Date published: 2025-9-7
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ARID2 Antibody (E-3) - Western Blotting - Image 137742
ARID2 抗体 (E-3): sc-166117. 未转染: sc-117752 (A) 和转染人 BAF200: sc-175552 (B) 293T 全细胞裂解液及 HeLa (C), Jurkat (D) 和 MCF7 (E) 核提取物中 ARID2 表达的 Western 印迹分析.
ARID2 抗体 (E-3): sc-166117. 未转染: sc-117752 (A) 和转染人 BAF200: sc-175552 (B) 293T 全细胞裂解液及 HeLa (C), Jurkat (D) 和 MCF7 (E) 核提取物中 ARID2 表达的 Western 印迹分析.

ARID2 抗体 (E-3): sc-166117

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说明书
  • ARID2 抗体 E-3 是小鼠单克隆 IgG1 κ，ARID2抗体, 在27篇文献中引用，规格为200 µg/ml
  • 针对氨基酸1134-1316的图谱，在ARID2的内部区域，human起源
  • ARID2 抗体 (E-3) 推荐用于WB, IP, IF, IHC(P) 和 ELISA，检测human 来源的 ARID2
  • 抗ARID2抗体（E-3）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-166117 X, 200 µg/0.1 ml)
  • 关于如何获取ARID2 (E-3): sc-166117的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 ARID2 抗体 (E-3) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与ARID2 抗体 (E-3) 组合成套装提供。 (请参阅下面的订购信息)。

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相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

此外 ARID 抗体 包括 ARID1B, ARID2, ARID3A和ARID3B

ARID2抗体(E-3)是一种IgG1 κ型小鼠单克隆ARID2抗体(也称为ARID2抗体)，可通过WB、IP、IF、IHC(P)和ELISA检测人源ARID2蛋白。ARID2抗体(E-3)既可以是非偶联的抗ARID2抗体形式，也可以是多种偶联的抗ARID2抗体形式，包括琼脂糖，HRP, PE, FITC和多个Alexa Fluor®偶联物。BAF200 (at丰富的相互作用域蛋白2,brg1相关因子200)是由人类基因ARID2编码的1 835个氨基酸组成的蛋白。BAF200是一种核蛋白，属于染色质重塑复合体SWI/SNF家族，含有一个ARID结构域。通过染色质重塑(dna -核小体拓扑结构的改变)参与选定基因的转录激活和抑制。BAF200对BAF (SWI/SNF-A)和PBAF (SWI/SNF-B)染色质重塑复合物的稳定性是必需的。它也可能参与靶向SWI/SNF复合体到不同的基因。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

ARID2 抗体 (E-3) 参考文献:

  1. ATP 依赖性染色质重塑机制  |  Whitehouse, I., et al. 2000. Biochem Soc Trans. 28: 376-9. PMID: 10961923
  2. 了解 Swi/Snf 复合物重塑染色质的最新进展。  |  Martens, JA. and Winston, F. 2003. Curr Opin Genet Dev. 13: 136-42. PMID: 12672490
  3. 美国国立卫生研究院全长 cDNA 项目：哺乳动物基因库 (MGC) 的现状, 质量和扩展。  |  Gerhard, DS., et al. 2004. Genome Res. 14: 2121-7. PMID: 15489334
  4. ARID 家族蛋白的 DNA 结合特性。  |  Patsialou, A., et al. 2005. Nucleic Acids Res. 33: 66-80. PMID: 15640446
  5. DNA 结合蛋白 ARID 家族的命名法。  |  Wilsker, D., et al. 2005. Genomics. 86: 242-51. PMID: 15922553
  6. PBAF 染色质重塑复合物需要一种新型特异性亚基 BAF200 来调节选择性干扰素应答基因的表达。  |  Yan, Z., et al. 2005. Genes Dev. 19: 1662-7. PMID: 15985610
  7. Zipzap/p200是一种有助于心脏基因调控的新型锌指蛋白。  |  Zhang, X., et al. 2006. Biochem Biophys Res Commun. 346: 794-801. PMID: 16782067
  8. 长非编码 RNA Arid2-IR 通过与 Smad3 结合影响高级糖化终产物诱导的人视网膜内皮细胞损伤。  |  Xiao, H., et al. 2020. Int Ophthalmol. 40: 1123-1133. PMID: 31912402
  9. ARID2 是多发性骨髓瘤细胞中依赖于泊马度胺的 CRL4CRBN 底物。  |  Yamamoto, J., et al. 2020. Nat Chem Biol. 16: 1208-1217. PMID: 32958952
  10. ARID1A、ARID1B和ARID2突变是非小细胞肺癌患者接受免疫检查点阻断治疗的潜在生物标记物  |  Zhu, G., et al. 2021. Front Immunol. 12: 670040. PMID: 34512623
  11. ARID2抑制可促进肿瘤进展，并上调TP53突变型烟草相关口腔癌中的细胞角蛋白8、18和β-4整合素表达，具有预后意义。  |  Shukla, P., et al. 2022. Cancer Gene Ther. 29: 1908-1917. PMID: 35869277
  12. Arid2-IR 通过甲基化下调 miR-132-3p，促进 LPS 诱导的肺炎 ALI。  |  Liu, Y., et al. 2022. Inhal Toxicol. 34: 297-303. PMID: 36074605

