Date published: 2025-9-8
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Axin Antibody (2B11) - Immunofluorescence - Image 346130
Axin 抗体 (2B11): sc-293190. 甲醇固定的 HeLa 细胞免疫荧光染色, 显示胞质, 膜和核定位.
Axin Antibody (2B11) - Immunofluorescence - Image 346130
Axin 抗体 (2B11): sc-293190. 甲醇固定的 HeLa 细胞免疫荧光染色, 显示胞质, 膜和核定位.
Axin Antibody (2B11) - Western Blotting - Image 348894
Axin 抗体 (2B11): sc-293190. 人重组 Axin 融合蛋白的 Western 印迹分析.
Axin 抗体 (2B11): sc-293190. 甲醇固定的 HeLa 细胞免疫荧光染色, 显示胞质, 膜和核定位.

Axin 抗体 (2B11): sc-293190

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寫評論提問

说明书
  • Axin 抗体 2B11 是小鼠单克隆 IgG2a κ，Axin抗体, 在11篇文献中引用，规格为100 µg/ml
  • 免疫human物种的Axin的氨基酸643-740
  • 推荐用于 human 来源的Axin WB, IP, IF, IHC(P) 和 ELISA检测
  • 关于如何获取Axin (2B11): sc-293190的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG2a BP-HRP 是 Axin 抗体 (2B11) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Axin 抗体 (2B11) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

Axin 抗体（2B11）是一种小鼠单克隆 IgG2a kappa 轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、免疫组织化学和酶联免疫吸附测定（ELISA）检测人类 Axin 蛋白。Anti-Axin 抗体（2B11）以非结合形式提供。Axin在调节Wnt信号通路中起着至关重要的作用，而Wnt信号通路对于细胞增殖、分化以及胚胎发育等各种细胞过程至关重要。Axin作为支架，促进包括β-catenin、腺瘤性息肉病（APC）和糖原合酶激酶3β（GSK-3β）在内的复合物的形成，从而促进β-catenin降解。这种降解可防止异常的Wnt信号传导，后者会导致细胞无序生长和癌症。Axin的结构完整性使其能够通过多个结合域实现有效的蛋白质-蛋白质相互作用，从而在信号传导通路中实现有效的伴侣结合。Axin与其他蛋白质（例如与Axin具有45%同源性的Conductin）之间的相互作用凸显了Axin在维持Wnt信号传导平衡以及肿瘤发生中的重要性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Axin 抗体 (2B11) 参考文献:

  1. Axin与癌变之间的联系。  |  Salahshor, S. and Woodgett, JR. 2005. J Clin Pathol. 58: 225-36. PMID: 15735151
  2. 抑制 Tankyrase 可稳定轴突蛋白并颉颃 Wnt 信号作用。  |  Huang, SM., et al. 2009. Nature. 461: 614-20. PMID: 19759537
  3. 对Axin在典型Wnt信号通路中的功能调节的新见解。  |  Song, X., et al. 2014. Protein Cell. 5: 186-93. PMID: 24474204
  4. Axin在大脑皮层发育中的新兴作用。  |  Ye, T., et al. 2015. Front Cell Neurosci. 9: 217. PMID: 26106297
  5. 轴突蛋白在生物分子凝聚物中的磷酸化可抵消其由毒蕈碱酶介导的降解。  |  Klement, K., et al. 2023. J Cell Sci. 136: PMID: 37721093
  6. R-spondin-1通过LRP6-CK1ε轴诱导Axin降解。  |  Tan, L., et al. 2024. Cell Commun Signal. 22: 14. PMID: 38183076
  7. APC突变破坏了由Axin相分离组织的β-catenin破坏复合物凝结物。  |  Zhang, D., et al. 2024. Cell Mol Life Sci. 81: 57. PMID: 38279052
  8. E-cadherin 和 APC 竞争与 beta-catenin 和细胞骨架的相互作用。  |  Hülsken, J., et al. 1994. J Cell Biol. 127: 2061-9. PMID: 7806582
  9. beta-catenin 与转录因子 LEF-1 的功能相互作用。  |  Behrens, J., et al. 1996. Nature. 382: 638-42. PMID: 8757136
  10. 小鼠 Fused 基因座编码 Axin，它是 Wnt 信号通路的阻抑剂，可调节胚胎轴的形成。  |  Zeng, L., et al. 1997. Cell. 90: 181-92. PMID: 9230313
  11. beta-catenin 是泛素-蛋白酶体途径的靶标。  |  Aberle, H., et al. 1997. EMBO J. 16: 3797-804. PMID: 9233789
  12. 轴突蛋白同源物传导蛋白与 beta-catenin, APC 和 GSK3beta 的功能相互作用  |  Behrens, J., et al. 1998. Science. 280: 596-9. PMID: 9554852

订购信息

产品名称产品编号规格价格数量收藏夹

Axin 抗体 (2B11)

sc-293190
100 µg/ml
RMB2377.00

Axin (2B11): m-IgG Fc BP-HRP 套装

sc-540275
100 µg Ab; 10 µg BP
RMB2662.00

Axin (2B11): m-IgG2a BP-HRP 套装

sc-546880
100 µg Ab; 10 µg BP
RMB2662.00

Does this antibody recognise Axin1 or Axin2?

Asked by: Anatomical Science
Thank you for the question. This antibody recognizes Axin1. It was raised against human AXIN1 amino acids 643-740 from protein accession number O15169. The Sequence was ISRHRRTGHGSSGTRKPQPHENSRPLSLEHPWAGPQLRTSVQPSHLFIQDPTMPPHPAPNPLTQLEEARRRLEEEEKRASRAPSKQRYVQEVMRRGRA.
Answered by: Technical Support Europe
Date published: 2018-01-09

Can sc-293190: Axin (2B11) monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: Trav11
Thank you for your question. Yes, sc-293190: Axin (2B11) is recommended for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 4 out of 5 by from Great featureI used this antibody in ovarian cancer cells in EMT. It works very well. Good signal at the right size. Dilution used: (1/300 to 1/500)
Date published: 2018-11-22
Rated 5 out of 5 by from fluorescent ICCI got good immunofluorescent images of paraformaldehyde-fixed mouse embryonic fibloblast (MEF) cells using axin (2B11) antibody under confocal microscope. Axin is well distributed in all cytoplasm except nucleus. It works very well
Date published: 2018-02-01
Rated 4 out of 5 by from does detect endogenous Axin in HEK293Additional bands at 60,45, 30 and 15kDa when HEK293 is used
Date published: 2018-01-03
Rated 5 out of 5 by from Western blot looks niceAt dilution of 1:500, this antibody worked in overexpressed HEK cells. Although not included, we do not see a good bad in non overexpressed HEK cells loaded at 25ug lysates protein. But the immunofluoresence used at 1:50 works really well in HEK non overexpressed cells.
Date published: 2017-12-12
Rated 5 out of 5 by from specific resultThis antibody is very strong, accurate. I like it. The Axin antibody doesn't requires any optimisation,just use the dilution 1:500, It is highly specific for the targett protein.
Date published: 2017-07-22
Rated 5 out of 5 by from very efficientI am very glad that this antibody is very efficient. I used Axin antibody of 1:500 dilution in WB.,loaded 60 ug.
Date published: 2017-05-23
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Axin 抗体 (2B11) is rated 4.7 out of 5 by 6.
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