Date published: 2025-9-8
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ARK-1 Antibody (C-1) - Western Blotting - Image 374730
ARK-1 抗体 (C-1): sc-398814. HeLa (A), Caco-2 (B), TF-1 (C) 和 A-431 (D) 全细胞裂解液中 ARK-1 表达的 Western 印迹分析.
ARK-1 抗体 (C-1): sc-398814. HeLa (A), Caco-2 (B), TF-1 (C) 和 A-431 (D) 全细胞裂解液中 ARK-1 表达的 Western 印迹分析.

ARK-1 抗体 (C-1): sc-398814

4.6(7)
寫評論提問

说明书
  • ARK-1抗体(C-1)是小鼠单克隆IgG1 κ, 在1篇文献中引用，规格为200 µg/ml
  • 免疫原氨基酸序列1-130位于human物种的ARK-1的N-terminus
  • ARK-1 抗体 (C-1) 推荐用于WB, IP, IF, IHC(P) 和 ELISA，检测human 来源的 ARK-1
  • 抗ARK-1抗体（C-1）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取ARK-1 (C-1): sc-398814的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 ARK-1 抗体 (C-1) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与ARK-1 抗体 (C-1) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 ARK 抗体 包括 ARK-1, ARK-2和NUAK1/ARK5

ARK-1抗体（C-1）是一种IgG1 κ小鼠单克隆ARK-1抗体，可通过WB、IP、IF、IHC(P)和ELISA检测人源ARK-1。ARK-1抗体（C-1）既可以是非偶联的抗ARK-1抗体形式，也可以是多种偶联形式的抗ARK-1抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。极光相关激酶-1（ARK-1、STK15、Aurora2、Aik1）和-2（ARK-2、STK12、Aurora1）是与中心体相关的丝氨酸/苏氨酸激酶，它们在有丝分裂过程中调节中心体分离、双极纺锤体组装和染色体分离。ARK-1和ARK-2在细胞核中表达，并在有丝分裂期间定位于有丝分裂机械的不同部分，如中心体、纺锤极（ARK-1）和中体（ARK-2）。ARK-1和ARK-2的转录本在人胸腺和胎儿肝脏中高水平存在。ARK-1蛋白在结肠癌细胞系（HT-29、SNU-C2B、COLO 205、SW480、837和948）中表达升高，并在HeLa细胞的中期累积。ARK-2蛋白水平在S期和G2/M期均达到最大，而ARK-1蛋白在G2/M期后通过泛素-蛋白酶体途径降解。ARK-2相对于ARK-1具有独特的基因位点，表明这两种具有致癌潜力的激酶在细胞周期进程中具有不同的作用。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

ARK-1 抗体 (C-1) 参考文献:

  1. 通过基于配体的层次化虚拟筛选鉴定新型极光激酶 A (AURKA) 抑制剂。  |  Kong, Y., et al. 2018. J Chem Inf Model. 58: 36-47. PMID: 29202231
  2. 极光激酶A（AURKA）会促进晚期胃肠道间质瘤的进展和伊马替尼的耐药性。  |  Cheng, X., et al. 2021. Cancer Cell Int. 21: 407. PMID: 34332577
  3. 极光激酶 A 通过巨噬细胞极化和 Wnt/β-catenin 信号调节肝脏再生。  |  Yin, Y., et al. 2022. Liver Int. 42: 468-478. PMID: 34719108
  4. 作为恶性间皮瘤诊断和预后标志物的极光激酶 A  |  Guo, Z., et al. 2021. Front Oncol. 11: 789244. PMID: 34956905
  5. 针对癌症中的极光激酶 a (AURKA)：潜在 AURKA 抑制剂的分子对接和动态模拟。  |  Almilaibary, A. 2022. Med Oncol. 39: 246. PMID: 36180808
  6. AURKA/DDX5/TMEM147-AS1/let-7的反馈回路驱动噬脂作用，诱导上皮性卵巢癌的顺铂耐药性。  |  Shao, Y., et al. 2023. Cancer Lett. 565: 216241. PMID: 37217070
  7. 极光激酶 A 调节乳腺癌中癌症相关 RNA 的异常剪接。  |  Li, S., et al. 2023. Heliyon. 9: e17386. PMID: 37415951
  8. eEF1A2 可促进 PTEN-GSK3β-SCF 复合物对极光激酶 A 的依赖性降解，并在乳腺癌中失活。  |  Treekitkarnmongkol, W., et al. 2024. Sci Signal. 17: eadh4475. PMID: 38442201
  9. TonEBP 通过控制极光激酶 A 和调节中心极卫星的完整性来抑制纤毛的生成。  |  Chinbold, B., et al. 2024. Cell Commun Signal. 22: 348. PMID: 38961488
  10. Caspase介导的AURKA在Asp132处的裂解对紫杉醇诱导细胞凋亡至关重要。  |  Chen, X., et al. 2024. Theranostics. 14: 3909-3926. PMID: 38994036

