Date published: 2025-9-7

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ATF-2 抗体 (F2BR-1): sc-242

4.5(14)
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说明书
  • ATF-2 抗体 F2BR-1 是小鼠单克隆 IgG1 κ,ATF-2抗体, 在86篇文献中引用,规格为200 µg/ml
  • 针对氨基酸350-505的抗原,在human的ATF-2的DNA结合和二聚化结构域内
  • ATF-2 抗体 (F2BR-1) 推荐用于 WB, IP 和 IF,检测mouse, rat 和human 来源的 ATF-2
  • 抗ATF-2抗体(F2BR-1)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-242 X, 200 µg/0.1 ml)
  • 关于如何获取ATF-2 (F2BR-1): sc-242免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP1 BP-HRP">m-IgG1 BP-HRPm-IgGκ BP-HRP是ATF-2 Antibody (F2BR-1) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与ATF-2 Antibody (F2BR-1) 打包提供(请参阅下面的订购信息)。

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関連項目

ATF-2抗体(F2BR-1)是一种小鼠单克隆IgG1 kappa轻链抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)和免疫荧光(IF)检测小鼠、大鼠和人类来源的ATF-2蛋白。ATF-2 (F2BR-1) 抗体有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 结合物。ATF-2蛋白在调节基因表达方面发挥着至关重要的作用,尤其是对压力信号的反应,它作为转录因子与特定的DNA序列结合,称为cAMP反应元件(CRE)。这种结合对于参与细胞应激反应、细胞增殖和分化的基因的转录激活至关重要。ATF-2可以与其他蛋白质形成同源二聚体或异源二聚体,例如c-Jun,从而增强转录调控能力。ATF-2经历翻译后修饰,包括特定苏氨酸残基的磷酸化,这对激活和稳定性至关重要。ATF-2在Thr 69和Thr 71处的磷酸化由应激激活的激酶进行,这对转录活性尤为重要,而Myc诱导的磷酸化也有助于稳定性。ATF-2具有组蛋白乙酰转移酶活性,特别是乙酰化组蛋白H2B和H4,从而影响染色质结构和基因可及性。ATF-2的多重作用强调了其在细胞对环境信号的反应中的重要性,以及对包括癌症和神经退行性疾病在内的各种疾病的潜在影响。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

ATF-2 抗体 (F2BR-1) 参考文献:

  1. ATF-2 具有固有的组蛋白乙酰转移酶活性,可通过磷酸化进行调节。  |  Kawasaki, H., et al. 2000. Nature. 405: 195-200. PMID: 10821277
  2. Myc 与激活转录因子 2(ATF2)之间的相互影响:Myc 可延长 ATF2 的半衰期并诱导其磷酸化。  |  Miethe, J., et al. 2001. Oncogene. 20: 8116-24. PMID: 11781825
  3. ATF-2在肿瘤发生中的作用。  |  Vlahopoulos, SA., et al. 2008. Bioessays. 30: 314-27. PMID: 18348191
  4. 将环磷酸腺苷反应元件结合蛋白 2 (CREB2) 的基因归入人类染色体 2q24.1-q32。  |  Diep, A., et al. 1991. Genomics. 11: 1161-3. PMID: 1838349
  5. 转录因子 ATF cDNA 克隆:能够选择性地形成 DNA 结合异二聚体的大量亮氨酸拉链蛋白家族。  |  Hai, TW., et al. 1989. Genes Dev. 3: 2083-90. PMID: 2516827
  6. 有丝分裂后和终末分化的人牙本质细胞中的ATF-2免疫反应。  |  Keklikoglu, N. and Akinci, S. 2015. Med Mol Morphol. 48: 164-8. PMID: 25417007
  7. 一种常见的细胞转录因子 ATF 与 E1a 和环 AMP 诱导的启动子中的调控元件相互作用。  |  Lin, YS. and Green, MR. 1988. Proc Natl Acad Sci U S A. 85: 3396-400. PMID: 2835770
  8. 鉴定大鼠体生长抑素基因中的环-AMP 反应元件。  |  Montminy, MR., et al. 1986. Proc Natl Acad Sci U S A. 83: 6682-6. PMID: 2875459
  9. ERK1/ATF-2信号轴促进皮肤成纤维细胞中白细胞介素-1β诱导的MMP-3表达。  |  Kitanaka, N., et al. 2019. PLoS One. 14: e0222869. PMID: 31536594
  10. 激活转录因子-2(ATF-2)的过度表达激活Wnt/Ca2+信号通路,促进非小细胞肺癌的增殖和侵袭。  |  Zhang, L., et al. 2022. Dis Markers. 2022: 5772089. PMID: 35692887
  11. ATF-2 优先被应激活化蛋白激酶激活,在基因毒性物质作用下介导 c-jun 诱导。  |  van Dam, H., et al. 1995. EMBO J. 14: 1798-811. PMID: 7737130
  12. cJun 和 ATF-2 的异源二聚体形成是 243 氨基酸腺病毒 E1A 蛋白诱导 c-jun 的原因。  |  van Dam, H., et al. 1993. EMBO J. 12: 479-87. PMID: 8382609

