Date published: 2025-9-8
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β-Amyloid Antibody (B-4) - Western Blotting - Image 354510
β-淀粉样蛋白抗体 (B-4): sc-28365. HeLa (A) 和 MCF7 (B) 全细胞裂解液中 β 淀粉样蛋白表达的 Western 印迹分析.
β-淀粉样蛋白抗体 (B-4): sc-28365. HeLa (A) 和 MCF7 (B) 全细胞裂解液中 β 淀粉样蛋白表达的 Western 印迹分析.

β-Amyloid 抗体 (B-4): sc-28365

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说明书
  • beta-Amyloid 抗体 B-4 是小鼠单克隆 IgG2a κ，β-Amyloid抗体, 在135篇文献中引用，规格为200 µg/ml
  • 免疫human物种的β-Amyloid的氨基酸672-714
  • beta-Amyloid 抗体 (B-4) 推荐用于WB, IP, IF, IHC(P) 和 ELISA ，检测 mouse, rat, human 和 来源的β-Amyloid and Amyloid A4; 也和以下物种反应，包括： 和 equine, canine, bovine, porcine and avian
  • 抗beta-Amyloid抗体（B-4）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取β-Amyloid (B-4): sc-28365的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG2a BP-HRP 是 β-Amyloid 抗体 (B-4) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与β-Amyloid 抗体 (B-4) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

此外 Amyloid 抗体 包括 β-Amyloid

β-淀粉样蛋白抗体（B-4）是一种小鼠单克隆IgG2a kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、 免疫荧光（IF）、免疫组织化学和酶联免疫吸附测定（ELISA）检测小鼠、大鼠和人类来源的β-淀粉样蛋白。β-淀粉样蛋白（B-4）抗体有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。β-淀粉样蛋白由淀粉样蛋白前体蛋白（APP）的蛋白水解作用产生，在阿尔茨海默氏病的发病机理中起着关键作用，因为淀粉样蛋白的积累会导致大脑中神经毒性斑块的形成。这些斑块与神经退行性和认知能力下降有关，因此对β-淀粉样蛋白的研究对于了解和潜在治疗阿尔茨海默氏病及相关疾病至关重要。β-淀粉样蛋白与其他蛋白（如APLP1和APLP2）的相互作用在细胞信号传导和膜动力学中具有重要意义，因为这些蛋白与APP在结构上相似。APLP2是一种膜结合蛋白，与APP的跨膜-细胞质结构域具有同源性，进一步强调了这些蛋白在神经退行性疾病中的相互联系。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

β-Amyloid 抗体 (B-4) 参考文献:

  1. 阿尔茨海默病：细胞生物学视角。  |  Kosik, KS. 1992. Science. 256: 780-3. PMID: 1589757
  2. 抑制糖磷脂的生物合成可减少β-淀粉样前体蛋白和淀粉样β-肽的分泌。  |  Tamboli, IY., et al. 2005. J Biol Chem. 280: 28110-7. PMID: 15923191
  3. β-淀粉样蛋白与线粒体动态之间的关系。  |  Kim, DI., et al. 2017. Cell Mol Neurobiol. 37: 955-968. PMID: 27766447
  4. β-淀粉样蛋白低聚物的毒性和多态性。  |  Huang, YR. and Liu, RT. 2020. Int J Mol Sci. 21: PMID: 32599696
  5. 跨β淀粉样蛋白组装体作为复杂的催化机制。  |  Pal, S., et al. 2021. Chem Commun (Camb). 57: 7597-7609. PMID: 34278403
  6. β-淀粉样蛋白和α-突触核蛋白的共组装形成的低聚物。  |  Kim, JR. 2023. Front Mol Biosci. 10: 1153839. PMID: 37021111
  7. 通过电场破坏二聚体β-淀粉样蛋白。  |  Vargas-Rosales, PA., et al. 2023. ACS Phys Chem Au. 3: 456-466. PMID: 37780539
  8. 与阿尔茨海默病和脑淀粉样血管病有关的淀粉样 beta/A4 肽。  |  Hirai, S. and Okamoto, K. 1993. Intern Med. 32: 923-5. PMID: 8204971
  9. 从淀粉样蛋白前体及其片段中生成 beta A4。  |  Dyrks, T., et al. 1993. FEBS Lett. 335: 89-93. PMID: 8243673
  10. 磷酸酶 1 和 2A 的慢性抑制作用诱发大鼠 tau 的成对螺旋丝状磷酸化, β/A4-淀粉样蛋白沉积和记忆损伤。  |  Arendt, T., et al. 1995. Neuroscience. 69: 691-8. PMID: 8596639
  11. 淀粉样变性：诊断和治疗最新进展回顾。  |  Gillmore, JD., et al. 1997. Br J Haematol. 99: 245-56. PMID: 9375734
  12. 阿尔茨海默氏症和唐氏综合症患者体内β淀粉样前体蛋白和泛素-B的框架突变体。  |  van Leeuwen, FW., et al. 1998. Science. 279: 242-7. PMID: 9422699

