Date published: 2025-9-6

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beta Actin 抗体 (AC-15): sc-69879

4.8(40)
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说明书
  • beta Actin 抗体 AC-15 是小鼠单克隆 IgG1, 在1,303篇文献中引用,提供 100 µg/ml
  • 与β细胞质肌动蛋白的略微修饰的合成肽序列进行比对
  • 推荐用于 broad species 来源的β-Actin WB, IP 和 IF检测; 不建议用于检测adult cardiac and skeletal muscle actins or detection in Dictyostelium discoideum
  • 看 beta Actin (C4): sc-47778,可查看有标记的 beta Actin 抗体,包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • 关于如何获取beta Actin (AC-15): sc-69879免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前,我们还没有完成beta Actin 抗体 (AC-15)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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関連項目

β-Actin 抗体 (AC-15) 是一种小鼠单克隆 IgG1 抗体,它是针对与细胞质 β-Actin 相对应的略微修饰的合成肽而制备的,能够通过免疫印迹 (WB)、免疫沉淀 (IP) 和免疫荧光 (IF) 检测多种物种的 β-Actin。β-Actin 是一种高度保守的蛋白质,在维持细胞结构和完整性方面起着至关重要的作用,它是细胞骨架的主要成分,参与细胞运动、分裂和信号转导等各种细胞过程。在真核细胞中,β-Actin 通常占细胞蛋白质总量的 50%,并形成稳定和动态的丝状结构,对微绒毛的形成和肌肉收缩等功能至关重要。β-Actin 与多种蛋白质相互作用,包括促进肌肉收缩的肌球蛋白和肌动蛋白结合蛋白(如 profilin、cofilin 和 gelsolin),后者可调节肌动蛋白丝的聚合和解聚。β-肌动蛋白还与 Arp2/3 复合物结合,促进新肌动蛋白丝的成核,并与 Rho、Rac 和 Cdc42 等调节细胞骨架动态的小 GTP 酶相互作用;Rho 控制肌动蛋白应力纤维和局灶粘附的组装,Rac 促进肌动蛋白丝在质膜上的聚集,而 Cdc42 则刺激丝状体的形成。抗β-肌动蛋白抗体(AC-15)是研究人员在不同物种间研究肌动蛋白动力学、细胞结构和相关过程的宝贵工具。

仅限研究使用。不适用于诊断和治疗用途。

订购信息

产品名称产品编号规格价格数量收藏夹

beta Actin 抗体 (AC-15)

sc-69879
100 µg/ml
RMB2377.00

beta Actin (AC-15): m-IgG1 BP-HRP 套装

sc-551867
100 µg Ab; 20 µg BP
RMB2662.00

I didn't see (couldn't find yet) the list of species for which this reacts. Think it would work for molluscs? I need a loading control.....

Asked by: MoHU
Thank you for your question. We do not have a complete list of species reactivity for this β-Actin Antibody (AC-15): sc-69879. We know it is broadly reactive with mammalian and other non-mammalian species, however we are not aware specifically of any publication in mollusks.
Answered by: Technical Support
Date published: 2022-02-15

Can I use this mouse monoclonal antibody for immunohistochemical staining of mouse tissue?

Asked by: Cweed
Yes, we recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety Alternatively, SC-47778 is available directly conjugated to FITC, AF488, and AF647.
Answered by: TechService7
Date published: 2016-12-15

I need to detect beta-actin in bovine samples. Do you know if this mouse monoclonal antibody will work?

Asked by: Cweed
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: TechService7
Date published: 2023-09-08
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Rated 2 out of 5 by from No hubo reconocimiento de la proteínaAnalicé este anticuerpo para el reconocimiento de la actina en celulas C6/36 pero no hubo formacion de la banda esperada
Date published: 2024-12-09
Rated 5 out of 5 by from 綺麗なシングルバンドが出ます1ヶ月前に初めて試しましたが、5μgの蛋白量、1/1000希釈、室温1時間、2次抗体は1/1000で綺麗なシングルバンドが出ました。
Date published: 2024-09-27
Rated 5 out of 5 by from Useful for both WB and IFNot only can it be used for Western blotting, but we also tried immuno cyto fluorescent staining with MeOH fixation and PFA fixation. With PFA fixation, the staining pattern, which is typical of monoclonal antibodies against the cytoskeleton, showed a dotted staining only where the epitope was exposed, but with MeOH fixation showed a fibrous staining pattern.
Date published: 2024-08-17
Rated 5 out of 5 by from Excellent!Clear and strong signal in WB of mouse neural cells.
Date published: 2024-03-29
Rated 5 out of 5 by from Great for WBThe antibody is good for western blot. The band is single and clear.
Date published: 2024-03-20
Rated 5 out of 5 by from b-actin (sc-69879)It works well in western blot with 1:2000 dilution.
Date published: 2023-07-07
Rated 5 out of 5 by from SC-69879 - Anti -b Actin AC-15 in western blotWe are really satisfied of the Cod.SC-69879 - Anti -b Actin AC-15 Western Blot analysis in 10% SDS-PAGE of 40 μg of proteins from 4 brain tumour cell lines lysates. Anti-Actin Ab diluted 1:200; 4°C overnight.
Date published: 2022-08-11
Rated 5 out of 5 by from ExcellentI bought this antibody a few years ago and the result is good and clear.
Date published: 2021-11-08
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beta Actin 抗体 (AC-15) is rated 4.8 out of 5 by 40.
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