Date published: 2025-9-6
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ATF-1/ATF1 Antibody (C41-5.1) - Western Blotting - Image 374018
ATF-1 抗体 (C41-5. 1): sc-243. 在 HeLa (A) 和 F9 (B) 全细胞裂解液以及 NIH/3T3 (C) 和 K-562 (D) 核提取物中 ATF-1 表达的 Western 印迹分析.
ATF-1 抗体 (C41-5. 1): sc-243. 在 HeLa (A) 和 F9 (B) 全细胞裂解液以及 NIH/3T3 (C) 和 K-562 (D) 核提取物中 ATF-1 表达的 Western 印迹分析.

ATF-1/ATF1 抗体 (C41-5.1): sc-243

4.8(10)
寫評論提問

说明书
  • ATF-1/ATF1抗体(C41-5.1)是小鼠单克隆IgA κ, 在104篇文献中引用，规格为200 µg/ml
  • 免疫human物种的重组ATF-1
  • 推荐用于 mouse, rat 和 human 来源的ATF-1 p35 WB 和 IP检测
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-243 X, 200 µg/0.1 ml)
  • 看 ATF-1/ATF1 (25C10G): sc-270，可查看有标记的 ATF-1/ATF1 抗体，包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • 关于如何获取ATF-1/ATF1 (C41-5.1): sc-243的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前，我们还没有完成ATF-1/ATF1 抗体 (C41-5.1)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 ATF 抗体 包括 ATF-1, ATF-2, ATF-3, ATF-5, ATF-6 α, ATF-5 β和ATF-7IP

ATF-1/ATF1抗体（C41-5.1）是一种IgAκ小鼠单克隆ATF-1抗体，可通过WB和IP检测小鼠、大鼠和人类来源的ATF-1 p35。ATF-1/ATF1抗体（C41-5.1）是非偶联的抗ATF-1抗体形式提供。真核基因转录受到序列特异性转录因子结合模块化的cis作用启动子和增强子元件的调控。ATF/CREB转录因子家族结合了回文cAMP应答元件（CRE）八核苷酸序列TGACGTCA。ATF/CREB家族包括CREB-1、CREB-2（也称为ATF-4）、ATF-1、ATF-2和ATF-3。这个蛋白质家族具有高度不同的N末端结构，但共享C末端的亮氨酸拉链以便于二聚体形成和DNA结合。ATF-1被证明在NOX1诱导中起着关键作用。ATF-1结合cAMP应答元件（CRE），介导PKA诱导的CRE报告基因激活。ATF-2形成同源二聚体和与c-Jun的异源二聚体，以启动CRE依赖的转录。应激激活激酶磷酸化ATF-2的Thr 69和Thr 71是转录激活所必需的。Myc也诱导ATF-2在Thr 69和Thr 71的磷酸化，以延长ATF-2的半衰期。ATF-2作为组蛋白乙酰转移酶（HAT），在体外特异性乙酰化组蛋白H2B和H4。

仅限研究使用。不适用于诊断和治疗用途。

订购信息

产品名称产品编号规格价格数量收藏夹

ATF-1/ATF1 抗体 (C41-5.1)

sc-243
200 µg/ml
RMB2377.00

ATF-1/ATF1 抗体 (C41-5.1) X

sc-243 X
200 µg/0.1 ml
RMB2377.00

When should the TransCruz reagent sc-243 X be used instead of the standard form sc-243?

Asked by: jerojero
Thank you for your question. We recommend using the TransCruz reagent form when performing ChIP or GelShift assays. This form can also be used in other applications such as Western Blot or IHC, but the dilution should be adjusted by a factor of 10 to account for the higher original concentration.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from It is fine.Used for WB to detect human cancers. Got the specific band, but too weak. Supersignal ECL is required.
Date published: 2020-08-13
Rated 4 out of 5 by from Specific for ATF1 in WBI overexpressed GFP-tagged ATF1, CREB and CREM in HEK293 cells. ATF1 C41-5.1 was specific to ATF1 in WB, as it recognised only GFP-ATF1 protein, but not GFP-CREB or GFP-CREM. Dilution 1:200 worked very well in WB with overexpressed ATF1 in HEK293 cells, but failed to recognize endogenous ATF1 and gave a very strong background signal in rat primary cortical neuron lysates.
Date published: 2018-08-23
Rated 5 out of 5 by from Great Antibody for WBThis antibody is well-cited for a variety of applications including WB, IP, even ChIP. Strong, clear signal with little to no background. Works well in a variety of species including mouse, rat, and human samples.
Date published: 2017-01-30
Rated 5 out of 5 by from Used this antibody for WB detection and itUsed this antibody for WB detection and it worked well.
Date published: 2015-10-05
Rated 5 out of 5 by from Strong band in WB in HeLa nuclear extractStrong band in WB in HeLa nuclear extract. -SCBT QC
Date published: 2015-04-06
Rated 5 out of 5 by from Data published for WBData published for WB, IP, and EMSA in rat cortical neuron. -SCBT Publication Review
Date published: 2015-03-31
Rated 5 out of 5 by from Published data for WB in mouse RAW264Published data for WB in mouse RAW264.7 and human THP-1 cell lines. -SCBT Publication Review
Date published: 2015-03-27
Rated 5 out of 5 by from Publishable data for EMSA in human JEGPublishable data for EMSA in human JEG-3, JAR, Tera-2, and HeLa cells. -SCBT Publication Review
Date published: 2015-03-17
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ATF-1/ATF1 抗体 (C41-5.1) is rated 4.8 out of 5 by 10.
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