Date published: 2025-9-8
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Aquaporin 5/AQP5 Antibody (D-7) - Immunohistochemistry - Image 370163
AQP5 抗体 (D-7): sc-514022. 福尔马林固定, 石蜡包埋的人唾液腺组织的免疫过氧化物酶染色, 显示腺细胞顶端膜和胞浆染色.
AQP5 抗体 (D-7): sc-514022. 福尔马林固定, 石蜡包埋的人唾液腺组织的免疫过氧化物酶染色, 显示腺细胞顶端膜和胞浆染色.

Aquaporin 5/AQP5 抗体 (D-7): sc-514022

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寫評論提問

说明书
  • Aquaporin 5/AQP5 抗体 D-7 是小鼠单克隆 IgG1 κ，Aquaporin 5/AQP5抗体, 在38篇文献中引用，规格为200 µg/ml
  • 免疫原氨基酸序列66-265位于human物种的AQP5的C-terminus
  • Aquaporin 5/AQP5 抗体 (D-7) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 AQP5
  • 抗Aquaporin 5/AQP5抗体（D-7）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Aquaporin 5/AQP5 (D-7): sc-514022的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgGκ BP-HRP 是 Aquaporin 5/AQP5 抗体 (D-7) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Aquaporin 5/AQP5 抗体 (D-7) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 AQP 抗体 包括 AQP0, AQP1, AQP2, AQP3, AQP4, AQP5, AQP7, AQP8和AQP9

AQP5抗体（D-7）是一种IgG1 κ小鼠单克隆AQP5抗体（也称为水通道蛋白5抗体），可通过WB, IP, IF, IHC(P) 和 ELISA检测小鼠、大鼠和人类来源的AQP5蛋白。AQP5抗体（D-7）既有非偶联抗AQP5抗体形式，也有多种偶联形式的抗AQP5抗体，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®偶联物。水通道蛋白（AQPs）是一大类整体膜水通道蛋白，可促进水通过细胞膜的运输。这种功能在动物、植物和细菌中是保守的。在哺乳动物中已经鉴定出了许多种水通道蛋白的同工型，分别命名为AQP0到AQP10。水通道蛋白分布广泛，同一细胞中存在多种AQP的情况并不少见。尽管大多数水通道蛋白仅对水具有通透性，但AQP3、AQP7、AQP9和两种AQP10转录本中的一种也对尿素和甘油具有通透性。AQP2是唯一一种由抗利尿激素激活的水通道，可增强肾集合管中的水再吸收。水通道蛋白参与肾水吸收、肺分泌物生成、泪液分泌以及脑脊液和房水的分泌与再吸收。在肺中，AQP5负责大部分水通过I型肺泡上皮细胞顶端膜的运输。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Aquaporin 5/AQP5 抗体 (D-7) 参考文献:

  1. 哺乳动物水通道蛋白家族：有希望的新药靶点  |  Beitz, E. and Schultz, JE. 1999. Curr Med Chem. 6: 457-67. PMID: 10213793
  2. 水通道蛋白-5基因敲除小鼠的肺液运输  |  Ma, T., et al. 2000. J Clin Invest. 105: 93-100. PMID: 10619865
  3. 分离出 28 千道尔顿的红细胞整体膜蛋白 cDNA：古老通道家族的成员。  |  Preston, GM. and Agre, P. 1991. Proc Natl Acad Sci U S A. 88: 11110-4. PMID: 1722319
  4. AQP5富集远端胃中的干细胞和癌源。  |  Tan, SH., et al. 2020. Nature. 578: 437-443. PMID: 32025032
  5. AQP5与LGR5共同通过调节ULK1泛素化来决定胃癌干细胞的命运。  |  Zhao, R., et al. 2022. J Exp Clin Cancer Res. 41: 322. PMID: 36372898
  6. NNMT富集AQP5+癌干细胞，从而促进早期胃卡氏腺癌的恶性进展。  |  Wang, Z., et al. 2023. Gut. 73: 63-77. PMID: 36977555
  7. AQP5缺乏通过线粒体功能障碍促进晶状体上皮细胞的衰老。  |  Zhang, K., et al. 2023. Biochem Biophys Res Commun. 680: 184-193. PMID: 37742347
  8. 人类水通道（AQP3）基因的结构和染色体定位。  |  Ishibashi, K., et al. 1995. Genomics. 27: 352-4. PMID: 7558005
  9. 人类汞不敏感水通道的 cDNA 克隆, 基因组织和染色体定位。不同转录单元的证据。  |  Yang, B., et al. 1995. J Biol Chem. 270: 22907-13. PMID: 7559426
  10. 人体肾脏水通道 aquaporin-2 对血管加压素依赖性尿液浓缩的要求。  |  Deen, PM., et al. 1994. Science. 264: 92-5. PMID: 8140421
  11. 人类 Aquaporin-5 基因。分子特征和染色体定位。  |  Lee, MD., et al. 1996. J Biol Chem. 271: 8599-604. PMID: 8621489
  12. 水通道蛋白。  |  Echevarría, M. and Ilundáin, AA. 1998. J Physiol Biochem. 54: 107-18. PMID: 9858131

