Date published: 2025-9-6
访问圣克鲁斯动物健康

021-6093-6350

SCBT Portrait Logo
Seach Input
联系我们
登陆账户
快速订购
购物车 (0)
BACH1 Antibody (F-9) - Western Blotting - Image 390739
BACH1 抗体 (F-9) Alexa Fluor® 647: sc-271211 AF647. 对 Raji (A), HEL 92. 1. 7 (B) 和 NTERA-2 cl. D1 (C) 全细胞裂解液中 BACH1 表达的直接荧光西部印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断.
BACH1 抗体 (F-9) Alexa Fluor® 647: sc-271211 AF647. 对 Raji (A), HEL 92. 1. 7 (B) 和 NTERA-2 cl. D1 (C) 全细胞裂解液中 BACH1 表达的直接荧光西部印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断.

BACH1 抗体 (F-9): sc-271211

4.8(12)
寫評論提問

说明书
  • BACH1 抗体 F-9 是小鼠单克隆 IgG1 κ，BACH1抗体, 在53篇文献中引用，规格为200 µg/ml
  • 免疫原氨基酸序列591-720位于human物种的BACH1的C-terminus附近
  • BACH1 抗体 (F-9) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 BACH1
  • 抗BACH1抗体（F-9）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于ChIP的TransCruz试剂(sc-271211 X, 200 µg/0.1 ml)
  • 关于如何获取BACH1 (F-9): sc-271211的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP、 1 BP-HRP">m-IgG1 BP-HRP和m-IgGκ BP-HRP是BACH1 Antibody (F-9) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与BACH1 Antibody (F-9) 打包提供（请参阅下面的订购信息）。

快捷链接

相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

BACH抗体

BACH1抗体（F-9）是一种IgG1 κ小鼠单克隆BACH1抗体（也称为BTB结构域和CNC同源物1抗体、BTB和CNC同源物1碱性亮氨酸拉链转录因子1抗体、转录调节蛋白BACH1抗体、BACH-1抗体、BTBD24抗体、碱性区域亮氨酸拉链转录调节因子BACH1抗体或BTB和CNC同源物1抗体），可通过WB、IP、IF和ELISA检测到来自小鼠、大鼠和人类的BACH1蛋白。BACH1抗体（F-9）既有非偶联形式的抗BACH1抗体，也有多种偶联形式的抗BACH1抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。小型Maf家族成员（MafK、MafF和MafG）是碱性区域亮氨酸拉链（bZip）蛋白，可作为转录激活剂或抑制剂。它们与其他蛋白质二聚化，并根据二聚体组成结合DNA以抑制或激活转录。BACH1和BACH2是MafK的异二聚化伴侣，属于新型BTB/POZ-碱性区域亮氨酸拉链（bzip）因子家族成员。BACH1和BACH2在BTB结构域和Cap 'n' collar型bZip结构域中具有显著的相似性，但在其他方面则不同。BACH1似乎无处不在，而BACH2则仅限于单核细胞和神经元细胞，并在B细胞分化的早期阶段大量表达。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

BACH1 抗体 (F-9) 参考文献:

