Date published: 2025-9-7
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ARF6 Antibody (3A-1) - Western Blotting - Image 355307
ARF6 抗体 (3A-1): sc-7971. Hs 181 Tes (A), SP2/0 (B), EOC 20 (C), C6 (D) 和 PC-12 (E) 全细胞裂解液中 ARF6 表达的 Western 印迹分析.
ARF6 抗体 (3A-1): sc-7971. Hs 181 Tes (A), SP2/0 (B), EOC 20 (C), C6 (D) 和 PC-12 (E) 全细胞裂解液中 ARF6 表达的 Western 印迹分析.

ARF6 抗体 (3A-1): sc-7971

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说明书
  • ARF6 抗体 3A-1 是小鼠单克隆 IgG2b κ，ARF6抗体, 在108篇文献中引用，规格为200 µg/ml
  • 针对氨基酸1-174，代表全长ARF6，源自human
  • ARF6 抗体 (3A-1) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 ARF6
  • 抗ARF6抗体（3A-1）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取ARF6 (3A-1): sc-7971的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG2b BP-HRP 和 m-IgGκ BP-HRP 是 ARF6 抗体 (3A-1) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与ARF6 抗体 (3A-1) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 ARF 抗体 包括 ARF1, ARF3, ARF5和ARF6

ARF6抗体（3A-1）是一种小鼠单克隆IgG2b kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、免疫组织化学和酶联免疫吸附测定（ELISA）检测小鼠、大鼠和人源的ARF6蛋白。ARF6 (3A-1) 抗体有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 结合型。ARF6是ADP-核糖基化因子家族的一员，在调节膜运输和细胞骨架动力学方面发挥着至关重要的作用，而膜运输和细胞骨架动力学对于维持细胞内稳态和促进细胞内通讯至关重要。ARF6主要定位于细胞膜和内体腔，负责调控膜蛋白和脂质的回收，从而影响内吞作用和外排作用等过程。这种定位对于细胞迁移、粘附和膜突起形成等各种细胞过程的正常运作至关重要。人类ARF6基因由5个外显子和4个内含子组成，编码一种175个氨基酸的蛋白质，该蛋白质对于协调囊泡运输和维持细胞器完整性至关重要。抗ARF6抗体（3A-1）有助于深入了解细胞信号传导和运输机制，这对于理解各种生理和病理状况至关重要。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

ARF6 抗体 (3A-1) 参考文献:

  1. 活化的 ADP 核糖基化因子在高尔基体膜上形成不同的肌动蛋白池。  |  Fucini, RV., et al. 2000. J Biol Chem. 275: 18824-9. PMID: 10777475
  2. 衣壳复合体的γ-亚基与Cdc42结合，介导转化。  |  Wu, WJ., et al. 2000. Nature. 405: 800-4. PMID: 10866202
  3. 高尔基体囊泡蛋白与由 mAbp1 确定的肌动蛋白复合体的组装有关。  |  Fucini, RV., et al. 2002. Mol Biol Cell. 13: 621-31. PMID: 11854417
  4. ARF6和Rab11是轴突再生的内在调节因子  |  Nieuwenhuis, B. and Eva, R. 2020. Small GTPases. 11: 392-401. PMID: 29772958
  5. Arf6 调节中性粒细胞的能量代谢。  |  Gamara, J., et al. 2021. Free Radic Biol Med. 172: 550-561. PMID: 34245858
  6. 通过破坏 ARF6 介导的分拣系统，靶向表皮生长因子受体依赖性肿瘤。  |  Guo, H., et al. 2022. Nat Commun. 13: 6004. PMID: 36224181
  7. 作为治疗靶点的 Arf6：结构、机制和抑制剂  |  Sun, D., et al. 2023. Acta Pharm Sin B. 13: 4089-4104. PMID: 37799386
  8. 血小板源性细胞外囊泡通过传递 ARF6 加重脓毒症急性肾损伤  |  Lu, X., et al. 2023. Int J Biol Sci. 19: 5055-5073. PMID: 37928258
  9. ADP-ribosylation factor (ARF) 1 的氨基末端是与 Gs 和 ARF GTPase-activating protein 相互作用的必要条件。  |  Randazzo, PA., et al. 1994. J Biol Chem. 269: 29490-4. PMID: 7961931
  10. 人类 ADP-ribosylation 因子 1 与 GDP 复合物的结构。  |  Amor, JC., et al. 1994. Nature. 372: 704-8. PMID: 7990966
  11. 哺乳动物的 Cdc42 是高尔基体的一种布雷非德菌素 A 敏感成分。  |  Erickson, JW., et al. 1996. J Biol Chem. 271: 26850-4. PMID: 8900167
  12. ADP 核糖基化因子调节谱林与高尔基复合体的结合。  |  Godi, A., et al. 1998. Proc Natl Acad Sci U S A. 95: 8607-12. PMID: 9671725

