Date published: 2025-9-8

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ATF-1/ATF1 抗体 (25C10G): sc-270

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说明书
  • ATF-1/ATF1 抗体 25C10G 是小鼠单克隆 IgG1 κ,ATF-1/ATF1抗体, 在100篇文献中引用,规格为200 µg/ml
  • 针对氨基酸39-271的抗原,在human的ATF-1的DNA结合和二聚化结构域内
  • ATF-1/ATF1 抗体 (25C10G) 推荐用于 WB, IP, IF 和 IHC(P),检测mouse, rat 和human 来源的 ATF-1 p35, CREB-1 p43 and CREM-1
  • 抗ATF-1/ATF1抗体(25C10G)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-270 X, 200 µg/0.1 ml)
  • 关于如何获取ATF-1/ATF1 (25C10G): sc-270免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRPm-IgG1 BP-HRP 是 ATF-1/ATF1 抗体 (25C10G) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与ATF-1/ATF1 抗体 (25C10G) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

ATF-1抗体(25C10G)是一种小鼠单克隆IgG1 kappa轻链抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)和免疫组织化学(IHC)检测小鼠、大鼠和人源ATF-1。ATF-1 (25C10G) 单克隆抗体有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶、藻红蛋白、异硫氰酸荧光素和多种Alexa Fluor®结合物。ATF-1在调节基因转录方面发挥着关键作用,尤其是在应对细胞应激和信号通路方面。ATF-1是ATF/CREB转录因子家族的一员,该家族对于结合cAMP反应元件(CRE)并促进参与各种生理过程的目标基因的转录至关重要。ATF-1介导NOX1的诱导,凸显了ATF-1在活性氧产生中的重要性,而活性氧的产生对于细胞信号传导和稳态至关重要。ATF-1与其他转录因子(如ATF-2和c-Jun)相互作用,形成复合物,启动CRE依赖性转录,从而影响一系列生物反应。ATF-1在特定残基上的磷酸化对于转录活性至关重要,这凸显了翻译后修饰在调节功能中的重要性。ATF-1 (25C10G)抗体是研究基因调控和ATF-1在各种生物学环境中介导的细胞反应的复杂机制的宝贵工具。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

ATF-1/ATF1 抗体 (25C10G) 参考文献:

  1. ATF-1 在 B16 黑色素瘤细胞对紫外线照射的反应中被激活。  |  Moore, BA., et al. 1999. Mol Cell Biol Res Commun. 1: 1-6. PMID: 10329470
  2. ATF-2 具有固有的组蛋白乙酰转移酶活性,可通过磷酸化进行调节。  |  Kawasaki, H., et al. 2000. Nature. 405: 195-200. PMID: 10821277
  3. 通过单链 Fv 片段靶向人黑色素瘤中的 ATF-1/CREB 转录因子:癌症治疗的潜在模式。  |  Jean, D. and Bar-Eli, M. 2001. Crit Rev Immunol. 21: 275-86. PMID: 11642609
  4. Myc 与激活转录因子 2(ATF2)之间的相互影响:Myc 可延长 ATF2 的半衰期并诱导其磷酸化。  |  Miethe, J., et al. 2001. Oncogene. 20: 8116-24. PMID: 11781825
  5. EGF 受体的反式激活和 PI3 激酶-ATF-1 通路参与了 NADPH 氧化酶催化亚基 NOX1 的上调。  |  Fan, C., et al. 2005. FEBS Lett. 579: 1301-5. PMID: 15710429
  6. 将环磷酸腺苷反应元件结合蛋白 2 (CREB2) 的基因归入人类染色体 2q24.1-q32。  |  Diep, A., et al. 1991. Genomics. 11: 1161-3. PMID: 1838349
  7. 转录因子 ATF cDNA 克隆:能够选择性地形成 DNA 结合异二聚体的大量亮氨酸拉链蛋白家族。  |  Hai, TW., et al. 1989. Genes Dev. 3: 2083-90. PMID: 2516827
  8. 一种常见的细胞转录因子 ATF 与 E1a 和环 AMP 诱导的启动子中的调控元件相互作用。  |  Lin, YS. and Green, MR. 1988. Proc Natl Acad Sci U S A. 85: 3396-400. PMID: 2835770
  9. 鉴定大鼠体生长抑素基因中的环-AMP 反应元件。  |  Montminy, MR., et al. 1986. Proc Natl Acad Sci U S A. 83: 6682-6. PMID: 2875459
  10. MC1R信号通过cAMP-CREB/ATF-1和ERK-NFκB途径加速G1/S转换,促进乳腺癌进展。  |  Chelakkot, VS., et al. 2023. NPJ Precis Oncol. 7: 85. PMID: 37679505
  11. ATF-2 优先被应激活化蛋白激酶激活,在基因毒性物质作用下介导 c-jun 诱导。  |  van Dam, H., et al. 1995. EMBO J. 14: 1798-811. PMID: 7737130
  12. cJun 和 ATF-2 的异源二聚体形成是 243 氨基酸腺病毒 E1A 蛋白诱导 c-jun 的原因。  |  van Dam, H., et al. 1993. EMBO J. 12: 479-87. PMID: 8382609