订购信息

产品名称产品编号规格价格数量收藏夹

ARK-1 抗体 (C-1)

sc-398814
200 µg/ml
RMB2377.00

ARK-1 (C-1): m-IgG Fc BP-HRP 套装

sc-530939
200 µg Ab; 10 µg BP
RMB2662.00

ARK-1 (C-1): m-IgGκ BP-HRP 套装

sc-524499
200 µg Ab, 40 µg BP
RMB2662.00

ARK-1 (C-1): m-IgG1 BP-HRP 套装

sc-544326
200 µg Ab; 20 µg BP
RMB2662.00

ARK-1 抗体 (C-1) AC

sc-398814 AC
500 µg/ml, 25% agarose
RMB3129.00

ARK-1 抗体 (C-1) HRP

sc-398814 HRP
200 µg/ml
RMB2377.00

ARK-1 抗体 (C-1) FITC

sc-398814 FITC
200 µg/ml
RMB2482.00

ARK-1 抗体 (C-1) PE

sc-398814 PE
200 µg/ml
RMB2580.00

ARK-1 抗体 (C-1) Alexa Fluor® 488

sc-398814 AF488
200 µg/ml
RMB2685.00

ARK-1 抗体 (C-1) Alexa Fluor® 546

sc-398814 AF546
200 µg/ml
RMB2685.00

ARK-1 抗体 (C-1) Alexa Fluor® 594

sc-398814 AF594
200 µg/ml
RMB2685.00

ARK-1 抗体 (C-1) Alexa Fluor® 647

sc-398814 AF647
200 µg/ml
RMB2685.00

ARK-1 抗体 (C-1) Alexa Fluor® 680

sc-398814 AF680
200 µg/ml
RMB2685.00

ARK-1 抗体 (C-1) Alexa Fluor® 790

sc-398814 AF790
200 µg/ml
RMB2685.00

What is the recommended starting dilution for Western blot for sc-398814: ARK-1 (C-1) monoclonal antibody?

Asked by: Randy McDonald
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-22
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Rated 4 out of 5 by from GoodARK-1 (C-1): sc-398814 The antibody was tested on whole lysate from liver cancer cell lines (JHH6 andHuh7) and immortalized hepatocytes (IHH). Antibody: show various bands which can represent shorter or longer forms of the protein. Apparent MW: about 22kDa, 46kDa, and 55kDa. Expected MW: 46kDa. • 40ug/lane cell lysate • Transfer 1h30 100V, PVDF (0.2 µm) • Blocking milk 4% in 0.1% Tween -PBS 1h RT • Anti- ARK-1 (C-1) (sc-398814):200 O/N 4°C in blocking solution • Anti-mouse HRP 1:1000 1h RT in blocking solution • Developed with ECL Plus
Date published: 2022-06-09
Rated 5 out of 5 by from Works very well!We bought this antibody a month ago. It is highly sensitive and shows no background.
Date published: 2018-07-27
Rated 5 out of 5 by from Works great!I used this antibody in a Western blot to validate an AURKA siRNA treatment with good results. It gives a clear band at ~50kD at 1:1000 dilution. There is also a nonspecific band at ~25kD.
Date published: 2017-09-13
Rated 5 out of 5 by from very good for ARK-1Very strong band was shown at a dilution of 1:800 in HepG2 cells.
Date published: 2017-07-06
Rated 5 out of 5 by from ImmunefluorescenceVery good results in IF staining of human adrenal and fibroblasts cells at a concentration of 1:50.
Date published: 2017-06-14
Rated 3 out of 5 by from Reasonably potent and fairly specific antibodyThis monoclonal antibody was independently tested in-house by Kinexus Bioinformatics Corporation and validated for use for Western blotting. There was detection of the ~46 kDa target in a HeLa/Jurkat cell lysate mix, but not in mouse tissues (brain/testes/thymus/muscle mix). No major cross-reactivities were observed at 2 µg/ml antibody concentration with off-target proteins in the mouse tissues tested, except for ~52,~ 34 and ~20 kDa proteins. A ~20 kDa medium cross-reactive protein was also evident in the HeLa/Jurkat cell lysate mix.
Date published: 2016-11-10
Rated 5 out of 5 by from Great Western blot data of ARKGreat Western blot data of ARK-1 expression in non-transfected 293 human ARK-1 transfected 293 HeLa and BT-20 whole cell lysates. -SCBT QC
Date published: 2014-01-05
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ARK-1 抗体 (C-1) is rated 4.6 out of 5 by 7.
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