Date published: 2025-9-7
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Arginase 2/ARG2 Antibody (A-10) - Immunohistochemistry - Image 334003
Arg2 抗体 (A-10): sc-393496. 福尔马林固定, 石蜡包埋的人前列腺组织的免疫过氧化物酶染色显示腺细胞的胞浆染色.
Arg2 抗体 (A-10): sc-393496. 福尔马林固定, 石蜡包埋的人前列腺组织的免疫过氧化物酶染色显示腺细胞的胞浆染色.

Arginase 2/ARG2 抗体 (A-10): sc-393496

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说明书
  • Arginase 2/ARG2 抗体 A-10 是小鼠单克隆 IgA κ，Arginase 2/ARG2抗体, 在11篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于human物种的arginase II的C-terminus附近的氨基酸304-335之间
  • 推荐用于 mouse, rat 和 human 来源的Arg2 WB, IP, IF, IHC(P) 和 ELISA检测; 也和以下物种反应，包括： 和 canine, bovine and porcine
  • 可提供偶联琼脂糖 的产品，应用于IP；偶联藻红蛋白,FITC, Alexa Fluor® 488，或者Alexa Fluor® 647的产品, 应用于IF, IHC(P)和FCM
  • 关于如何获取Arginase 2/ARG2 (A-10): sc-393496的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前，我们还没有完成Arginase 2/ARG2 抗体 (A-10)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 Arginase 抗体 包括 Arg2

Arg2抗体（A-10）是一种IgA κ小鼠单克隆Arg2抗体（也称为精氨酸酶2抗体或精氨酸酶II抗体），可通过WB、IP、IF、IHC(P)和ELISA方法检测到小鼠、大鼠和人源的Arg2蛋白。Arg2抗体（A-10）既可以是非偶联的抗Arg2抗体形式，也可以是多种偶联形式的抗精氨酸酶II抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。精氨酸酶1（也称为肝型精氨酸酶）几乎只在肝脏中表达，它催化精氨酸转化为鸟氨酸和尿素。人类精氨酸酶1基因定位于6号染色体q23区，编码一个由322个氨基酸组成的蛋白质。精氨酸酶1以同源三聚体蛋白的形式存在，并含有一个双核锰簇。Arg2催化与精氨酸酶1相同的反应，但在组织特异性和亚细胞定位上有所不同。具体而言，精氨酸酶II定位于线粒体。Arg2在非肝组织中表达，在肾脏中的表达水平最高，但与精氨酸酶1不同的是，它不在肝脏中表达。人类Arg2基因定位于14号染色体q24.1-q24.3区，编码一个由354个氨基酸组成的蛋白质。此外，Arg2还包含一个推测的氨基端线粒体定位序列。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Arginase 2/ARG2 抗体 (A-10) 参考文献:

  1. 人类精氨酸酶四元结构中 S 形结构域的含义。  |  Mora, A., et al. 2000. Biochim Biophys Acta. 1476: 181-90. PMID: 10669784
  2. 精氨酸酶的结构和功能。  |  Ash, DE. 2004. J Nutr. 134: 2760S-2764S; discussion 2765S-2767S. PMID: 15465781
  3. 通过饮食中缺锰来降低精氨酸酶的活性，可增强大鼠主动脉内皮依赖性血管舒张。  |  Ensunsa, JL., et al. 2004. Exp Biol Med (Maywood). 229: 1143-53. PMID: 15564441
  4. 监测肝移植后肝细胞功能的精氨酸酶活性浓度标记--初步报告。  |  Grodzicki, M., et al. 2004. Ann Transplant. 9: 54-7. PMID: 15759549
  5. 经肝动脉栓塞引起的内源性肝脏精氨酸酶全身释放诱导的精氨酸耗竭性肝细胞癌缓解，大剂量胰岛素增强了这种缓解：精氨酸酶是治疗肝细胞癌的潜在候选药物。  |  Cheng, PN., et al. 2005. Cancer Lett. 224: 67-80. PMID: 15911102
  6. ETNPPL通过调节ARG2-ROS信号轴损害自噬，导致棕榈酸诱导的肝胰岛素抵抗。  |  Wang, C., et al. 2023. Free Radic Biol Med. 199: 126-140. PMID: 36841363
  7. ARG2敲除通过调节NF-κB和Wnt/β-catenin信号级联，促进腺肌症细胞G0/G1期停滞和线粒体功能障碍。  |  Xu, Y., et al. 2024. Int Immunopharmacol. 140: 112817. PMID: 39116499
  8. 肝脏精氨酸酶两种不同分子质量组成亚基的免疫学特性。  |  Diez, A., et al. 1994. Biol Chem Hoppe Seyler. 375: 537-41. PMID: 7811393
  9. 非肝脏线粒体精氨酸酶（精氨酸酶 II）cDNA 的分子克隆及其在小鼠巨噬细胞样细胞系中与一氧化氮合酶诱导作用的比较。  |  Gotoh, T., et al. 1996. FEBS Lett. 395: 119-22. PMID: 8898077
  10. 人类精氨酸酶 II 基因的染色体定位及其 mRNA 的组织分布。  |  Gotoh, T., et al. 1997. Biochem Biophys Res Commun. 233: 487-91. PMID: 9144563
  11. 败血症患者肺部精氨酸酶和 iNOS 的差异表达。  |  Carraway, MS., et al. 1998. Exp Lung Res. 24: 253-68. PMID: 9635249
  12. 肝脏精氨酸酶缺乏导致的高精氨酸血症。  |  Crombez, EA. and Cederbaum, SD. 2005. Mol Genet Metab. 84: 243-51. PMID: 15694174