  1. 转录因子 Bach2 和 MAZR（MAZ 相关因子）通过 BTB/POZ 结构域产生的基因表达组合代码。  |  Kobayashi, A., et al. 2000. Mol Cell Biol. 20: 1733-46. PMID: 10669750
  2. 氧化应激取消了对左旋霉素 B 敏感的转录抑制因子 Bach2 的核输出，从而抵消了 Maf 识别元件的激活。  |  Hoshino, H., et al. 2000. J Biol Chem. 275: 15370-6. PMID: 10809773
  3. 人类 B 细胞特异性转录因子 BACH2 在染色体 6q15 上的克隆和表达。  |  Sasaki, S., et al. 2000. Oncogene. 19: 3739-49. PMID: 10949928
  4. 转录因子 BACH1 通过其新型替代剪接异构体被招募到细胞核中。  |  Kanezaki, R., et al. 2001. J Biol Chem. 276: 7278-84. PMID: 11069897
  5. 针对 BACH1 和线粒体代谢的有效乳腺癌联合疗法。  |  Lee, J., et al. 2019. Nature. 568: 254-258. PMID: 30842661
  6. 铁突变：通过 NRF2 和 BACH1 之间的竞争以及死亡信号的传播进行调节。  |  Nishizawa, H., et al. 2023. FEBS J. 290: 1688-1704. PMID: 35107212
  7. BACH1诱导的铁变态反应通过抑制单不饱和脂肪酸的生物合成驱动淋巴转移。  |  Xie, X., et al. 2023. Cell Death Dis. 14: 48. PMID: 36670112
  8. FBXO22 通过靶向 MLL 重组急性髓性白血病中的 BACH1 促进白血病的发生。  |  Zhu, XN., et al. 2023. J Hematol Oncol. 16: 9. PMID: 36774506
  9. BACH1 缺乏症可通过调节染色质的可及性防止新血管内膜的形成并维持血管平滑肌细胞的分化表型。  |  Guo, J., et al. 2023. Nucleic Acids Res. 51: 4284-4301. PMID: 36864760
  10. Lnc AC016727.1/BACH1/HIF-1 α 信号环促进非小细胞肺癌的进展。  |  Zhang, L., et al. 2023. J Exp Clin Cancer Res. 42: 296. PMID: 37946265
  11. 癌症中的转录因子 BACH1：作用、机制和靶向治疗前景。  |  Hu, D., et al. 2024. Biomark Res. 12: 21. PMID: 38321558
  12. Bach 蛋白属于 BTB-基本亮氨酸拉链转录因子的一个新家族，它们与 MafK 相互作用，通过 NF-E2 位点调节转录。  |  Oyake, T., et al. 1996. Mol Cell Biol. 16: 6083-95. PMID: 8887638

订购信息

产品名称产品编号规格价格数量收藏夹

BACH1 抗体 (F-9)

sc-271211
200 µg/ml
RMB2377.00

BACH1 (F-9): m-IgG Fc BP-HRP 套装

sc-529141
200 µg Ab; 10 µg BP
RMB2662.00

BACH1 (F-9): m-IgGκ BP-HRP 套装

sc-521879
200 µg Ab, 40 µg BP
RMB2662.00

BACH1 (F-9): m-IgG1 BP-HRP 套装

sc-543347
200 µg Ab; 20 µg BP
RMB2662.00

BACH1 抗体 (F-9) X

sc-271211 X
200 µg/0.1 ml
RMB2377.00

BACH1 抗体 (F-9) AC

sc-271211 AC
500 µg/ml, 25% agarose
RMB3129.00

BACH1 抗体 (F-9) HRP

sc-271211 HRP
200 µg/ml
RMB2377.00

BACH1 抗体 (F-9) FITC

sc-271211 FITC
200 µg/ml
RMB2482.00

BACH1 抗体 (F-9) PE

sc-271211 PE
200 µg/ml
RMB2580.00

BACH1 抗体 (F-9) Alexa Fluor® 488

sc-271211 AF488
200 µg/ml
RMB2685.00

BACH1 抗体 (F-9) Alexa Fluor® 546

sc-271211 AF546
200 µg/ml
RMB2685.00

BACH1 抗体 (F-9) Alexa Fluor® 594

sc-271211 AF594
200 µg/ml
RMB2685.00

BACH1 抗体 (F-9) Alexa Fluor® 647

sc-271211 AF647
200 µg/ml
RMB2685.00

BACH1 抗体 (F-9) Alexa Fluor® 680

sc-271211 AF680
200 µg/ml
RMB2685.00

BACH1 抗体 (F-9) Alexa Fluor® 790

sc-271211 AF790
200 µg/ml
RMB2685.00

I am using sc-271211 in A7r5 cells. I extracted nuclear and cytoplasmic protein and run western blot using 1:100 ratio in 5% milk. However, I am not able to see the band, even though I can detect inner control band and did membrane transfer properly.

