Date published: 2025-9-8
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ATP5A Antibody (51) - Western Blotting - Image 387333
ATP5A 抗体 (51): sc-136178. HeLa (A), Jurkat (B) 和 ZR-75-1 (C) 全细胞裂解液及小鼠胚胎心脏组织提取物 (D) 中 ATP5A 表达的 Western 印迹分析.
ATP5A Antibody (51) - Western Blotting - Image 387333
ATP5A 抗体 (51): sc-136178. HeLa (A), Jurkat (B) 和 ZR-75-1 (C) 全细胞裂解液及小鼠胚胎心脏组织提取物 (D) 中 ATP5A 表达的 Western 印迹分析.
ATP5A Antibody (51) - Western Blotting - Image 128017
ATP5A 抗体 (51): sc-136178. 对 HeLa 全细胞裂解液中 ATP5A 的表达进行 Western 印迹分析.
ATP5A Antibody (51) - Western Blotting - Image 412875
ATP5A 抗体 （51）： sc-136178. Western blot 分析 HeLa （A）、K-562 （B） 和 HCT-116 （C） 细胞裂解液中 ATP5A 的表达。检测试剂：m-IgG Fc BP-HRP： sc-525409.
ATP5A 抗体 (51): sc-136178. HeLa (A), Jurkat (B) 和 ZR-75-1 (C) 全细胞裂解液及小鼠胚胎心脏组织提取物 (D) 中 ATP5A 表达的 Western 印迹分析.

ATP5A 抗体 (51): sc-136178

4.7(15)
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说明书
  • ATP5A 抗体 51 是小鼠单克隆 IgG2a κ，ATP5A抗体, 在40篇文献中引用，规格为200 µg/ml
  • 免疫human物种的ATP5A的氨基酸113-220
  • 推荐用于 mouse, rat， human 和 Arabidopsis thaliana 来源的ATP5A WB, IP 和 IF检测; 也和以下物种反应，包括： canine
  • 关于如何获取ATP5A (51): sc-136178的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP和 2a BP-HRP">m-IgG2a BP-HRP是ATP5A Antibody (51) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与ATP5A Antibody (51) 打包提供（请参阅下面的订购信息）。

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描述
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研究信息

関連項目

额外ATP5抗体，包括ATP7, ATP10, ATP13, ATP5, ATP6, ATP5A, ATP5B, ATP5E, ATP5F1, ATP5G1和ATP5H

ATP5A抗体（51）是一种IgG2a κ小鼠单克隆ATP5A抗体，可通过WB, IP和IF检测小鼠、大鼠、人类和拟南芥来源的ATP5A蛋白。ATP5A抗体（51）可作为非偶联的抗ATP5A抗体。线粒体ATP合酶（ATPases）将膜电化学质子梯度中的能量转化为合成高能磷酸键所需的能量。ATPases包含两个连接的复合物：F1，亲水性催化核心；以及F0，膜嵌入的蛋白质通道。F1由三条α链和三条β链组成，它们之间弱同源，以及一条γ链、一条δ链和一条ε链。F0由三个亚基组成：a、b和c。F1的α链是一个含有509个氨基酸的调节亚基。线粒体ATPase α链（ATP5A）定位于线粒体并催化ATP的合成。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

ATP5A 抗体 (51) 参考文献:

