Date published: 2026-10-24

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ASK 1 抗体 (H-2): sc-390275

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说明书
  • ASK 1抗体(H-2)是小鼠单克隆IgM κ, 在6篇文献中引用,规格为200 µg/ml
  • 特异性抗原位于human物种的ASK 1的N-terminus的氨基酸2-29之间
  • 推荐用于 mouse, rat 和 human 来源的ASK 1 WB, IP, IF 和 ELISA检测
  • 看 ASK 1 (F-9): sc-5294,可查看有标记的 ASK 1 抗体,包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • m-IgGκ BP-HRP是ASK 1 Antibody (H-2) 适用于 WB 应用。 的首选辅助检测试剂。该试剂现可与ASK 1 Antibody (H-2) 搭配使用(请参阅下面的订购信息)。有关其它 m-IgGκ BP 结合物,请参阅我们完整的小鼠 IgG 结合蛋白列表。
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ASK 1抗体(H-2)是一种IgM κ小鼠单克隆ASK 1抗体(也称为ASK 1抗体或MAP3K5抗体),可通过WB、IP、IF和ELISA检测小鼠、大鼠和人类的ASK 1。ASK 1抗体(H-2)以非共轭抗ASK 1抗体的形式存在。丝裂原活化蛋白(MAP)激酶级联由各种细胞外刺激激活,包括生长因子。MEK激酶(也称为MAP激酶激酶激酶、MKKKs、MAP3Ks或MEKKs)通过磷酸化从而激活MEK(也称为MAP激酶激酶或MKK),包括ERK、JNK和p38。这些被激活的MEK反过来磷酸化和激活MAP激酶。MEK激酶包括Raf-1、Raf-B、Mos、MEK激酶-1、MEK激酶-2、MEK激酶-3、MEK激酶-4、ASK 1(MEK激酶-5)和MAP3K6(MEK激酶-6)。已显示MEK激酶-1通过不依赖Raf的途径磷酸化MEK-1。有证据表明,MEK-3优先被MEK激酶-3激活,而MEK-4则被MEK激酶-2和MEK激酶-3共同激活。已显示MEK激酶-4特异性地激活JNK途径。ASK1激活MEK-4和MEK-3/MEK-6两种途径。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

ASK 1 抗体 (H-2) 参考文献:

  1. ASK-1(凋亡信号调节激酶 1)是 E2F 的直接靶基因。  |  Kherrouche, Z., et al. 2006. Biochem J. 396: 547-56. PMID: 16512785
  2. RNA干扰细胞凋亡信号调节激酶-1(ASK-1)可挽救rd1小鼠的感光细胞死亡。  |  Sekimukai, D., et al. 2009. Mol Vis. 15: 1764-73. PMID: 19727340
  3. SCO2 通过 ASK-1 的 Thr845 磷酸化和 ASK-1-Trx 复合物的解离诱导 p53 介导的细胞凋亡。  |  Madan, E., et al. 2013. Mol Cell Biol. 33: 1285-302. PMID: 23319048
  4. SOCS-1 通过 ASK-1 挽救了 IL-1β 介导的对小鼠肺上皮细胞上皮钠通道的抑制。  |  Galam, L., et al. 2016. Oncotarget. 7: 29081-91. PMID: 27058411
  5. ASK家族的调节和信号传导机制。  |  Nishida, T., et al. 2017. Adv Biol Regul. 66: 2-22. PMID: 28669716
  6. 羟基茚酮衍生物TI-1-162通过抑制RIP/ASK-1/MAPK信号传导,对TNBS诱导的大鼠结肠炎具有改善作用。  |  Gurung, P., et al. 2018. Eur J Pharmacol. 827: 94-102. PMID: 29555502
  7. 在生殖细胞凋亡过程中,硫氧还蛋白系统受ASK-1/JNK/p38/Survivin途径调节。  |  Al-Kandari, N., et al. 2019. Molecules. 24: PMID: 31547465
  8. N-乙酰半胱氨酸对暴露于镉的雄性大鼠肝脏中c-myc和Ask-1基因表达、组织病理学、氧化应激、炎症和凋亡的影响。  |  Alizadeh, B., et al. 2022. Biol Trace Elem Res. 200: 661-668. PMID: 33740178
  9. 己酮可可碱治疗减轻了肾脏缺血/再灌注损伤:甘露聚糖-3和ASK-1/JNK和ERK1/2/NF-κB/HMGB-1轨迹的新参与。  |  El-Sadek, HM., et al. 2021. J Pharmacol Sci. 146: 136-148. PMID: 34030796
  10. 芝麻素通过抑制ASK-1-JNK/p38 MAPK通路减轻紫外线诱导的角朊细胞损伤  |  Li, H., et al. 2024. J Cosmet Dermatol. 23: 316-325. PMID: 37545137

订购信息

产品名称产品编号规格价格数量收藏夹

ASK 1 抗体 (H-2)

sc-390275
200 µg/ml
CNY2422.00

ASK 1 (H-2): m-IgGκ BP-HRP 套装

sc-552510
200 µg Ab; 40 µg BP
CNY2715.00

ASK 1 (H-2) 中和勝肽

sc-390275 P
100 µg/0.5 ml
CNY519.00

What application is the blocking peptide sc-390275 P appropriate for?

Asked by: Dr Ninau Qelp
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by jerojero from Excellent!This antibody works great in western blot analysis of ASK 1 expression in NIH/3T3 whole cell lysate.
Date published: 2017-02-06
Rated 5 out of 5 by Like hao from Good WB data with human cellGreat Western Blot data with human cell ,diluted with 1:500,but it was not well for IP
Date published: 2017-01-10
Rated 5 out of 5 by JL from Produced great immunofluorescence cytoplasmicProduced great immunofluorescence cytoplasmic staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-03-20
Rated 5 out of 5 by MS from Great Western Blot data of ASK 1 expression in nonGreat Western Blot data of ASK 1 expression in non-transfected and human ASK 1 transfected 293T whole cell lysates. -SCBT QC
Date published: 2013-04-22
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ASK 1 抗体 (H-2) is rated 5.0 out of 5 by 4.
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