Asked by: ypharan
Thank you for your product review. We are sorry to hear that this antibody did not perform for you as expected. We want you to be successful in your research. Please contact our Technical Service Department by phone, e-mail or live chat. We are happy to help you troubleshoot the antibody.
Answered by: Tech Service
Date published: 2019-04-09

I am interested in trying out the BACH1 (F-9) antibody. How can I get a FREE 10 µg sample of BACH1 (F-9): sc-271211?

Asked by: Harinath
Please call us Toll Free at 800-457-3801 for free mono sample, or email us via [email protected], or by live chat on our website.
Answered by: Technical Support 2
Date published: 2017-09-21

Can the TransCruz reagent sc-271211 X be used for applications other than ChIP?

Asked by: AbPolly
Thank you for your question. Yes, the X form of the antibody can be used for other listed applications such as WB and IF, the dilution factor just needs to be adjusted as the X form is ten times as concentrated as the standard antibody.
Answered by: Technical Service
Date published: 2017-02-02
  • y_2025, m_8, d_30, h_9CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_3
  • loc_zh_CN, sid_271211, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 112ms
  • QUESTIONS, PRODUCT
Rated 3 out of 5 by from OK for WBNot the best antibody (signal is low), but gets the job done. Have used it for WB on 293T lysates.
Date published: 2024-04-08
Rated 5 out of 5 by from Works as intended, good sensitivityWorks as intended, used to probe BACH1 in WBs (1:1000) in cancer cell lines and in HEK293T exogenous expression.
Date published: 2023-12-21
Rated 5 out of 5 by from Ottimo funzionamento, 1h a RTHo testato questo anticorpo su lisato di cellule endoteliali umane e ha mostrato una banda specifica e chiara.
Date published: 2022-04-07
Rated 5 out of 5 by from works great!I got the free sample of BACH1 F-9 antibody, it works great at a dilution of 1:500.
Date published: 2020-09-08
Rated 5 out of 5 by from reliable antibody for WBspecific and clear signal in WB, when diluted 1:500 in BSA/TBS-T. Works well for HEp-2, RKO and LoVo whole cell lysates.
Date published: 2020-01-09
Rated 5 out of 5 by from wbit's have a specific bands above 90 kd we use 1:200 diulite in wb.
Date published: 2019-04-09
Rated 5 out of 5 by from The sample Antibody worked with WBI used 1:200 of this antibody to stain blots and detected it at 130 KDA
Date published: 2018-10-29
Rated 5 out of 5 by from WBWe followed the WB protocol and obtained,very little background and strong target band.We often use this antibody detect human breast tumor tissue,very satisfied result.
Date published: 2018-01-27
  • y_2025, m_8, d_30, h_9
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_12
  • loc_zh_CN, sid_271211, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 18ms
  • REVIEWS, PRODUCT
BACH1 抗体 (F-9) is rated 4.8 out of 5 by 12.
  • y_2025, m_8, d_30, h_9
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_12
  • loc_zh_CN, sid_271211, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 98ms
  • REVIEWS, PRODUCT
021-6093-6350
访问 Santa Cruz Animal Health|访问 San Juan Ranch
Santa Cruz Biotechnology, Inc. 是生物医学研究市场产品开发的世界领导者。请拨打0086-021-60936350联系我们。
版权所有© 2007-2025 Santa Cruz Biotechnology, Inc. 保留所有权利。”Santa Cruz Biotechnology” 和Santa Cruz Biotechnology, Inc.的商标，”Santa Cruz Animal Health”, “San Juan Ranch”, “Supplement of Champions”, San Juan Ranch的商标，”UltraCruz”, “ChemCruz”, “ImmunoCruz”, “ExactaCruz”, 和 “EZ Touch”是Santa Cruz Biotechnology, Inc.的注册商标。所有商标均为其各自所有者的财产。