Date published: 2025-9-8
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ASCL1 Antibody (D-7) - Western Blotting - Image 301360
ASCL1 抗体 (D-7): sc-374104. 对 SHP-77 全细胞裂解液中 ASCL1 的表达进行 Western 印迹分析.
ASCL1 Antibody (D-7) - Western Blotting - Image 301360
ASCL1 抗体 (D-7): sc-374104. 对 SHP-77 全细胞裂解液中 ASCL1 的表达进行 Western 印迹分析.
ASCL1 Antibody (D-7) - Western Blotting - Image 148738
ASCL1 抗体 (D-7): sc-374104. 非转染: sc-117752 (A) 和人 ASCL1 转染: sc-171675 (B) 293T 全细胞裂解液中 ASCL1 表达的 Western 印迹分析.
ASCL1 抗体 (D-7): sc-374104. 对 SHP-77 全细胞裂解液中 ASCL1 的表达进行 Western 印迹分析.

ASCL1 抗体 (D-7): sc-374104

4.0(9)
寫評論提問

说明书
  • ASCL1抗体(D-7)是小鼠单克隆IgG2a κ, 在18篇文献中引用，规格为200 µg/ml
  • 免疫原氨基酸序列181-236位于human物种的ASCL1的C-terminus
  • 抗-ASCL1 抗体 (D-7) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 ASCL1; 也和以下物种反应，包括： canine
  • 抗ASCL1抗体（D-7）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于ChIP的TransCruz试剂(sc-374104 X, 200 µg/0.1 ml)
  • 关于如何获取ASCL1 (D-7): sc-374104的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP、 2a BP-HRP">m-IgG2a BP-HRP和m-IgGκ BP-HRP是ASCL1 Antibody (D-7) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与ASCL1 Antibody (D-7) 打包提供（请参阅下面的订购信息）。

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相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

附加Achaete-Scute抗体，包括Achaete Scute 1

ASCL1抗体（D-7）是一种IgG2a κ小鼠单克隆ASCL1抗体，可通过蛋白质印迹、免疫沉淀、免疫荧光和酶联免疫吸附试验检测小鼠、大鼠和人的ASCL1。ASCL1抗体（D-7）既可以作为非偶联的抗ASCL1抗体形式提供，也可以作为多种偶联形式的抗ASCL1抗体提供，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。果蝇蛋白achaete-scute的哺乳动物同源物ASCL1（也称为ASH1）是一种碱性螺旋-环-螺旋转录因子，对神经系统的早期发育至关重要。ASCL1在胎儿大脑中表达，对于自主神经元的正常发育和自主神经元亚群的存活至关重要。ASCL1与MEF-2A的相互作用可能调节特定基因的表达，这些基因对于中枢神经系统内不同神经元回路的形成至关重要。ASCL1在神经内分泌肿瘤中的高水平表达，如甲状腺髓样癌、小细胞肺癌和具有神经内分泌特征的肺癌，可能为具有神经内分泌特征的癌症提供有用的标记物。ASCL1基因定位于人类12号染色体，包含一个三核苷酸重复区域，使该位点成为遗传性疾病的候选基因。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

ASCL1 抗体 (D-7)

sc-374104
200 µg/ml
RMB2377.00

ASCL1 (D-7): m-IgG Fc BP-HRP 套装

sc-529661
200 µg Ab; 10 µg BP
RMB2662.00

ASCL1 (D-7): m-IgGκ BP-HRP 套装

sc-522623
200 µg Ab, 40 µg BP
RMB2662.00

ASCL1 (D-7): m-IgG2a BP-HRP 套装

sc-547353
200 µg Ab; 10 µg BP
RMB2662.00

ASCL1 抗体 (D-7) X

sc-374104 X
200 µg/0.1 ml
RMB2377.00

ASCL1 抗体 (D-7) AC

sc-374104 AC
500 µg/ml, 25% agarose
RMB3129.00

ASCL1 抗体 (D-7) HRP

sc-374104 HRP
200 µg/ml
RMB2377.00

ASCL1 抗体 (D-7) FITC

sc-374104 FITC
200 µg/ml
RMB2482.00

ASCL1 抗体 (D-7) PE

sc-374104 PE
200 µg/ml
RMB2580.00

ASCL1 抗体 (D-7) Alexa Fluor® 488

sc-374104 AF488
200 µg/ml
RMB2685.00

ASCL1 抗体 (D-7) Alexa Fluor® 546

sc-374104 AF546
200 µg/ml
RMB2685.00

ASCL1 抗体 (D-7) Alexa Fluor® 594

sc-374104 AF594
200 µg/ml
RMB2685.00

ASCL1 抗体 (D-7) Alexa Fluor® 647

sc-374104 AF647
200 µg/ml
RMB2685.00

ASCL1 抗体 (D-7) Alexa Fluor® 680

sc-374104 AF680
200 µg/ml
RMB2685.00

ASCL1 抗体 (D-7) Alexa Fluor® 790

sc-374104 AF790
200 µg/ml
RMB2685.00

What's the antigen region of the antibody? targeted to the C-terminal? Thanks!

Asked by: Quentin
Thank you for your question. The epitope range is 181-236. https://www.uniprot.org/uniprotkb/Q02067/entry
Answered by: BlakeJ
Date published: 2023-09-19

I would like to test this antibody for IHC on fixed-flash frozen tissue specimens. Do you offer trial aliquot samples? I can provide you with the results of this test, including pictures.

Asked by: Aldrin Molero
Please contact our Technical Service Department for more information on how to receive a free 10 ug sample of this monoclonal antibody.
Answered by: Technical Support
Date published: 2019-03-01

What is the recommended starting dilution for Western blot for sc-374104: ASCL1 (D-7) monoclonal antibody?

Asked by: Dr Ninau Qelp
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-22
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Rated 1 out of 5 by from no bandsASCL1 (D-7) – sc-374104-sample We failed to detect ASCL1 at tested concentrations (up to 1:100) in MCF7, BT549, or U87.
Date published: 2025-06-16
Rated 3 out of 5 by from Funziona bene su proteina overespressaTestato questo anticorpo su cellule MCF-7. Preparato 1:500 in latte 5% con incubazione o.n. Funziona bene solo su cellule che esprimono parecchia proteina.
Date published: 2025-03-19
Rated 5 out of 5 by from Works great for WBWorks great for WB, clean, strong band. Only expressed in expected cell lines but not cells that shouldn't express it.
Date published: 2018-07-15
Rated 4 out of 5 by from Antibody reviewThis antibody works at 1:500 dilution for immunoblotting in mouse tissue lysate.
Date published: 2018-06-14
Rated 4 out of 5 by from Good but high backgroundUsed for immunohistochemistry on embryonic spinal cord cryo-sections at a concentration of 1:100. Good staining but high background.
Date published: 2018-02-16
Rated 4 out of 5 by from Nice resultI used thsi antibody for IHC on FFPE tissue and it produced a nice result.
Date published: 2017-12-13
Rated 5 out of 5 by from Clean resultsGood detection seen of endogenous and transfected ASCL1 in Western Blot with no background or additional bands.
Date published: 2017-02-02
Rated 5 out of 5 by from Nice Western Blot data of ASCL1 expression in SHPNice Western Blot data of ASCL1 expression in SHP-77 whole cell lysate. -SCBT QC
Date published: 2015-03-17
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ASCL1 抗体 (D-7) is rated 4.0 out of 5 by 9.
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