订购信息

产品名称产品编号规格价格数量收藏夹

Arginase 2/ARG2 抗体 (A-10)

sc-393496
200 µg/ml
RMB2377.00

Arg2 抗体 (A-10) AC

sc-393496 AC
500 µg/ml, 25% agarose
RMB3129.00

Arg2 抗体 (A-10) Alexa Fluor® 488

sc-393496 AF488
200 µg/ml
RMB2685.00

Arg2 抗体 (A-10) Alexa Fluor® 647

sc-393496 AF647
200 µg/ml
RMB2685.00

Arg2 抗体 (A-10) FITC

sc-393496 FITC
200 µg/ml
RMB2482.00

Arg2 抗体 (A-10) HRP

sc-393496 HRP
200 µg/ml
RMB2377.00

Arg2 (A-10) 中和勝肽

sc-393496 P
100 µg/0.5 ml
RMB511.00

Arg2 抗体 (A-10) PE

sc-393496 PE
200 µg/ml
RMB2580.00

Why does the image in the datasheet show a band size of 90kDa when the expected protein size is 40kDa. This antibody is suggested as a replacement for H-64 which was a rabbit monoclonal at 40kDa. So does this not detect the same protein? Thanks.

Asked by: stonology
Thank you for your question. According to the literature and our data in arginase II transfected lysates, the expected molecular weight for arginase II is approximately 40 kda. Unfortunately, we do not have any reference to support a molecular weight of 90 kda in cell lines at this time. Therefore, we are going to remove that data from our website and reevaluate the antibody in different cell lines. This mouse monoclonal antibody, arginase II (A-10): sc-393496, does detect the same arginase II protein as the now discontinued rabbit polyclonal antibody.
Answered by: Technical Support
Date published: 2017-12-07

I have two questions. First, in your data sheet, molecular weights is 40kDa. But figure of WB band says 90kDa. Is it fusion band? I wonder real band size.  Next, what secondary antibody should I use for western blotting? 

Asked by: Image
Thank you for your question. According to the literature and our data in arginase II transfected lysates, the expected molecular weight for arginase II is approximately 40 kda. Unfortunately, we do not have any reference to support a molecular weight of 90 kda in cell lines at this time. Therefore, we are going to remove that data from our website and reevaluate the antibody in different cell lines. This mouse monoclonal antibody, arginase II (A-10): sc-393496, is a mouse monoclonal IgA (kappa light chain). Ideally, we would recommend using an isotype-specific secondary antibody (anti-mouse IgA) for detection. Unfortunately, we do not currently offer this type of secondary antibody for sale.
Answered by: Technical Support
Date published: 2017-11-22

What blocking solution should I use with arginase II (A-10): sc-393496 monoclonal antibody for Western blot?

Asked by: jenni
Thank you for your question. We recommend blocking with Blotto (either TBS Blotto A: sc-2333 or TBS Blotto B: sc-2335) Our Western blot protocol can be found here: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support 5
Date published: 2017-02-09
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Rated 4 out of 5 by from Good resultI got free 10 µg sample of arginase II (A-10): sc-393496 one week ago, and prepared the HPF cell sample immidely, perfect WB result was obtained yesterday, the dilution of this antibody was 1:500, and incubated overnight at 4.
Date published: 2017-09-22
Rated 5 out of 5 by from Good performance， which really help me quite a lottwo weeks ago , I applied the free sample for pre-experiment, THe results turns out to be satisfactory. I really appreciate that. and the delivery service is good, thanks
Date published: 2017-08-17
Rated 5 out of 5 by from A good choice.This antibody has some great data to support it. Immunostaining and IF is bright, clear and specific. The WB data shows strong and specific bands. This antibody is reactive with a broad range of species.
Date published: 2017-01-25
Rated 5 out of 5 by from Good signal strength in human arginase IIGood signal strength in human arginase II transfected 293T whole cell lysate. -SCBT QC
Date published: 2015-04-07
Rated 5 out of 5 by from Works well for IF applicationsWorks well for IF applications, positive in HeLa. -SCBT QC
Date published: 2015-01-02
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Arginase 2/ARG2 抗体 (A-10) is rated 4.8 out of 5 by 5.
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