Date published: 2025-9-6

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beta Actin 抗体 (ACTBD11B7): sc-81178

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说明书
  • beta Actin抗体(ACTBD11B7)是小鼠单克隆IgG1 κ, 在1,080篇文献中引用,规格为100 µg/ml
  • 针对与human β-Actin C-terminus附近区域相对应的重组蛋白
  • 推荐用于 mouse, rat 和 human 来源的β-Actin WB, IP, IF 和 FCM检测
  • 看 beta Actin (C4): sc-47778,可查看有标记的 beta Actin 抗体,包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • 关于如何获取beta Actin (ACTBD11B7): sc-81178免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP1 BP-HRP">m-IgG1 BP-HRP是beta Actin Antibody (ACTBD11B7) for WB applications. 的首选辅助检测试剂,这些试剂现与beta Actin Antibody (ACTBD11B7) 打包提供(请参阅下面的订购信息)。

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関連項目

β-肌动蛋白抗体(ACTBD11B7)是一种小鼠单克隆IgG1κ轻链抗体,针对与人类β-肌动蛋白C末端附近区域相对应的重组蛋白。具体来说,抗-β-肌动蛋白抗体(ACTBD11B7)可检测小鼠、大鼠和人类的β-肌动蛋白,适用于包括蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)和流式细胞术(FCM)在内的多种应用。β-肌动蛋白是一种高度保守的细胞质蛋白,作为细胞骨架的基本组成部分,在维持细胞结构、运动和完整性方面发挥着至关重要的作用。β-肌动蛋白参与细胞分裂、囊泡和细胞器运动、细胞信号传导以及细胞连接和形状的形成与维持等各种细胞过程。β-肌动蛋白的动态组织受小GTP酶(如Rho、Rac和Cdc42)的调节,这些小GTP酶分别控制肌动蛋白应力纤维、膜褶皱和纤毛的组装。这些相互作用对于细胞骨架重组和细胞对环境信号的反应至关重要。β-肌动蛋白与多种蛋白相互作用,包括肌球蛋白马达蛋白和Arp2/3复合物的成分,促进肌动蛋白丝的成核和运动。β-肌动蛋白在所有真核细胞中普遍表达,与γ-肌动蛋白一起构成非肌肉组织中的主要肌动蛋白同工型,而α-肌动蛋白主要存在于肌肉细胞中。鉴于β-肌动蛋白的重要功能和恒定的表达水平,它可用作蛋白质检测中的加载对照,以确保样品之间的蛋白质加载量相等。因此,抗β-肌动蛋白抗体(ACTBD11B7)成为研究细胞骨架动力学、细胞运动和相关信号传导通路的研究人员的宝贵工具。

仅限研究使用。不适用于诊断和治疗用途。

订购信息

产品名称产品编号规格价格数量收藏夹

beta Actin 抗体 (ACTBD11B7)

sc-81178
100 µg/ml
RMB2504.00

beta Actin (ACTBD11B7): m-IgG Fc BP-HRP 套装

sc-539599
100 µg Ab; 10 µg BP
RMB2662.00

beta Actin (ACTBD11B7): m-IgG1 BP-HRP 套装

sc-541587
100 µg Ab; 20 µg BP
RMB2662.00

Does this antibody recognize F-Actin (Filamentous) or G-Actin (Monomeric)?

Asked by: Alejandro
Thank you for your question. β-Actin (ACTBD11B7) antibody does not recognize F-Actin or G- Actin.
Answered by: BlakeJ
Date published: 2025-03-07

Does this product have lyophilized powder form,I got a bottle of this product which contains lyophilized powder. The Lot number is G1311. Thank you.

