Date published: 2025-9-7

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BAG-6 抗体 (D-1): sc-365928

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说明书
  • BAG-6 抗体 D-1 是小鼠单克隆 IgG1 κ,BAG-6抗体, 在14篇文献中引用,规格为200 µg/ml
  • 免疫原氨基酸序列833-982位于human物种的BAG-6的C-terminus
  • BAG-6 抗体 (D-1) 推荐用于 WB, IP, IF 和 ELISA,检测mouse, rat 和human 来源的 BAG-6
  • 抗BAG-6抗体(D-1)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取BAG-6 (D-1): sc-365928免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgGκ BP-HRP是BAG-6 Antibody (D-1) 适用于 WB 应用。 的首选辅助检测试剂。该试剂现可与BAG-6 Antibody (D-1) 搭配使用(请参阅下面的订购信息)。有关其它 m-IgGκ BP 结合物,请参阅我们完整的小鼠 IgG 结合蛋白列表。

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関連項目

BAG-6 Antibody (D-1)是一种IgG1 κ的小鼠单克隆BAG-6抗体(也称为BAG-6抗体),通过WB、IP、IF和ELISA检测小鼠、大鼠和人源的BAG-6蛋白。BAG-6抗体(D-1)既可以是非偶联的抗BAG-6抗体形式,也可以是多种偶联的抗BAG-6抗体形式,包括琼脂糖,HRP, PE, FITC和多个Alexa Fluor®偶联物。BAT3 (HLA-B相关转录本3)又称G3、scythe、BAG6或D6S52E,是一种富含脯氨酸的核蛋白,具有重要的凋亡调节作用。BAT3的n端含有1个泛素样结构域,c端含有2个核定位信号。具体来说,BAT3与凋亡诱导因子(apoptosis inducing factor, AIF)相互作用并稳定,从而使细胞对内质网应激介导的凋亡敏感。在蓖麻毒素处理后,BAT3被半胱氨酸天冬氨酸蛋白酶-3 (caspase-3)切割,其c末端片段显示出促凋亡活性。凋亡活动包括细胞核凝聚、磷脂酰丝氨酸外化、细胞圆缩。BAT3缺陷的小鼠在肺、脑和肾发育以及增殖和凋亡的调节方面表现出明显的缺陷。这些缺陷最终导致围生期或妊娠中期死亡。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

BAG-6 抗体 (D-1) 参考文献:

  1. 大鼠 BAT3 cDNA 的克隆和特征描述。  |  Ozaki, T., et al. 1999. DNA Cell Biol. 18: 503-12. PMID: 10390159
  2. 人类 Scythe 含有功能性核定域序列,并在士的宁诱导细胞凋亡过程中保持在细胞核中。  |  Manchen, ST. and Hubberstey, AV. 2001. Biochem Biophys Res Commun. 287: 1075-82. PMID: 11587531
  3. 分析高通量酵母双杂交系统及其在预测人类 MHC III 类区域内编码的细胞内蛋白功能中的应用。  |  Lehner, B., et al. 2004. Genomics. 83: 153-67. PMID: 14667819
  4. 蓖麻毒素通过Caspase-3裂解BAT3引发凋亡形态变化。  |  Wu, YH., et al. 2004. J Biol Chem. 279: 19264-75. PMID: 14960581
  5. 蛛网膜下腔出血后,内皮细胞NOX4通过降低二氢叶酸还原酶来加剧eNOS解偶联。  |  Winnefeld, M., et al. 2006. Exp Cell Res. 312: 2500-14. PMID: 16777091
  6. 基于表型鉴定非洲猪瘟病毒复制所需的宿主基因。  |  Chang, AC., et al. 2006. J Virol. 80: 8705-17. PMID: 16912318
  7. HLA-B 相关转录本 3 (Bat3)/Scythe 对于 p300 介导的 p53 乙酰化至关重要。  |  Sasaki, T., et al. 2007. Genes Dev. 21: 848-61. PMID: 17403783
  8. 人类白细胞抗原-B-缔合作用转录本 3 从肿瘤细胞中释放出来,并与自然杀伤细胞上的 NKp30 受体接合。  |  Pogge von Strandmann, E., et al. 2007. Immunity. 27: 965-74. PMID: 18055229
  9. Scythe 在内质网应激诱导细胞凋亡过程中调节凋亡诱导因子的稳定性。  |  Desmots, F., et al. 2008. J Biol Chem. 283: 3264-3271. PMID: 18056262
  10. BAG-6对于选择性消除缺陷蛋白酶体底物至关重要。  |  Minami, R., et al. 2010. J Cell Biol. 190: 637-50. PMID: 20713601
  11. 肿瘤抗原BCL2-相关athanogene 6 (BAG-6)的可溶性片段对于抑制自然杀伤细胞的NKp30受体依赖性细胞毒性是必要且足够的。  |  Binici, J., et al. 2013. J Biol Chem. 288: 34295-303. PMID: 24133212
  12. 人类主要组织相容性复合体中的一个新基因簇。  |  Spies, T., et al. 1989. Science. 243: 214-7. PMID: 2911734

