Date published: 2025-9-8
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band 3 Antibody (A-6) - Western Blotting - Image 355799
band 3 抗体 (A-6): sc-133190. K-562 (A), CCRF-CEM (B), MEG-01 (C), MOLT-4 (D) 和 HL-60 (E) 全细胞裂解液中 band 3 表达的 Western 印迹分析.
band 3 抗体 (A-6): sc-133190. K-562 (A), CCRF-CEM (B), MEG-01 (C), MOLT-4 (D) 和 HL-60 (E) 全细胞裂解液中 band 3 表达的 Western 印迹分析.

band 3 抗体 (A-6): sc-133190

5.0(6)
寫評論提問

说明书
  • band 3 抗体 A-6 是小鼠单克隆 IgG2a κ，band 3抗体, 在9篇文献中引用，规格为200 µg/ml
  • 针对氨基酸1-105，在band 3的human起源的N-terminus处进行映射
  • band 3 抗体 (A-6) 推荐用于WB, IP, IF, IHC(P) 和 ELISA，检测human 来源的 band 3
  • 抗band 3抗体（A-6）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取band 3 (A-6): sc-133190的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG2a BP-HRP 和 m-IgGκ BP-HRP 是 band 3 抗体 (A-6) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与band 3 抗体 (A-6) 组合成套装提供。 (请参阅下面的订购信息)。

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相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

Band 3抗体（A-6）是一种IgG2a κ小鼠单克隆Band 3抗体（也称为Band 3抗体），可通过WB、IP、IF、IHC（P）和ELISA检测人源的Band 3蛋白。Band 3抗体（A-6）既可以是非偶联的抗Band 3抗体形式，也可以是多种偶联形式的抗Band 3抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。Band 3，也称为AE1，是一种红细胞膜糖蛋白，有助于细胞结构完整性并介导氯离子和碳酸氢根离子跨磷脂双层的交换。这个约由900个氨基酸组成的蛋白质，其多样化的功能由两个不同的结构域所介导。氨基末端结构域，也称为红细胞膜带3的细胞质结构域cdb3，通过结合锚蛋白作为红细胞骨架的附着位点。羧基末端、膜相关结构域则执行阴离子的交换转运。Band 3的降解可以产生一种被称为衰老细胞抗原（SCA）的衰老抗原，这种抗原在衰老的细胞上表达，并标记它们以供免疫系统清除。在肾脏中表达的一种Band 3同工型缺少前65个氨基酸，并且不结合锚蛋白。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

band 3 抗体 (A-6) 参考文献:

  1. Caspase 3 介导的人红细胞阴离子交换子 1（band 3）N 端细胞质结构域蛋白水解。  |  Mandal, D., et al. 2003. J Biol Chem. 278: 52551-8. PMID: 14570914
  2. 人红细胞 3 号带蛋白 C 端结构域的纯化和特征描述。  |  Fu, G., et al. 2004. Biochemistry. 43: 1633-8. PMID: 14769040
  3. 人类红细胞阴离子转运蛋白。部分氨基酸序列, 构象和阴离子交换的可能分子机制。  |  Brock, CJ., et al. 1983. Biochem J. 213: 577-86. PMID: 6615451
  4. 人类肾脏和肿瘤细胞瘤中的阴离子交换子 1 在其 NH2 末端与红细胞 AE1 不同。  |  Kollert-Jöns, A., et al. 1993. Am J Physiol. 265: F813-21. PMID: 7506871
  5. 带 3 在平衡和细胞形状中的作用  |  Jay, DG. 1996. Cell. 86: 853-4. PMID: 8808620
  6. 带 3 蛋白与体积激活的阴离子和氨基酸通道的关联：一种分子方法。  |  Motais, R., et al. 1997. J Exp Biol. 200: 361-7. PMID: 9050245
  7. 带 3（AE1）的结构和功能：最新进展（综述）。  |  Tanner, MJ. 1997. Mol Membr Biol. 14: 155-65. PMID: 9491367

