Date published: 2025-9-8

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BECN1/Beclin-1 抗体 (G-11): sc-48381

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说明书
  • BECN1/Beclin-1抗体(G-11)是小鼠单克隆IgG2b κ, 在70篇文献中引用,规格为200 µg/ml
  • 免疫human物种的BECN1的氨基酸1-300
  • 抗-BECN1/Beclin-1 抗体 (G-11) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 Beclin 1
  • 抗BECN1/Beclin-1抗体(G-11)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取BECN1/Beclin-1 (G-11): sc-48381免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRPm-IgG2b BP-HRP 是 BECN1/Beclin-1 抗体 (G-11) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与BECN1/Beclin-1 抗体 (G-11) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

Beclin 1 抗体 (G-11) 是一种小鼠单克隆 IgG2b kappa 轻链抗体,设计用于检测小鼠、大鼠和人类的 Beclin 1,使抗 Beclin 1 抗体 (G-11) 成为 Western 印迹 (WB) 中不可或缺的试剂、 免疫沉淀(IP)、免疫荧光(IF)、石蜡包埋切片免疫组织化学(IHCP)和酶联免疫吸附试验(ELISA)中不可或缺的试剂。Beclin 1 单克隆抗体 (G-11) 是针对人 Beclin 1 的 1-300 个氨基酸制备的,既有非共轭形式,也有多种共轭形式,如琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和各种 Alexa Fluor® 共轭物,可满足不同的实验要求。Beclin 1 又称 BECN1、ATG6、VPS30 和 beclin1,是一种重要的盘绕线圈蛋白,主要定位于细胞质中,在细胞质中 Beclin 1 与内质网、线粒体和高尔基体的膜结合,协调自噬途径中的一个基本步骤--自噬体的形成。自噬对细胞平衡至关重要,它能降解和再循环受损的蛋白质和细胞器,并在防止细胞失控生长和肿瘤发生方面发挥重要作用。通过与 Bcl-2 相互作用,Beclin 1 可调节自噬活性,从而维持细胞的完整性并应对压力条件。Beclin 1 的细胞定位协调自噬体形成的空间和时间方面,这对有效的自噬通量和细胞存活至关重要。在 MCF7 等多种癌细胞系中观察到,与正常组织中的普遍表达相比,Beclin 1 的表达量有所降低,这突出表明 Beclin 1 在抑制癌症发展方面的作用。此外,Beclin 1与酵母自噬基因产物Apg6在结构上有相似之处,这表明自噬功能在不同生物体中是一致的。在转染细胞中,Beclin 1 不仅能补充自噬,还能抑制细胞增殖,进一步强调了其在调节细胞生长和维持细胞平衡方面的重要作用。总之,Beclin 1 (G-11) 抗体可提供可靠、多功能的 Beclin 1 检测,是研究自噬分子机制、癌症生物学和相关细胞过程的研究人员必不可少的工具。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

BECN1/Beclin-1 抗体 (G-11)

sc-48381
200 µg/ml
RMB2377.00

BECN1/Beclin-1 (G-11): m-IgG Fc BP-HRP 套装

sc-525561
200 µg Ab; 10 µg BP
RMB2662.00

BECN1/Beclin-1 (G-11): m-IgG2b BP-HRP 套装

sc-549583
200 µg Ab; 10 µg BP
RMB2662.00

BECN1/Beclin-1 抗体 (G-11) AC

sc-48381 AC
500 µg/ml, 25% agarose
RMB3129.00

BECN1/Beclin-1 抗体 (G-11) HRP

sc-48381 HRP
200 µg/ml
RMB2377.00

BECN1/Beclin-1 抗体 (G-11) FITC

sc-48381 FITC
200 µg/ml
RMB2482.00

BECN1/Beclin-1 抗体 (G-11) PE

sc-48381 PE
200 µg/ml
RMB2580.00

BECN1/Beclin-1 抗体 (G-11) Alexa Fluor® 488

sc-48381 AF488
200 µg/ml
RMB2685.00

BECN1/Beclin-1 抗体 (G-11) Alexa Fluor® 546

sc-48381 AF546
200 µg/ml
RMB2685.00

BECN1/Beclin-1 抗体 (G-11) Alexa Fluor® 594

sc-48381 AF594
200 µg/ml
RMB2685.00

BECN1/Beclin-1 抗体 (G-11) Alexa Fluor® 647

sc-48381 AF647
200 µg/ml
RMB2685.00

BECN1/Beclin-1 抗体 (G-11) Alexa Fluor® 680

sc-48381 AF680
200 µg/ml
RMB2685.00

BECN1/Beclin-1 抗体 (G-11) Alexa Fluor® 790

sc-48381 AF790
200 µg/ml
RMB2685.00

The anti-becn1 whether immunofluorescence staining of zebrafish larvae was supported?

Asked by: xiaom
Thank you for your question. Anti-BECN1/Beclin-1 Antibody (G-11) is recommended for detection of Beclin 1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA. It would be helpful if you could email us directly at [email protected]. So we can have a more interactive discussion about this and other related issues.
Answered by: Zhanglong
Date published: 2023-05-12

What is the difference between BECN1 Antibody (E-8): sc-48341 and (G-11): sc-48381?

Asked by: luke50
Thank you for your questions. These are two different monoclonal antibodies against BECN1 that were raised against the same antigen. They represent distinct epitopes, and have different isotypes, however their performance and reactivity is very similar.
Answered by: Technical Support
Date published: 2017-10-24

Can you recommend a positive control to use with BECN1 (G-11): sc-48381 monoclonal antibody for Western blot?

Asked by: jenniferc
We recommend MCF7: sc-2206, HeLa: sc-2200, A-431: sc-2201, Raji: sc-364236 or Jurkat: sc-2204 whole cell lysates as we have great Western blot data showing specific detection of BECN1 in these cell lines.
Answered by: Technical Support 15
Date published: 2017-01-10
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Rated 3 out of 5 by from Average for WBWork OK for western blot in rat renal tubular cells.
Date published: 2023-08-18
Rated 4 out of 5 by from It is suitable for IHCIt is suitable for IHC staining, therefore, I suggest it
Date published: 2022-04-07
Rated 3 out of 5 by from Bastante buena señal.Se detectan dos bandas. MUetsras de celulas estrelladas.
Date published: 2020-05-09
Rated 4 out of 5 by from Nice antibodyNice antibody when you are working with pp70s6. It works well for WB with dilution 1:200, specific band
Date published: 2017-10-26
Rated 4 out of 5 by from GoodI had tested it in C42B cell lines. It works good but have a lot of non-specific band in other place.
Date published: 2017-10-11
Rated 5 out of 5 by from very goodThe expression of beclin1 changed significantly in our experiment. This antibody helps us so much!
Date published: 2017-09-07
Rated 5 out of 5 by from Excellent antibody for IHC applicationsThe BECN1 Antibody (G-11): sc-48381 works great for immunohistochemistry with paraffin-embedded sections, showing strong staining pattern localized to the cytoplasm.
Date published: 2016-12-20
Rated 5 out of 5 by from This has worked well in WBThis has worked well in WB.
Date published: 2015-11-18
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BECN1/Beclin-1 抗体 (G-11) is rated 4.5 out of 5 by 15.
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