订购信息

产品名称产品编号规格价格数量收藏夹

ATF-1/ATF1 抗体 (25C10G)

sc-270
200 µg/ml
$316.00

ATF-1/ATF1 (25C10G): m-IgG Fc BP-HRP 套装

sc-526455
200 µg Ab; 10 µg BP
$354.00

ATF-1/ATF1 (25C10G): m-IgG1 BP-HRP 套装

sc-531828
200 µg Ab; 20 µg BP
$354.00

ATF-1/ATF1 抗体 (25C10G) X

sc-270 X
200 µg/0.1 ml
$316.00

ATF-1/ATF1 抗体 (25C10G) AC

sc-270 AC
500 µg/ml, 25% agarose
$416.00

ATF-1/ATF1 抗体 (25C10G) HRP

sc-270 HRP
200 µg/ml
$316.00

ATF-1/ATF1 抗体 (25C10G) FITC

sc-270 FITC
200 µg/ml
$330.00

ATF-1/ATF1 抗体 (25C10G) PE

sc-270 PE
200 µg/ml
$343.00

ATF-1/ATF1 抗体 (25C10G) Alexa Fluor® 488

sc-270 AF488
200 µg/ml
$357.00

ATF-1/ATF1 抗体 (25C10G) Alexa Fluor® 546

sc-270 AF546
200 µg/ml
$357.00

ATF-1/ATF1 抗体 (25C10G) Alexa Fluor® 594

sc-270 AF594
200 µg/ml
$357.00

ATF-1/ATF1 抗体 (25C10G) Alexa Fluor® 647

sc-270 AF647
200 µg/ml
$357.00

ATF-1/ATF1 抗体 (25C10G) Alexa Fluor® 680

sc-270 AF680
200 µg/ml
$357.00

ATF-1/ATF1 抗体 (25C10G) Alexa Fluor® 790

sc-270 AF790
200 µg/ml
$357.00

When should the TransCruz reagent sc-270 X be used instead of the standard form sc-270?

Asked by: DefinitelyNotMatt
Thank you for your question. We recommend using the TransCruz reagent form when performing ChIP or GelShift assays. This form can also be used in other applications such as Western Blot or IHC, but the dilution should be adjusted by a factor of 10 to account for the higher original concentration.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 3 out of 5 by from Recognizes all CREB family members in WBI overexpressed GFP-tagged ATF1, CREB and CREM in HEK293 cells. ATF1 25C10G (dilution 1:200) recognized GFP-ATF1, but was not specific to ATF1 in WB, as it recognised all the GFP-tagged CREB family members.
Date published: 2018-08-23
Rated 5 out of 5 by from Excellent choice for IHC(P) and WBVery well rounded antibody for IHC on paraffin sections with nice specific clean staining. Also great for WB and IF. All around, a great choice!
Date published: 2017-02-01
Rated 5 out of 5 by from improveThe antibody properties are easier to observe, the overall characteristics of dilution preservation intact, than the previous generation has a greater improvement
Date published: 2017-01-17
Rated 5 out of 5 by from good productThe product is good. The result show that the antibody expressed in lung cancer.
Date published: 2016-11-29
Rated 5 out of 5 by from GreatThis production is very great with high quanlity and high specificity.
Date published: 2016-11-19
Rated 5 out of 5 by from Publishable data for WB in human transfected SaosPublishable data for WB in human transfected Saos-2 and U-2 OS cells.
Date published: 2015-04-22
Rated 5 out of 5 by from Good band in WB in KNRK nuclear extractGood band in WB in KNRK nuclear extract.-SCBT QC
Date published: 2015-03-12
Rated 5 out of 5 by from Produced great immunoperoxidase nuclear stainingProduced great immunoperoxidase nuclear staining in formalin fixed, paraffin-embedded human pancreas tissue. -SCBT QC
Date published: 2015-03-03
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ATF-1/ATF1 抗体 (25C10G) is rated 4.8 out of 5 by 11.
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