  1. ATP合酶α链与阿尔茨海默病神经纤维变性有关。  |  Sergeant, N., et al. 2003. Neuroscience. 117: 293-303. PMID: 12614671
  2. 人类线粒体 ATP 合酶 alpha 亚基 cDNA 的核苷酸序列。  |  Kataoka, H. and Biswas, C. 1991. Biochim Biophys Acta. 1089: 393-5. PMID: 1830491
  3. β-淀粉样肽结合并调节神经表面异位ATP合酶α链。  |  Xing, SL., et al. 2012. Int J Neurosci. 122: 290-7. PMID: 22185089
  4. 牛线粒体 F1-ATP 酶的一级结构和亚基计算公式  |  Walker, JE., et al. 1985. J Mol Biol. 184: 677-701. PMID: 2864455
  5. 在骨骼发育过程中，线粒体功能的维持需要 RGS12。  |  Yuan, G., et al. 2020. Cell Discov. 6: 59. PMID: 32922858
  6. LINC00467编码的微肽ASAP通过直接调节ATP合酶活性促进结直肠癌进展。  |  Ge, Q., et al. 2021. J Clin Invest. 131: PMID: 34591791
  7. TNK2/ACK1 介导的 ATP5F1A（ATP 合酶 F1 亚基α）磷酸化选择性地提高了前列腺癌的存活率，同时造成线粒体的脆弱性。  |  Chouhan, S., et al. 2023. Autophagy. 19: 1000-1025. PMID: 35895804
  8. 新型 ATP 合酶复合物调节剂 MTA1 通过线粒体代谢重编程促进结肠癌肝转移  |  Wang, T., et al. 2023. Adv Sci (Weinh). 10: e2300756. PMID: 37442756
  9. 阿托伐他汀通过WWP2介导的ATP5A泛素化和降解，修复高血压引起的血管内皮损伤。  |  Yin, Z., et al. 2023. Biomed Pharmacother. 166: 115228. PMID: 37557013
  10. 蛋白质组学分析发现，与匹配的原发性肿瘤相比，HK1和ATP5A在肺腺癌的远处转移中过度表达。  |  Pasternack, H., et al. 2023. Sci Rep. 13: 20948. PMID: 38016997
  11. 嗜热芽孢杆菌 PS3 的 F1-ATP 酶无核苷酸的 alpha 3 beta 3 亚复合物的晶体结构为对称三聚体。  |  Shirakihara, Y., et al. 1997. Structure. 5: 825-36. PMID: 9261073
  12. Y79的比较基因组杂交分析和FISH图谱显示，扩增的人类线粒体ATP合成酶α亚基基因（ATP5A）映射到染色体18q12-->q21上。  |  Godbout, R., et al. 1997. Cytogenet Cell Genet. 77: 253-6. PMID: 9284928

订购信息

产品名称产品编号规格价格数量收藏夹

ATP5A 抗体 (51)

sc-136178
200 µg/ml
RMB2377.00

ATP5A (51): m-IgG Fc BP-HRP 套装

sc-539899
200 µg Ab; 10 µg BP
RMB2662.00

ATP5A (51): m-IgG2a BP-HRP 套装

sc-546814
200 µg Ab; 10 µg BP
RMB2662.00

Is there any information whether anti-ATP5A(51) also recognizes chloroplastic ATPA sequence in Arabidopsis thaliana?

Asked by: HCAC
Thank you for your question. This antibody has not yet been tested in Arabidopsis and so that is not a recommended species at this time. We are still gathering data to show on our website. Check back for updates!
Answered by: Tech Service
Date published: 2020-03-16

For Western Blot, is it recommended to use denatured or non-denatured conditions with ATP5A (51): sc-136178 antibody?

Asked by: TinTin
Thank you for your question. We recommend this antibody for use in denatured Western Blot conditions. It has not been validated for use in non-denatured conditions. Please contact our Technical Service Department for further details or inquiries.
Answered by: Technical Support
Date published: 2021-07-22
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Rated 5 out of 5 by from subunidad del complejo V de la cadena respiratoriaMe dieron una muestra y nos funciono a la perfección y por eso ya lo compramos.
Date published: 2024-03-20
Rated 5 out of 5 by from excellent strong antibody!!!I performed checking H9c2 cell lysate. Very good. I expect good results in isolated mito-fraction.
Date published: 2022-06-29
Rated 5 out of 5 by from Great features.We used antibody for the western blot (1:500) in nonsynaptic mitochondrial fractions in different samples of rat brain hippocampus and got clear bands.
Date published: 2020-12-29
Rated 5 out of 5 by from Bright signal, No backgroundAntibody works greatly on WB at dilution 1:500. Totally Recommended!
Date published: 2020-01-15
Rated 5 out of 5 by from Works wellIt worked at a concentration of 1:2000, without loss of signals.
Date published: 2019-10-15
Rated 4 out of 5 by from Works wellwe Got a free sample and it works well for Western blot.
Date published: 2019-03-01
Rated 4 out of 5 by from Works fairly well on yeast atp5a (atp1)In S. cerevisiae, I used 1:1000 dilution and overnight incubation in 5%milk in TBST, followed by 1:5000 goat anti-mouse for 1hr at room temp. Had to expose for 5mins to get any signal so the binding is a bit weak but the band was clean and at the right molecular weight.
Date published: 2018-11-02
Rated 5 out of 5 by from Works great without any backgroundWorks great without any background in DC 2.4 cells for WB at 1:200
Date published: 2017-12-13
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ATP5A 抗体 (51) is rated 4.7 out of 5 by 15.
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