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B42 Antibody (E-2) - Western Blotting - Image 281630
B42 抗体 (E-2): sc-377101. 全长 B42 融合蛋白 (sc-4269) 的 Western 印迹分析.
B42 Antibody (E-2) - Western Blotting - Image 281630
B42 抗体 (E-2): sc-377101. 全长 B42 融合蛋白 (sc-4269) 的 Western 印迹分析.
B42 抗体 (E-2): sc-377101. 全长 B42 融合蛋白 (sc-4269) 的 Western 印迹分析.

B42 抗体 (E-2): sc-377101

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说明书
  • B42 抗体 E-2 是小鼠单克隆 IgG1 (kappa轻链)，B42抗体，规格为200 µg/ml
  • 特异性针对B42转录激活域内部区域氨基酸57-93之间的表位图谱
  • B42 抗体 (E-2) 推荐用于 WB, IP, IF 和 ELISA，检测 B42 fusion proteins
  • 抗B42抗体（E-2）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取B42 (E-2): sc-377101的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP和 1 BP-HRP">m-IgG1 BP-HRP是B42 Antibody (E-2) for WB applications. 的首选辅助检测试剂，这些试剂现与B42 Antibody (E-2) 打包提供（请参阅下面的订购信息）。

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    描述
    说明书与实验方案
    研究信息

    関連項目

    B42抗体（E-2）是一种IgG1 κ小鼠单克隆B42抗体（也称为B42抗体），可通过WB, IP, IF 和 ELISA检测B42蛋白。B42抗体（E-2）既有非偶联的抗B42抗体形式，也有多种偶联形式的抗B42抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。酿酒酵母的GAL4蛋白是最具特征的转录激活因子之一。双杂交系统是一种广泛用于检测蛋白质-蛋白质相互作用的方法，它利用基因工程酵母或细菌。它涉及一个与DNA结合域（DBD）融合的"诱饵"蛋白和一个与转录激活域（AD）连接的"猎物"蛋白，从而在它们相互作用时激活报告基因。该系统中常用的DBD包括GAL4或LexA，而AD通常来自GAL4或单纯疱疹病毒蛋白VP16。此外，B42蛋白也用作替代AD，特别是在使用LexA DBD的配置中。该系统已发展到包括原核蛋白，如大肠杆菌LexA的DBD，以及酿酒酵母GAL4的N端，后者以其DNA结合能力而闻名。VP16或B42的AD的加入提供了强大的转录激活，增强了该系统在不同生物体中的通用性。这种适应性，加上特异性激活转录和确保蛋白质在细胞内适当定位的能力，使双杂交系统成为蛋白质组学研究和相互作用组映射的首选。

    仅限研究使用。不适用于诊断和治疗用途。

    Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

    LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

    B42 抗体 (E-2) 参考文献:

    1. 用于在酵母或哺乳动物细胞中表达的 GAL4 融合载体。  |  Sadowski, I., et al. 1992. Gene. 118: 137-41. PMID: 1511877
    2. 双杂交系统：一种识别和克隆与感兴趣的蛋白质相互作用的蛋白质基因的方法。  |  Chien, CT., et al. 1991. Proc Natl Acad Sci U S A. 88: 9578-82. PMID: 1946372
    3. 酵母 STE12 蛋白的信息素依赖性磷酸化与转录激活有关。  |  Song, D., et al. 1991. Genes Dev. 5: 741-50. PMID: 2026326
    4. 激活剂和目标。  |  Ptashne, M. and Gann, AA. 1990. Nature. 346: 329-31. PMID: 2142753
    5. 检测蛋白质-蛋白质相互作用的新型基因系统。  |  Fields, S. and Song, O. 1989. Nature. 340: 245-6. PMID: 2547163
    6. 真菌基因调控机制模型：酿酒酵母的 GAL 基因。  |  Johnston, M. 1987. Microbiol Rev. 51: 458-76. PMID: 2830478
    7. GAL4 的缺失分析确定了两个转录激活区段。  |  Ma, J. and Ptashne, M. 1987. Cell. 48: 847-53. PMID: 3028647

    订购信息

    产品名称产品编号规格价格数量收藏夹

    B42 抗体 (E-2)

    sc-377101
    200 µg/ml
    		RMB2377.00

    B42 (E-2): m-IgG Fc BP-HRP 套装

    sc-527580
    200 µg Ab; 10 µg BP
    		RMB2662.00

    B42 (E-2): m-IgG1 BP-HRP 套装

    sc-532953
    200 µg Ab; 20 µg BP
    		RMB2662.00

    B42 抗体 (E-2) AC

    sc-377101 AC
    500 µg/ml, 25% agarose
    		RMB3129.00

    B42 抗体 (E-2) HRP

    sc-377101 HRP
    200 µg/ml
    		RMB2377.00

    B42 抗体 (E-2) FITC

    sc-377101 FITC
    200 µg/ml
    		RMB2482.00

    B42 抗体 (E-2) PE

    sc-377101 PE
    200 µg/ml
    		RMB2580.00

    B42 抗体 (E-2) Alexa Fluor® 488

    sc-377101 AF488
    200 µg/ml
    		RMB2685.00

    B42 抗体 (E-2) Alexa Fluor® 546

    sc-377101 AF546
    200 µg/ml
    		RMB2685.00

    B42 抗体 (E-2) Alexa Fluor® 594

    sc-377101 AF594
    200 µg/ml
    		RMB2685.00

    B42 抗体 (E-2) Alexa Fluor® 647

    sc-377101 AF647
    200 µg/ml
    		RMB2685.00

    B42 抗体 (E-2) Alexa Fluor® 680

    sc-377101 AF680
    200 µg/ml
    		RMB2685.00

    B42 抗体 (E-2) Alexa Fluor® 790

    sc-377101 AF790
    200 µg/ml
    		RMB2685.00

    B42 (E-2) 中和勝肽

    sc-377101 P
    100 µg/0.5 ml
    		RMB511.00

    What application is the blocking peptide sc-377101 P appropriate for?

    Asked by: TinTin
    Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
    Answered by: Technical Support
    Date published: 2023-12-13
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    Rated 5 out of 5 by from Great featuresI used this antibody to do the experiment, get a good effect, very beautiful data
    Date published: 2024-09-13
    Rated 5 out of 5 by from Good productI brought this product a month ago and i am so happy that i did it
    Date published: 2024-08-20
    Rated 5 out of 5 by from the good protein-protein interactionsThis approach is facilitated by the finding that higher eukaryotes lack endogenous proteins that enhance transcription from the consensus GAL4-binding site. Fusions between GAL4 (aa 1-147) and activating domains from a variety of transcriptional regulatory proteins can activate transcription in yeast, plant, insects and mammalian cells. A unique “two-hybrid” system has been developed using GAL4 fusions in yeast to identify specific protein-protein interactions.
    Date published: 2017-02-01
    Rated 5 out of 5 by from Great antibody for WBI used this antibody to do the experiment, get a good effect, very beautiful data,in WB in,at 1:500 dilution.
    Date published: 2017-01-26
    Rated 5 out of 5 by from excellentWestern blot data of full-length B42 fusion protein is very clear.
    Date published: 2016-11-06
    Rated 5 out of 5 by from Good dataI used B42 a month ago and it provided excellent WB data.
    Date published: 2016-11-05
    Rated 5 out of 5 by from Good Western blot data of fullGood Western blot data of full-length B42 fusion protein -SCBT QC
    Date published: 2013-03-29
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    B42 抗体 (E-2) is rated 5.0 out of 5 by 7.
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