订购信息

产品名称产品编号规格价格数量收藏夹

ATF-2 抗体 (F2BR-1)

sc-242
200 µg/ml
RMB2377.00

ATF-2 (F2BR-1): m-IgG Fc BP-HRP 套装

sc-528101
200 µg Ab; 10 µg BP
RMB2662.00

ATF-2 (F2BR-1): m-IgGκ BP-HRP 套装

sc-520399
200 µg Ab, 40 µg BP
RMB2662.00

ATF-2 (F2BR-1): m-IgG1 BP-HRP 套装

sc-542759
200 µg Ab; 20 µg BP
RMB2662.00

ATF-2 抗体 (F2BR-1) X

sc-242 X
200 µg/0.1 ml
RMB2377.00

ATF-2 抗体 (F2BR-1) AC

sc-242 AC
500 µg/ml, 25% agarose
RMB3129.00

ATF-2 抗体 (F2BR-1) HRP

sc-242 HRP
200 µg/ml
RMB2377.00

ATF-2 抗体 (F2BR-1) FITC

sc-242 FITC
200 µg/ml
RMB2482.00

ATF-2 抗体 (F2BR-1) PE

sc-242 PE
200 µg/ml
RMB2580.00

ATF-2 抗体 (F2BR-1) Alexa Fluor® 488

sc-242 AF488
200 µg/ml
RMB2685.00

ATF-2 抗体 (F2BR-1) Alexa Fluor® 546

sc-242 AF546
200 µg/ml
RMB2685.00

ATF-2 抗体 (F2BR-1) Alexa Fluor® 594

sc-242 AF594
200 µg/ml
RMB2685.00

ATF-2 抗体 (F2BR-1) Alexa Fluor® 647

sc-242 AF647
200 µg/ml
RMB2685.00

ATF-2 抗体 (F2BR-1) Alexa Fluor® 680

sc-242 AF680
200 µg/ml
RMB2685.00

ATF-2 抗体 (F2BR-1) Alexa Fluor® 790

sc-242 AF790
200 µg/ml
RMB2685.00

When should the TransCruz reagent sc-242 X be used instead of the standard form sc-242?

Asked by: AbPolly
Thank you for your question. We recommend using the TransCruz reagent form when performing ChIP or GelShift assays. This form can also be used in other applications such as Western Blot or IHC, but the dilution should be adjusted by a factor of 10 to account for the higher original concentration.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Good ATF AntibodyI used the sample antibody and obtained clean WB results in HEK-293T cells.
Date published: 2017-08-16
Rated 5 out of 5 by from Great antibodyOur lab likes to use this antibody because the product has a strong band and clean background.
Date published: 2017-05-25
Rated 5 out of 5 by from Antibody quality is very good.Antibody quality is very good.I will introduce the with partners.
Date published: 2017-04-28
Rated 5 out of 5 by from quality is very goodProduct quality is very good. It help me a lot.I will continue to support and buy this antibody for WB detection.
Date published: 2017-04-15
Rated 5 out of 5 by from This antibody works well.ATF-2 antibody the analysis results of Western blot were very strong and clear.This antibody works well.
Date published: 2017-02-07
Rated 4 out of 5 by from Favorite antibodyWe use this antibody to get a strong band and clean background.
Date published: 2017-02-04
Rated 5 out of 5 by from Excellent productThe ATF-2 Antibody (F2BR-1): sc-242 provides great data for Western blot applications, showing clear banding with minimal background. This antibody has also been cited over 50 times for further verification.
Date published: 2017-02-02
Rated 5 out of 5 by from I am very satisfied.My strong point is to do the western blot experiment, six months ago, I bought this antibody, always been preserved for 4°,1:300 dilution, still can run out of good results, I am very satisfied.
Date published: 2016-12-03
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ATF-2 抗体 (F2BR-1) is rated 4.5 out of 5 by 14.
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