitation, how much antibody should I use?

    Asked by: Randy McDonald
    Thank you for your question. We recommend using 1–10 µl (i.e., 0.2–2 µg) of antibody with 1 ml of cell lysate. However, optimal antibody concentration should be determined by titration. Please find our full immunoprecipitation protocol here: https://www.scbt.com/scbt/resources/protocols/immunoprecipitation-western-blots
    Answered by: Technical Support
    Date published: 2017-03-24
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    Rated 4 out of 5 by from Very strong band of interest obtained inVery strong band of interest obtained in WB in JAR whole cell lysate. -SCBT QC
    Date published: 2015-01-24
    Rated 4 out of 5 by from Antibody performed well in WB in NCIAntibody performed well in WB in NCI-H929, ARPE-19, SKBR-3 and JEG-3 whole cell lysates. -SCBT QC
    Date published: 2013-10-25
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    β-dystroglycan 抗体 (56) is rated 4.0 out of 5 by 2.
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