订购信息

产品名称产品编号规格价格数量收藏夹

ARID2 抗体 (E-3)

sc-166117
200 µg/ml
RMB2377.00

ARID2 (E-3): m-IgG Fc BP-HRP 套装

sc-528908
200 µg Ab; 10 µg BP
RMB2662.00

ARID2 (E-3): m-IgGκ BP-HRP 套装

sc-521512
200 µg Ab, 40 µg BP
RMB2662.00

ARID2 (E-3): m-IgG1 BP-HRP 套装

sc-543203
200 µg Ab; 20 µg BP
RMB2662.00

ARID2 抗体 (E-3) X

sc-166117 X
200 µg/0.1 ml
RMB2377.00

ARID2 抗体 (E-3) AC

sc-166117 AC
500 µg/ml, 25% agarose
RMB3129.00

ARID2 抗体 (E-3) HRP

sc-166117 HRP
200 µg/ml
RMB2377.00

ARID2 抗体 (E-3) FITC

sc-166117 FITC
200 µg/ml
RMB2482.00

ARID2 抗体 (E-3) PE

sc-166117 PE
200 µg/ml
RMB2580.00

ARID2 抗体 (E-3) Alexa Fluor® 488

sc-166117 AF488
200 µg/ml
RMB2685.00

ARID2 抗体 (E-3) Alexa Fluor® 546

sc-166117 AF546
200 µg/ml
RMB2685.00

ARID2 抗体 (E-3) Alexa Fluor® 594

sc-166117 AF594
200 µg/ml
RMB2685.00

ARID2 抗体 (E-3) Alexa Fluor® 647

sc-166117 AF647
200 µg/ml
RMB2685.00

ARID2 抗体 (E-3) Alexa Fluor® 680

sc-166117 AF680
200 µg/ml
RMB2685.00

ARID2 抗体 (E-3) Alexa Fluor® 790

sc-166117 AF790
200 µg/ml
RMB2685.00

Buenas tardes. Me gustaría saber cómo podríamos pedir una muestra de este anticuerpo de ARID2 (E-3) para poder testarlo antes de comprarlo. Muchas gracias. Un saludo.

Asked by: PPFe
Muchas gracias por su pregunta. Contacte con nuestro servicio tecnico para recibir GRATIS 10 µg de muestra de ARID2 (E-3): sc-166117
Answered by: Technical Support Europe
Date published: 2019-02-04

I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using with c-Src (H-12): sc-5266?

Asked by: TinTin
We recommend using one of our our exclusive Mouse IgG Binding Proteins (m-IgGκ) conjugated to CFL 680 or CFL 790. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 5
Date published: 2017-01-11

Is this ARID2 Antibody sc-166117 available directly conjugated to HRP or fluorescent dyes?

Asked by: TinTin
Thank you for your question. Yes, this antibody is available conjugated to agarose (sc-166117 AC), HRP (sc-166117 HRP), phycoerythrin (sc-166117 PE), fluorescein (sc-166117 FITC), Alexa Fluor® 488 (sc-166117 AF488) and Alexa Fluor® 647 (sc-166117 AF647).
Answered by: Technical Support 5
Date published: 2017-01-11
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Rated 5 out of 5 by from Great for WBThis antibody is great for WB. We confirmed the specificity of the antibody by running an ARID2 KO cell line and no band was detected in the KO sample.
Date published: 2024-04-16
Rated 3 out of 5 by from Spotty performanceReceived a sample and tried it but didn't work very well. Control samples sometimes resulted in a band and sometimes didn't.
Date published: 2017-05-18
Rated 5 out of 5 by from Superb !!Great product for multiple applications including WB,IF and IHC
Date published: 2017-01-29
Rated 5 out of 5 by from Great IF dataThe ARID2 Antibody (E-3): sc-166117 provides excellent staining pattern for immunfluorescence with nuclear localization.
Date published: 2017-01-13
Rated 5 out of 5 by from very good for IFvery good for IF
Date published: 2015-10-15
Rated 5 out of 5 by from Strong band in WB in human transfected 293TStrong band in WB in human transfected 293T whole cell lysates and HeLa, Jurkat and MCF7 nuclear extracts. -SCBT QC
Date published: 2015-06-06
Rated 5 out of 5 by from Produced beautiful immunofluorescence nuclearProduced beautiful immunofluorescence nuclear staining in formalin-fixed HepG2 cells. -SCBT QC
Date published: 2015-04-18
Rated 5 out of 5 by from Robust signal in WB in HEK293 whole cell lysateRobust signal in WB in HEK293 whole cell lysate. -SCBT QC
Date published: 2015-04-15
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ARID2 抗体 (E-3) is rated 4.8 out of 5 by 9.
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