Date published: 2026-10-24

1-800-457-3801

SCBT Portrait Logo
Seach Input
联系我们
登陆账户
快速订购
购物车 (0)
ATF-1/ATF1 Antibody (C41-5.1) - Western Blotting - Image 374018
ATF-1 抗体 (C41-5. 1): sc-243. 在 HeLa (A) 和 F9 (B) 全细胞裂解液以及 NIH/3T3 (C) 和 K-562 (D) 核提取物中 ATF-1 表达的 Western 印迹分析.
ATF-1 抗体 (C41-5. 1): sc-243. 在 HeLa (A) 和 F9 (B) 全细胞裂解液以及 NIH/3T3 (C) 和 K-562 (D) 核提取物中 ATF-1 表达的 Western 印迹分析.

ATF-1/ATF1 抗体 (C41-5.1): sc-243

4.8(10)
寫評論提問
说明书
  • ATF-1/ATF1抗体(C41-5.1)是小鼠单克隆IgA κ, 在104篇文献中引用，规格为200 µg/ml
  • 免疫human物种的重组ATF-1
  • 推荐用于 mouse, rat 和 human 来源的ATF-1 p35 WB 和 IP检测
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-243 X, 200 µg/0.1 ml)
  • 看 ATF-1/ATF1 (25C10G): sc-270，可查看有标记的 ATF-1/ATF1 抗体，包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • 目前，我们还没有完成ATF-1/ATF1 抗体 (C41-5.1)的首选二抗检测试剂的鉴定。这项工作正在进行中。
Gene Editing Promo Banner

快捷链接

相关产品
描述
基因信息
说明书与实验方案
研究信息

関連項目

额外ATF抗体，包括ATF-1, ATF-2, ATF-3, ATF-5, ATF-6 α, ATF-5 β和ATF-7IP

ATF-1/ATF1抗体（C41-5.1）是一种IgAκ小鼠单克隆ATF-1抗体，可通过WB和IP检测小鼠、大鼠和人类来源的ATF-1 p35。ATF-1/ATF1抗体（C41-5.1）是非偶联的抗ATF-1抗体形式提供。真核基因转录受到序列特异性转录因子结合模块化的cis作用启动子和增强子元件的调控。ATF/CREB转录因子家族结合了回文cAMP应答元件（CRE）八核苷酸序列TGACGTCA。ATF/CREB家族包括CREB-1、CREB-2（也称为ATF-4）、ATF-1、ATF-2和ATF-3。这个蛋白质家族具有高度不同的N末端结构，但共享C末端的亮氨酸拉链以便于二聚体形成和DNA结合。ATF-1被证明在NOX1诱导中起着关键作用。ATF-1结合cAMP应答元件（CRE），介导PKA诱导的CRE报告基因激活。ATF-2形成同源二聚体和与c-Jun的异源二聚体，以启动CRE依赖的转录。应激激活激酶磷酸化ATF-2的Thr 69和Thr 71是转录激活所必需的。Myc也诱导ATF-2在Thr 69和Thr 71的磷酸化，以延长ATF-2的半衰期。ATF-2作为组蛋白乙酰转移酶（HAT），在体外特异性乙酰化组蛋白H2B和H4。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

ATF-1/ATF1 抗体 (C41-5.1) 参考文献:

  1. 腺病毒 12 型 E2 启动子的 cAMP 非依赖性激活与 CREB-1/ATF-1, E1A(12S) 和 CBP 招募到 E2-CRE 相关。  |  Fax, P., et al. 2000. J Biol Chem. 275: 8911-20. PMID: 10722738
  2. 霍奇金病分离株中爱泼斯坦-巴尔病毒（EBV）潜伏膜蛋白 1（LMP-1）基因和启动子的分析：针对 LMP-1 启动子 ATF-1/CREB-1 结合位点发生突变的 EBV 变种的选择。  |  Sandvej, K., et al. 2000. Mol Pathol. 53: 280-8. PMID: 11091852
  3. 软组织透明细胞肉瘤中的 EWS-ATF-1 嵌合蛋白与 CREB 结合蛋白结合，干扰 p53 介导的转激活功能。  |  Fujimura, Y., et al. 2001. Oncogene. 20: 6653-9. PMID: 11709699
  4. ATF-1 在血管平滑肌细胞中介导蛋白酶激活受体-1，而非受体酪氨酸激酶诱导的 DNA 合成。  |  Ghosh, SK., et al. 2002. J Biol Chem. 277: 21325-31. PMID: 11925444
  5. EWS/ATF-1嵌合转录因子的靶基因鉴定。  |  Jishage, M., et al. 2003. Oncogene. 22: 41-9. PMID: 12527906
  6. cAMP调节的增强子结合蛋白ATF-1激活转录，以响应cAMP依赖性蛋白激酶A。  |  Rehfuss, RP., et al. 1991. J Biol Chem. 266: 18431-4. PMID: 1655749
  7. ATF-1转录因子在OS-2 MAP激酶作用下调控ccg-1和cat-1基因在十字花科神经孢子属中对氟虫腈的表达  |  Yamashita, K., et al. 2008. Fungal Genet Biol. 45: 1562-9. PMID: 18948219
  8. 转录因子 ATF-1 和 CREB-1 与缺氧诱导因子-1（HIF-1）的 DNA 识别位点构成性结合。  |  Kvietikova, I., et al. 1995. Nucleic Acids Res. 23: 4542-50. PMID: 8524640
  9. EWS-ATF-1基因涉及染色体t（12；22）易位的软组织恶性黑色素瘤，该基因编码一种组成型转录激活因子。  |  Fujimura, Y., et al. 1996. Oncogene. 12: 159-67. PMID: 8552387
  10. 牛白血病病毒感染的 B 淋巴细胞中的 CREB, ATF-1 和 ATF-2 转录因子可激活病毒表达。  |  Adam, E., et al. 1996. J Virol. 70: 1990-9. PMID: 8627725