订购信息

产品名称产品编号规格价格数量收藏夹

band 3 抗体 (A-6)

sc-133190
200 µg/ml
RMB2377.00

band 3 (A-6): m-IgG Fc BP-HRP 套装

sc-528763
200 µg Ab; 10 µg BP
RMB2662.00

band 3 (A-6): m-IgGκ BP-HRP 套装

sc-521268
200 µg Ab, 40 µg BP
RMB2662.00

band 3 (A-6): m-IgG2a BP-HRP 套装

sc-547161
200 µg Ab; 10 µg BP
RMB2662.00

band 3 抗体 (A-6) AC

sc-133190 AC
500 µg/ml, 25% agarose
RMB3129.00

band 3 抗体 (A-6) HRP

sc-133190 HRP
200 µg/ml
RMB2377.00

band 3 抗体 (A-6) FITC

sc-133190 FITC
200 µg/ml
RMB2482.00

band 3 抗体 (A-6) PE

sc-133190 PE
200 µg/ml
RMB2580.00

band 3 抗体 (A-6) Alexa Fluor® 488

sc-133190 AF488
200 µg/ml
RMB2685.00

band 3 抗体 (A-6) Alexa Fluor® 546

sc-133190 AF546
200 µg/ml
RMB2685.00

band 3 抗体 (A-6) Alexa Fluor® 594

sc-133190 AF594
200 µg/ml
RMB2685.00

band 3 抗体 (A-6) Alexa Fluor® 647

sc-133190 AF647
200 µg/ml
RMB2685.00

band 3 抗体 (A-6) Alexa Fluor® 680

sc-133190 AF680
200 µg/ml
RMB2685.00

band 3 抗体 (A-6) Alexa Fluor® 790

sc-133190 AF790
200 µg/ml
RMB2685.00

Is this antibody (sc-133190) affinity purified?

Asked by: Stephen1977
Thank you for your question. This mouse monoclonal antibody is not affinity purified against the antigen. -Tech Service
Answered by: Tech Service 1
Date published: 2021-01-28

Hello， Does this bind3 antibody work for Facs？

Asked by: Paul_lee
Thank you for your question. This antibody has not yet been tested for FACS. The conjugated primary antibody is recommended and warrantied for flow cytometry (FCM), however we are still gathering data to show on our website. Check back for updates!
Answered by: Technical Support
Date published: 2019-07-13

I am using band 3 (A-6): sc-133190 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: AbPolly
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Super!The product has been tested in western and dot blot against samples derived from human red blood cells (extracellular vesicles and cellular extract, 30ug per lane). Tests were run at 1:500 and 1:1000 dilution in PBS + 0.05% tween-20 + 1% fat-free milk (WB analysis) and PBS + 0.005% tween-20 + 1% fat-free milk (Dot blot analysis). The protein has been detected around 95 kDa, as reported in the data sheet. We could detect a very strong signal in both samples. We truly recommend this antibody for the detection of red blood cell-derived samples.
Date published: 2023-03-23
Rated 5 out of 5 by from Excellent!This antibody works great in western blot analysis of band 3 expression in K-562, CCRF-CEM, MEG-01, MOLT-4 and HL-60 whole cell lysates.
Date published: 2017-02-06
Rated 5 out of 5 by from Used this antibody in WB in human cells andUsed this antibody in WB in human cells and works great, happy with it.
Date published: 2015-08-24
Rated 5 out of 5 by from Produced a clear band with HeLa whole cell lysateProduced a clear band with HeLa whole cell lysate. -SCBT QC
Date published: 2015-06-17
Rated 5 out of 5 by from Produced a clear band with HEL 92Produced a clear band with HEL 92.1.7, K-562 and Hep G2 whole cell lysates and human liver tissue extract. -SCBT QC
Date published: 2015-04-23
Rated 5 out of 5 by from Produced beautiful immunoperoxidase membraneProduced beautiful immunoperoxidase membrane staining in formalin fixed, paraffin-embedded human blood vessel tissue. -SCBT QC
Date published: 2013-02-01
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band 3 抗体 (A-6) is rated 5.0 out of 5 by 6.
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