Asked by: wpihub
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2021-07-23

Bom dia,  Você poderia me enviar uma cotação formal em PDF deste produto? Atenciosamente, Amanda Souto Machado

Asked by: AmandaS
Thank you for your question. Please check the distributors details for your country on our web site: https://www.scbt.com/scbt/resources/distributors. They will be happy to provide for you the requested quotation.
Answered by: Technical Support Europe
Date published: 2017-06-14

Could I use sc-516132 m-IgG lambda -HRP as a secondary antibody with sc-81178 (b-Actin (ACTBD11B7)?

Asked by: Cary Stelloh
Thank you for question. β-Actin (ACTBD11B7): sc-81178, is a mouse monoclonal IgG1 with a kappa light chain. Therefore, you could not use the mouse IgG lambda light chain binding protein (sc-516132) as a secondary detection reagent with this primary. Instead, you would want to use our m-IgGκ BP-HRP: sc-516102 (https://www.scbt.com/scbt/product/m-igg-kappa-bp-hrp) which specifically recognizes the kappa light chain of mouse IgG.
Answered by: Technical Support
Date published: 2017-02-08

Hi, what is the specific type of this antibody? Is this kappa light chain? I need to decide which scondary antibody is to be used agianst this antibody.

Asked by: baizzang1
Thank you for your question. This is a kappa light chain mouse monoclonal antibody. While most anti-mouse IgG secondary antibodies are appropriate for use with this beta-Actin antibody, we recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety If you have any further questions, please contact our European Technical Service team. You can reach them by phone at Toll Free: +49 6221 4503 0, by email at: [email protected] or by live chat directly on our website, www.scbt.com
Answered by: Tech Service
Date published: 2017-01-17

What is the recommended secondary reagent for Western blot with this mouse monoclonal primary antibody?

Asked by: Anonymous
We recommend our Mouse IgG Binding Protein conjugated to HRP as a secondary detection reagent: https://www.scbt.com/scbt/product/m-igg-kappa-bp-hrp A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Tech Service 6
Date published: 2016-12-15

How can this mouse monoclonal antibody be used for double or triple staining, if the other primary antibodies are also raised in mouse?

Asked by: Anonymous
In this case, we recommend using a directly conjugated mouse monoclonal primary antibody. See β-Actin (C4): sc-47778 for β-Actin antibody conjugates, including HRP, FITC, PE, Alexa Fluor® 488 and Alexa Fluor® 647. https://www.scbt.com/scbt/product/beta-actin-antibody-c4
Answered by: Tech Service 6
Date published: 2016-12-15
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Rated 5 out of 5 by from Excellent!Excellent detection of b-actin in lymphoblastoid cell lines and K562 cells (last two lanes).
Date published: 2024-03-25
Rated 5 out of 5 by from The anti-β-actin antibody works wellFew months ago, we used the anti-β-actin antibody to determine the protein level in colon and liver tissues by Western blot. Both situation worked very well.
Date published: 2024-01-02
Rated 2 out of 5 by from not work wellThis antibodies does not work well. There are multiple bands.
Date published: 2021-01-02
Rated 5 out of 5 by from 功能很出色,结果很赞我之前买的试剂,结果非常棒也很特异,希望santa被更多人认可,被更多杂志接收,造福更多科研工作者,也希望未来有更多机会使用santa的产品
Date published: 2020-03-04
Rated 5 out of 5 by from Great antibodyI just got it few weeks ago, run my blot with it, and it looks great.
Date published: 2019-05-21
Rated 5 out of 5 by from Great antibodyThe antibody works great. It almost gives very specific bands.
Date published: 2019-01-18
Rated 5 out of 5 by from Product qualityI am working in cancer drug resistance. I took cancer drugs from Sigma but my experience was very bad that drugs were not even working. After that, I bought two drugs erlotinib and Gefitinib from Santa Cruz. Both are really working very nicely.
Date published: 2018-11-20
Rated 4 out of 5 by from Good qualityThe main band ans weak smaller one at moderate exposition: tested by Western blot and chemiluminesence detection.
Date published: 2018-06-23
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beta Actin 抗体 (ACTBD11B7) is rated 4.7 out of 5 by 28.
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