订购信息

产品名称产品编号规格价格数量收藏夹

ATF-1/ATF1 抗体 (C41-5.1)

sc-243
200 µg/ml
CNY2422.00

ATF-1/ATF1 抗体 (C41-5.1) X

sc-243 X
200 µg/0.1 ml
CNY2422.00

When should the TransCruz reagent sc-243 X be used instead of the standard form sc-243?

Asked by: jerojero
Thank you for your question. We recommend using the TransCruz reagent form when performing ChIP or GelShift assays. This form can also be used in other applications such as Western Blot or IHC, but the dilution should be adjusted by a factor of 10 to account for the higher original concentration.
Answered by: Technical Support
Date published: 2017-02-24
  • y_2026, m_10, d_23, h_6CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.48
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_1
  • loc_zh_CN, sid_243, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 85ms
  • QUESTIONS, PRODUCT
Rated 4 out of 5 by Julia123321 from It is fine.Used for WB to detect human cancers. Got the specific band, but too weak. Supersignal ECL is required.
Date published: 2020-08-13
Rated 4 out of 5 by Newt1 from Specific for ATF1 in WBI overexpressed GFP-tagged ATF1, CREB and CREM in HEK293 cells. ATF1 C41-5.1 was specific to ATF1 in WB, as it recognised only GFP-ATF1 protein, but not GFP-CREB or GFP-CREM. Dilution 1:200 worked very well in WB with overexpressed ATF1 in HEK293 cells, but failed to recognize endogenous ATF1 and gave a very strong background signal in rat primary cortical neuron lysates.
Date published: 2018-08-23
Rated 5 out of 5 by DefinitelyNotMatt from Great Antibody for WBThis antibody is well-cited for a variety of applications including WB, IP, even ChIP. Strong, clear signal with little to no background. Works well in a variety of species including mouse, rat, and human samples.
Date published: 2017-01-30
Rated 5 out of 5 by V_G_ from Used this antibody for WB detection and itUsed this antibody for WB detection and it worked well.
Date published: 2015-10-05
Rated 5 out of 5 by QZ from Strong band in WB in HeLa nuclear extractStrong band in WB in HeLa nuclear extract. -SCBT QC
Date published: 2015-04-06
Rated 5 out of 5 by MC from Data published for WBData published for WB, IP, and EMSA in rat cortical neuron. -SCBT Publication Review
Date published: 2015-03-31
Rated 5 out of 5 by HC from Published data for WB in mouse RAW264Published data for WB in mouse RAW264.7 and human THP-1 cell lines. -SCBT Publication Review
Date published: 2015-03-27
Rated 5 out of 5 by FL from Publishable data for EMSA in human JEGPublishable data for EMSA in human JEG-3, JAR, Tera-2, and HeLa cells. -SCBT Publication Review
Date published: 2015-03-17
  • y_2026, m_10, d_23, h_6
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.48
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_10
  • loc_zh_CN, sid_243, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 10ms
  • REVIEWS, PRODUCT
ATF-1/ATF1 抗体 (C41-5.1) is rated 4.8 out of 5 by 10.
  • y_2026, m_10, d_23, h_6
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.48
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_10
  • loc_zh_CN, sid_243, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 98ms
  • REVIEWS, PRODUCT
1-800-457-3801
访问 Santa Cruz Animal Health|访问 San Juan Ranch
Santa Cruz Biotechnology, Inc. 是生物医学研究市场产品开发的世界领导者。请拨打1-800-457-3801联系我们。
版权所有© 2007-2026 Santa Cruz Biotechnology, Inc. 保留所有权利。”Santa Cruz Biotechnology” 和Santa Cruz Biotechnology, Inc.的商标，”Santa Cruz Animal Health”, “San Juan Ranch”, “Supplement of Champions”, San Juan Ranch的商标，”UltraCruz”, “ChemCruz”, “ImmunoCruz”, “ExactaCruz”, 和 “EZ Touch”是Santa Cruz Biotechnology, Inc.的注册商标。所有商标均为其各自所有者的财产。