订购信息

产品名称产品编号规格价格数量收藏夹

BAG-6 抗体 (D-1)

sc-365928
200 µg/ml
RMB2377.00

BAG-6 (D-1): m-IgGκ BP-HRP 套装

sc-522427
200 µg Ab, 40 µg BP
RMB2662.00

BAG-6 抗体 (D-1) AC

sc-365928 AC
500 µg/ml, 25% agarose
RMB3129.00

BAG-6 抗体 (D-1) HRP

sc-365928 HRP
200 µg/ml
RMB2377.00

BAG-6 抗体 (D-1) FITC

sc-365928 FITC
200 µg/ml
RMB2482.00

BAG-6 抗体 (D-1) PE

sc-365928 PE
200 µg/ml
RMB2580.00

BAG-6 抗体 (D-1) Alexa Fluor® 488

sc-365928 AF488
200 µg/ml
RMB2685.00

BAG-6 抗体 (D-1) Alexa Fluor® 546

sc-365928 AF546
200 µg/ml
RMB2685.00

BAG-6 抗体 (D-1) Alexa Fluor® 594

sc-365928 AF594
200 µg/ml
RMB2685.00

BAG-6 抗体 (D-1) Alexa Fluor® 647

sc-365928 AF647
200 µg/ml
RMB2685.00

BAG-6 抗体 (D-1) Alexa Fluor® 680

sc-365928 AF680
200 µg/ml
RMB2685.00

BAG-6 抗体 (D-1) Alexa Fluor® 790

sc-365928 AF790
200 µg/ml
RMB2685.00

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: jerojero
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Tech Service 3
Date published: 2017-02-01
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Rated 5 out of 5 by from the good antibodythis antibody is suitable for detecting BAG6 protein in murine and pig testis. we have successfully use it for WB and Co-IP assays.
Date published: 2023-03-04
Rated 5 out of 5 by from Great antibodyGot a trial sample from Santa Cruz and it works well. I used it for detection of Bag-6 in HeLa cell total extract at a working dilution of 1:100.
Date published: 2019-11-17
Rated 5 out of 5 by from Great AntibodyGot a trial sample from Santa Cruz. The antibody is great with nice clean band in SH-SY5Y cells. Used at 1:500 dilution
Date published: 2018-06-20
Rated 5 out of 5 by from Strong Intensity in IFThis antibody gave us a high intensity IF signal for HeLa cells
Date published: 2016-12-20
Rated 5 out of 5 by from Robust signal in WB in NTERARobust signal in WB in NTERA-2 cl.D1 and U-87 MG whole cell lysates. -SCBT QC
Date published: 2015-07-20
Rated 5 out of 5 by from Good specificity in WB in A431Good specificity in WB in A431, MCF7, HEK293 and HepG2 whole cell lysates. -SCBT QC
Date published: 2015-02-15
Rated 5 out of 5 by from WB and IF publishable data with mouse HELAWB and IF publishable data with mouse HELA cell and N2a cell - SCBT Publication Review
Date published: 2015-01-22
Rated 5 out of 5 by from Great immunofluorescence nuclear and cytoplasmicGreat immunofluorescence nuclear and cytoplasmic staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2014-01-28
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BAG-6 抗体 (D-1) is rated 5.0 out of 5 by 8.
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