订购信息

产品名称产品编号规格价格数量收藏夹

β-Amyloid 抗体 (B-4)

sc-28365
200 µg/ml
RMB2377.00

β-Amyloid (B-4): m-IgG Fc BP-HRP 套装

sc-525527
200 µg Ab; 10 µg BP
RMB2662.00

β-Amyloid (B-4): m-IgG2a BP-HRP 套装

sc-546305
200 µg Ab; 10 µg BP
RMB2662.00

β-Amyloid 抗体 (B-4) AC

sc-28365 AC
500 µg/ml, 25% agarose
RMB3129.00

β-Amyloid 抗体 (B-4) HRP

sc-28365 HRP
200 µg/ml
RMB2377.00

β-Amyloid 抗体 (B-4) FITC

sc-28365 FITC
200 µg/ml
RMB2482.00

β-Amyloid 抗体 (B-4) PE

sc-28365 PE
200 µg/ml
RMB2580.00

β-Amyloid 抗体 (B-4) Alexa Fluor® 488

sc-28365 AF488
200 µg/ml
RMB2685.00

β-Amyloid 抗体 (B-4) Alexa Fluor® 546

sc-28365 AF546
200 µg/ml
RMB2685.00

β-Amyloid 抗体 (B-4) Alexa Fluor® 594

sc-28365 AF594
200 µg/ml
RMB2685.00

β-Amyloid 抗体 (B-4) Alexa Fluor® 647

sc-28365 AF647
200 µg/ml
RMB2685.00

β-Amyloid 抗体 (B-4) Alexa Fluor® 680

sc-28365 AF680
200 µg/ml
RMB2685.00

β-Amyloid 抗体 (B-4) Alexa Fluor® 790

sc-28365 AF790
200 µg/ml
RMB2685.00

I am a beginner in WB studies..so could you please let me know the difference between beta amyloid antibody D-11 and B-4 as I am confused for ordering an A-beta antibody.My samples are of mice and I need to study the expression of A-beta.

Asked by: Anjii
Thank you for your question. The "(D-11)" and "(B-4)" are alternate clone names for different antibodies that both detect the same β-Amyloid protein. Clone names are used to distinguish between antibodies and are used as unique identifiers. Both of these antibodies are suitable for detection of β-Amyloid protein in mouse samples. β-Amyloid Antibody (B-4): sc-28365 is recommended for detection of β-Amyloid and Amyloid A4 of mouse, rat, human and avian origin by WB, IP, IF, IHC(P) and ELISA. This is the more popular choice between these two antibodies, based on the number or product citations.
Answered by: Technical Support
Date published: 2018-12-05

Can I use this mouse monoclonal antibody for immunohistochemical staining of mouse tissue? Or even double or triple staining on the same samples?

Asked by: DefinitelyNotMatt
Thank you for your question. Yes. We recommend using a directly conjugated mouse monoclonal primary antibody. This antibody is currently available conjugated to phycoerythrin (sc-28365 PE), fluorescein (sc-28365 FITC), Alexa Fluor® 488 (sc-28365 AF488) or Alexa Fluor® 647 (sc-28365 AF647). Please contact Technical Service if you have further questions concerning this product.
Answered by: Technical Service 11
Date published: 2017-01-10
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Rated 5 out of 5 by from Perfect Western BlotI have used these antibodies to detect AB in neurons, the 1:500 dilution works good
Date published: 2022-02-17
Rated 5 out of 5 by from Great bandDilution 1:1000 and I got great bands around 100kDA.
Date published: 2019-12-03
Rated 4 out of 5 by from Good bandsNice bands around 100 kda, using1:1250 dilution with superblock at 4 degree overnight
Date published: 2019-10-28
Rated 5 out of 5 by from Western blot of β-amyloid peptide in rat brainGood Western blot data of β-amyloid peptide expression in rat hippocampal brain tissue. Specific band at 1:200 dilution.
Date published: 2019-08-16
Rated 5 out of 5 by from very good signal in WBWB: 1:1000 MDA cell lysate two clear band at 100 and 45 kDa
Date published: 2019-08-02
Rated 5 out of 5 by from Good Western Blot specificityWe used the β-Amyloid Antibody in rat brain tissue (hippocampus and cortex samples). We tested 50 and 75 μg of protein. The antibody had a great performance in every condition tested, using membrane visualisation with Super Signal West Pico PLUS Chemiluminescent substrate but also home reagents, on a Chemidoc Image Station.
Date published: 2018-12-20
Rated 5 out of 5 by from good specificity in WBit shows good specificity in lung cancer cell line protein sample
Date published: 2017-11-16
Rated 5 out of 5 by from We feel good about the antibodyWe can detect a strong signal band in mouse brain tissue. The antibody has good immunoblotting effect.
Date published: 2017-11-02
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β-Amyloid 抗体 (B-4) is rated 4.7 out of 5 by 33.
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