Date published: 2025-9-8
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Bestrophin Antibody (E6-6) - Western Blotting - Image 30901
Bestrophin 抗体 (E6-6): sc-32792. 非转染: sc-117752 (A) 和人 Bestrophin 转染: sc-113613 (B) 293T 全细胞裂解液中 Bestrophin 表达的 Western 印迹分析.
Bestrophin Antibody (E6-6) - Western Blotting - Image 30901
Bestrophin 抗体 (E6-6): sc-32792. 非转染: sc-117752 (A) 和人 Bestrophin 转染: sc-113613 (B) 293T 全细胞裂解液中 Bestrophin 表达的 Western 印迹分析.
Bestrophin Antibody (E6-6) - Western Blotting - Image 10094
Bestrophin 抗体 (E6-6): sc-32792. 猪视网膜上皮细胞组织提取物中 Bestrophin 表达的 Western 印迹分析.
Bestrophin 抗体 (E6-6): sc-32792. 非转染: sc-117752 (A) 和人 Bestrophin 转染: sc-113613 (B) 293T 全细胞裂解液中 Bestrophin 表达的 Western 印迹分析.

Bestrophin 抗体 (E6-6): sc-32792

3.7(3)
寫評論提問

说明书
  • Bestrophin 抗体 E6-6 是小鼠单克隆 IgG1 κ，Bestrophin抗体, 在4篇文献中引用，规格为200 µg/ml
  • 与human来源的C-terminus的bestrophin相对应的合成肽
  • Bestrophin 抗体 (E6-6) 推荐用于WB, IP, IF 和 IHC(P)，检测human 来源的 Bestrophin; 不会与 mouse or rat Bestrophin产生交联反应
  • 抗Bestrophin抗体（E6-6）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Bestrophin (E6-6): sc-32792的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG1 BP-HRP 是 Bestrophin 抗体 (E6-6) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Bestrophin 抗体 (E6-6) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 Bestrophin 抗体包括 Bestrophin-2

Bestrophin抗体（E6-6）是一种小鼠单克隆IgG1 kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和免疫组织化学检测人类来源的Bestrophin蛋白。抗Bestrophin抗体（E6-6）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。Bestrophin在维持视网膜健康方面发挥着至关重要的作用，它在视网膜色素上皮（RPE）中形成对细胞内钙离子水平敏感的低聚氯化物通道。这些通道对于调节视网膜中的离子传输和体液平衡至关重要，而视网膜对于感光细胞功能和整体视力至关重要。Bestrophin的突变可导致Best黄斑营养不良，即通常所说的Best病，其特征是脂褐素样物质在视网膜色素上皮细胞中积聚，导致视力逐渐丧失。人类Bestrophin编码一种含有585个氨基酸的蛋白质，该蛋白质包含四个推测的跨膜结构域，定位于RPE细胞的基底侧质膜。了解Bestrophin在离子转运中的功能和作用对于制定与其功能障碍相关的视网膜疾病的治疗策略至关重要。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Bestrophin 抗体 (E6-6) 参考文献:

  1. Bestrophin 是 Best 玻璃体黄斑营养不良症基因 (VMD2) 的产物，定位于视网膜色素上皮的基底侧质膜。  |  Marmorstein, AD., et al. 2000. Proc Natl Acad Sci U S A. 97: 12758-63. PMID: 11050159
  2. 黄斑变性的分子遗传学。  |  Musarella, MA. 2001. Doc Ophthalmol. 102: 165-77. PMID: 11556484
  3. Dbest1是果蝇与人类Bestrophin的同源物，但不需要Dbest1来维持生命力或感光细胞的完整性。  |  Tavsanli, BC., et al. 2001. Genesis. 31: 130-6. PMID: 11747204
  4. 玻璃体黄斑营养不良症蛋白定义了一个新的氯离子通道家族。  |  Sun, H., et al. 2002. Proc Natl Acad Sci U S A. 99: 4008-13. PMID: 11904445
  5. 导致贝斯特氏黄斑营养不良症（BMD）的基因编码一种假定的离子交换剂。  |  Gómez, A., et al. 2001. DNA Seq. 12: 431-5. PMID: 11913792
  6. Bestrophin 与蛋白磷酸酶 2A 存在物理和功能上的相互作用。  |  Marmorstein, LY., et al. 2002. J Biol Chem. 277: 30591-7. PMID: 12058047
  7. NEIBank 项目的人类 RPE/ 脉络膜表达序列标签分析：6000 多个非冗余转录本, 新基因和剪接变体。  |  Wistow, G., et al. 2002. Mol Vis. 8: 205-20. PMID: 12107410
  8. 黄斑萎缩  |  Braley, AE. 1966. Am J Ophthalmol. 61: 1-24. PMID: 5904376
  9. 鉴定导致贝斯特黄斑营养不良症的基因。  |  Petrukhin, K., et al. 1998. Nat Genet. 19: 241-7. PMID: 9662395