订购信息

产品名称产品编号规格价格数量收藏夹

Aquaporin 5/AQP5 抗体 (D-7)

sc-514022
200 µg/ml
RMB2377.00

Aquaporin 5/AQP5 (D-7): m-IgG Fc BP-HRP 套装

sc-531004
200 µg Ab; 10 µg BP
RMB2662.00

Aquaporin 5/AQP5 (D-7): m-IgGκ BP-HRP 套装

sc-524575
200 µg Ab, 40 µg BP
RMB2662.00

Aquaporin 5/AQP5 抗体 (D-7) AC

sc-514022 AC
500 µg/ml, 25% agarose
RMB3129.00

Aquaporin 5/AQP5 抗体 (D-7) HRP

sc-514022 HRP
200 µg/ml
RMB2377.00

Aquaporin 5/AQP5 抗体 (D-7) FITC

sc-514022 FITC
200 µg/ml
RMB2482.00

Aquaporin 5/AQP5 抗体 (D-7) PE

sc-514022 PE
200 µg/ml
RMB2580.00

Aquaporin 5/AQP5 抗体 (D-7) Alexa Fluor® 488

sc-514022 AF488
200 µg/ml
RMB2685.00

Aquaporin 5/AQP5 抗体 (D-7) Alexa Fluor® 546

sc-514022 AF546
200 µg/ml
RMB2685.00

Aquaporin 5/AQP5 抗体 (D-7) Alexa Fluor® 594

sc-514022 AF594
200 µg/ml
RMB2685.00

Aquaporin 5/AQP5 抗体 (D-7) Alexa Fluor® 647

sc-514022 AF647
200 µg/ml
RMB2685.00

Aquaporin 5/AQP5 抗体 (D-7) Alexa Fluor® 680

sc-514022 AF680
200 µg/ml
RMB2685.00

Aquaporin 5/AQP5 抗体 (D-7) Alexa Fluor® 790

sc-514022 AF790
200 µg/ml
RMB2685.00

Would it be possible to obtain the sequence of the antibody / where the antibody binds AQP5 (extracellular domain / intracellular domain)?

Asked by: DoctorDR
Thank you for your question. The antibody sequence is proprietary and the antibody detect the intecellular domain.
Answered by: BlakeJ
Date published: 2023-08-09

Is this antibody also suitable and tested for Flow cytometrie? And is there a recommended dilution?

Asked by: bco27
Thank you for your question. Anti-AQP5 Antibody (D-7) is recommended for detection of AQP5 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA. We also offer fluorescence-conjugated versions of this antibody for use in flow cytometry at 1 µg of fluorochrome-conjugate for each tube of 100 µl cell suspension (equal to 1 million cells).
Answered by: Tech Support Europe
Date published: 2021-03-05

请问北京的用户想买贵公司的产品该如何联系你们？有没有电话？

Asked by: y yang
感谢您的提问。您需要订购可直接联系我们的技术支持部门（上海），亚洲技术支持电话(+86) 021-60936350、邮箱[email protected]、或选择网页上Live Chat中的“中文支持”。您也可以联系我们北京的经销商， https://www.scbt.com/scbt/resources/distributors Thank you for your inquiry. You can contact our Technical Service Department in shanghai for ordering, our Asian Technical Service team is available at (+86) 021-60936350 or [email protected] or by live chat on our website. In addition, you can also contact our distributors in Beijing, https://www.scbt.com/scbt/resources/distributors
Answered by: Technical Support 15
Date published: 2017-08-24

How to get the free demo antibody?  I want to test about AQP5 抗体 (D-7): sc-514022,Aquaporin-5

Asked by: cqm0556
Thank you for your interest in testing a free sample of this antibody. Please call our Asian Support office toll-free at 800-820-8626, email [email protected], or contact us by live chat to request a free 10 µg sample.
Answered by: Technical Support
Date published: 2017-07-25

Can AQP5 (D-7): sc-514022 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: Dr Ninau Qelp
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, AQP5 (D-7): sc-514022 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from FunzionaL’anticorpo è stato utilizzando per IHC e IF su tessuto, incubazione o.n., diluizioni 1:50 1:100 e 1:150. E’ stato rilevato il segnale con localizzazione in membrana alle diluizioni 1:50 e 1:100.
Date published: 2021-09-28
Rated 5 out of 5 by from Very usefulThis antibody is very useful for detect AQP5 in colorectal carcinoma tissue , we found that the AQP5 protein is very abundant in colorectal carcinoma tissue.We detect it by WB, loading 30ug sample, got strong signal at 1:400 dilution
Date published: 2017-09-09
Rated 1 out of 5 by from IHC Assay Review for AQP5 mABApplication: Immunohistochemistry. Protocol: Standard protocol for saliva smear staining. Dilutions tested: 1:10, 1:30, 1:90, 1:270. Results: Minimal staining at dilution factor 10, unclear if specific.
Date published: 2017-08-31
Rated 5 out of 5 by from WB analysisDetecion for WB at 1:1000 dilution 293TT cells. This antibody showed specific bands for AQP5 at the correct size.
Date published: 2017-08-26
Rated 5 out of 5 by from 一次就出结果了我是申请试用的抗体，量还比较足，检测的样本是动物组织，仪器使用的BIO-RAD,转膜条件是200mA,时间90min, 5%脱脂奶粉封闭，1%BSA配制一抗，比例为1：500，4度过夜，二抗是millipore 1:20000, millipore ECL发光液显影。图片无法上传，我的是PDF格式
Date published: 2017-08-04
Rated 5 out of 5 by from AQP5 analysisThis antibody detected strong signal,I suggest increase the antibody conceration to 1:400 and concentrat sample to load 50ug,I got specific band at 35kda.
Date published: 2017-07-22
Rated 5 out of 5 by from Good antibody for Western blotsI used this antibody at a 1:1000 dilution, which gives a strong band ~37 kDa.
Date published: 2017-07-20
Rated 5 out of 5 by from good antibodyThe antibody is good quality, good reproducibility, and no background,we tried different dilutions, and finally optimized at 1:500.
Date published: 2017-07-15
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Aquaporin 5/AQP5 抗体 (D-7) is rated 4.1 out of 5 by 15.
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