订购信息

产品名称产品编号规格价格数量收藏夹

ARF6 抗体 (3A-1)

sc-7971
200 µg/ml
RMB2377.00

ARF6 (3A-1): m-IgG Fc BP-HRP 套装

sc-528192
200 µg Ab; 10 µg BP
RMB2662.00

ARF6 (3A-1): m-IgGκ BP-HRP 套装

sc-520514
200 µg Ab, 40 µg BP
RMB2662.00

ARF6 (3A-1): m-IgG2b BP-HRP 套装

sc-550006
200 µg Ab; 10 µg BP
RMB2662.00

ARF6 抗体 (3A-1) AC

sc-7971 AC
500 µg/ml, 25% agarose
RMB3129.00

ARF6 抗体 (3A-1) HRP

sc-7971 HRP
200 µg/ml
RMB2377.00

ARF6 抗体 (3A-1) FITC

sc-7971 FITC
200 µg/ml
RMB2482.00

ARF6 抗体 (3A-1) PE

sc-7971 PE
200 µg/ml
RMB2580.00

ARF6 抗体 (3A-1) Alexa Fluor® 488

sc-7971 AF488
200 µg/ml
RMB2685.00

ARF6 抗体 (3A-1) Alexa Fluor® 546

sc-7971 AF546
200 µg/ml
RMB2685.00

ARF6 抗体 (3A-1) Alexa Fluor® 594

sc-7971 AF594
200 µg/ml
RMB2685.00

ARF6 抗体 (3A-1) Alexa Fluor® 647

sc-7971 AF647
200 µg/ml
RMB2685.00

ARF6 抗体 (3A-1) Alexa Fluor® 680

sc-7971 AF680
200 µg/ml
RMB2685.00

ARF6 抗体 (3A-1) Alexa Fluor® 790

sc-7971 AF790
200 µg/ml
RMB2685.00

Do you know if sc-7971 will work for canine samples?

Asked by: TinTin
Thank you for your question. Please contact our Technical Service Department and we would be happy to assist you with performing sequence analysis to determine if this antibody may be suitable for use with canine samples. Please call us directly at (800) 457-3801 option 2, email [email protected], or contact us by live chat.
Answered by: Technical Support
Date published: 2016-12-30
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Rated 5 out of 5 by from works great for WBWorks well for neonatal mouse lungs at the concentration of 30ug of protein. Ab dilution will be 1:1000 for overnight at 4oC
Date published: 2024-12-13
Rated 5 out of 5 by from Works well for WBWorks well on neonatal mouse lungs at concentration of 30ug. Ab dilution will be 1:1000 for overnight @ 4oC.
Date published: 2024-12-10
Rated 1 out of 5 by from Doesn't work for WB293A cells WB does not work. No band showed up at all.
Date published: 2019-05-24
Rated 2 out of 5 by from Antibody seems unspecificI used this antibody on Western Blot. It was working, however it was showing bands even after silencing of Arf6 by 3 different siRNA.
Date published: 2018-02-16
Rated 5 out of 5 by from Great for Western blot with Canine cell lysates.I tested this antibody with my canine stable cell line (MDCK II), it worked well in the western blot, give a very strong and clear band in the expected size.
Date published: 2017-07-21
Rated 5 out of 5 by from High quality antibody for ARF6We tested this antibody In different esophageal squamous cells, barrett's esophagus cells and esophageal adenocarcinoma cells, which showed unique and clear bands at expected ARF6 size (~20KDa). It is a reliable and great antibody for western blot.
Date published: 2017-06-05
Rated 5 out of 5 by from Great KO Data!Highly specific for ARF6, confirmed by CRISPR Double Nickase knockdown samples in Western Blot.
Date published: 2017-01-10
Rated 4 out of 5 by from co-IP assayThis antibody (ARF6 3A-1) can be used for co-IP assay.
Date published: 2016-11-22
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ARF6 抗体 (3A-1) is rated 4.4 out of 5 by 18.
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