订购信息

产品名称产品编号规格价格数量收藏夹

Bestrophin 抗体 (E6-6)

sc-32792
200 µg/ml
RMB2377.00

Bestrophin (E6-6): m-IgG Fc BP-HRP 套装

sc-526689
200 µg Ab; 10 µg BP
RMB2662.00

Bestrophin (E6-6): m-IgG1 BP-HRP 套装

sc-532062
200 µg Ab; 20 µg BP
RMB2662.00

Bestrophin 抗体 (E6-6) AC

sc-32792 AC
500 µg/ml, 25% agarose
RMB3129.00

Bestrophin 抗体 (E6-6) HRP

sc-32792 HRP
200 µg/ml
RMB2377.00

Bestrophin 抗体 (E6-6) FITC

sc-32792 FITC
200 µg/ml
RMB2482.00

Bestrophin 抗体 (E6-6) PE

sc-32792 PE
200 µg/ml
RMB2580.00

Bestrophin 抗体 (E6-6) Alexa Fluor® 488

sc-32792 AF488
200 µg/ml
RMB2685.00

Bestrophin 抗体 (E6-6) Alexa Fluor® 546

sc-32792 AF546
200 µg/ml
RMB2685.00

Bestrophin 抗体 (E6-6) Alexa Fluor® 594

sc-32792 AF594
200 µg/ml
RMB2685.00

Bestrophin 抗体 (E6-6) Alexa Fluor® 647

sc-32792 AF647
200 µg/ml
RMB2685.00

Bestrophin 抗体 (E6-6) Alexa Fluor® 680

sc-32792 AF680
200 µg/ml
RMB2685.00

Bestrophin 抗体 (E6-6) Alexa Fluor® 790

sc-32792 AF790
200 µg/ml
RMB2685.00

Do I understand right: best1 antibody sc-32792 has been tested and does NOT detect rat or mouse best1? My application is fixed, frozen sections IF. If it hasn't been tested, could I test it? Otherwise can you recommend an alternative product for best1 ?

Asked by: best1 AB
Thank you for your question. It would be helpful if you could call 800-457-3801, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Service
Date published: 2024-01-25

Hi, My question is: Do you know what is the epitope sequence from sc-32792? ... Can you tell me?, because I can not see in information page about this antibody. Thanks

Asked by: Alejandro789
Thank you for your question. According to the literature, this monoclonal antibody, clone E6-6, was raised against peptide immunogen: KDHMDPYWALENRDEAHS. PMID: 11050159.
Answered by: Technical Support
Date published: 2019-08-16

What is the recommended starting dilution for Western blot for sc-32792: Bestrophin (E6-6) monoclonal antibody?

Asked by: cjMara
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-22
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Rated 5 out of 5 by from Good for immunofluorescenceThis antibody has been used to detect Bestrophin in RPE-differentiated human embryonic stem cells by immunofluorescence in published research. -SCBT QC
Date published: 2023-09-14
Rated 2 out of 5 by from Antibodies are not workingDear, I am sorry to say that this antibody did not work and gave me a lot of background. Of course using mice antibodies on mice tissue is not at all the best, but in my hand I had other antibodies working with less background. And moreover, the expected signal was not at all present, while the positive control worked very well. I tried 2 different dilutions 1:100 and 1: 500, but same results. I am sorry to say that, because usually Santa Cruz antibodies work very well...
Date published: 2018-03-29
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Bestrophin抗体 (E6-6) is rated 3.7 out of 5 by 3.
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