Date published: 2025-9-8
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Aquaporin 4/AQP4 Antibody (4/18) - Immunohistochemistry - Image 11433
AQP4 抗体 (4/18): sc-32739. 福尔马林固定, 石蜡包埋的大鼠脑组织的免疫过氧化物酶染色, 显示膜定位.
Aquaporin 4/AQP4 Antibody (4/18) - Immunohistochemistry - Image 11433
AQP4 抗体 (4/18): sc-32739. 福尔马林固定, 石蜡包埋的大鼠脑组织的免疫过氧化物酶染色, 显示膜定位.
Aquaporin 4/AQP4 Antibody (4/18) - Immunofluorescence - Image 9225
AQP4 抗体 (4/18): sc-32739. 大鼠大脑皮层的免疫荧光染色. 由匈牙利佩奇大学的 Nagy G. 慷慨提供.
Aquaporin 4/AQP4 Antibody (4/18) - Flow Cytometry - Image 12355
AQP4 抗体 (4/18): sc-32739. 用 AQP4 抗体 (4/18), 然后用 PE-conjugated 山羊抗小鼠 IgG3: sc-3767 染色的固定和通透的 Hep G2 细胞的间接细胞内 FCM 分析. 黑线直方图代表同型对照--正常小鼠 IgG3: sc-3880.
AQP4 抗体 (4/18): sc-32739. 福尔马林固定, 石蜡包埋的大鼠脑组织的免疫过氧化物酶染色, 显示膜定位.

Aquaporin 4/AQP4 抗体 (4/18): sc-32739

4.0(21)
寫評論提問

说明书
  • Aquaporin 4/AQP4 抗体 4/18 是小鼠单克隆 IgG3 κ，Aquaporin 4/AQP4抗体, 在43篇文献中引用，规格为200 µg/ml
  • 免疫human物种的intracellular AQP4的氨基酸301-318
  • 推荐用于 mouse, rat 和 human 来源的AQP4 WB, IP, IF, IHC(P) 和 FCM检测
  • 还可偶联琼脂糖 用于IP; 偶联藻红蛋白,FITC, Alexa Fluor® 488或Alexa Fluor® 647的产品, 应用于IF, IHC(P)和FCM
  • 关于如何获取Aquaporin 4/AQP4 (4/18): sc-32739的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前，我们还没有完成Aquaporin 4/AQP4 抗体 (4/18)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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相关产品
描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 AQP 抗体 包括 AQP0, AQP1, AQP2, AQP3, AQP4, AQP5, AQP7, AQP8和AQP9

AQP4抗体（4/18）是一种小鼠单克隆IgG3 kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、免疫组织化学和流式细胞术（FCM）检测小鼠、大鼠和人类来源的AQP4蛋白。抗AQP4抗体（4/18）有非结合型和多种结合型，包括琼脂糖、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。AQP4，也称为水通道蛋白4，在细胞膜的水分运输中起着至关重要的作用，特别是在中枢神经系统和骨骼肌中。AQP4主要定位于快速收缩肌纤维的肌膜上，在那里AQP4促进水分快速流动，这对肌肉功能和水分补充至关重要。这种定位有助于维持渗透平衡，并在剧烈运动时支持肌肉收缩。水通道蛋白（包括AQP4）是组成膜蛋白，可形成允许水选择性渗透的通道，水通道蛋白功能障碍可导致多种病理生理状况，包括水肿和神经系统疾病。多种水通道蛋白同工型在组织中的存在凸显了它们在多种生理过程（如肾脏吸水和对脑脊液动力学的调节）中的重要性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Aquaporin 4/AQP4 抗体 (4/18) 参考文献:

  1. 哺乳动物水通道蛋白家族：有希望的新药靶点  |  Beitz, E. and Schultz, JE. 1999. Curr Med Chem. 6: 457-67. PMID: 10213793
  2. 分离出 28 千道尔顿的红细胞整体膜蛋白 cDNA：古老通道家族的成员。  |  Preston, GM. and Agre, P. 1991. Proc Natl Acad Sci U S A. 88: 11110-4. PMID: 1722319
  3. 人类水通道（AQP3）基因的结构和染色体定位。  |  Ishibashi, K., et al. 1995. Genomics. 27: 352-4. PMID: 7558005
  4. 人类汞不敏感水通道的 cDNA 克隆, 基因组织和染色体定位。不同转录单元的证据。  |  Yang, B., et al. 1995. J Biol Chem. 270: 22907-13. PMID: 7559426
  5. 人体肾脏水通道 aquaporin-2 对血管加压素依赖性尿液浓缩的要求。  |  Deen, PM., et al. 1994. Science. 264: 92-5. PMID: 8140421
  6. 人类 Aquaporin-5 基因。分子特征和染色体定位。  |  Lee, MD., et al. 1996. J Biol Chem. 271: 8599-604. PMID: 8621489
  7. 小鼠 aquaporin 4 的克隆和染色体定位：排除共济失调这一候选突变表型。  |  Turtzo, LC., et al. 1997. Genomics. 41: 267-70. PMID: 9143504
  8. 哺乳动物骨骼肌快肌纤维中水蒸素-4的表达。  |  Frigeri, A., et al. 1998. J Clin Invest. 102: 695-703. PMID: 9710437
  9. 水通道蛋白。  |  Echevarría, M. and Ilundáin, AA. 1998. J Physiol Biochem. 54: 107-18. PMID: 9858131

订购信息

产品名称产品编号规格价格数量收藏夹

Aquaporin 4/AQP4 抗体 (4/18)

sc-32739
200 µg/ml
RMB2377.00

Aquaporin 4/AQP4 抗体 (4/18) AC

sc-32739 AC
500 µg/ml, 25% agarose
RMB3129.00

Aquaporin 4/AQP4 抗体 (4/18) Alexa Fluor® 488

sc-32739 AF488
200 µg/ml
RMB2685.00

Aquaporin 4/AQP4 抗体 (4/18) Alexa Fluor® 647

sc-32739 AF647
200 µg/ml
RMB2685.00

Aquaporin 4/AQP4 抗体 (4/18) FITC

sc-32739 FITC
200 µg/ml
RMB2482.00

Aquaporin 4/AQP4 抗体 (4/18) PE

sc-32739 PE
200 µg/ml
RMB2580.00

Hi, does this antibody require permeabilization for staining astrocytes by flow?

Asked by: JJY2
Thank you for your question. Please see permeabilzation steps in our protocol on section C : https://www.scbt.com/resources/protocols/flow-cytometry
Answered by: BlakeJ
Date published: 2024-11-05

I am currently using your AQP4 antibody (sc-32739) in western blots and found that ECL results showed bands at ~70kDa and fluorescence showed bands at ~35 kDa. Do you know why this might be the case?

Asked by: greeny5
Thank you for your question. For assistance please contact technical service by email [email protected] or phone (800)-457-3801.
Answered by: BlakeJ
Date published: 2023-06-07

I am trying IHC on mouse tissue sections. Which secondary antibody would you recommend? H+L or IgG3 specific? This antibody also gives a lot of bands in western blot around 40 kDa, not exactly around the expected size of the AQP4 isoforms.

Asked by: Tejal A
Thank you for your question. It would be helpful if you could call 800-457-3801, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Service
Date published: 2022-04-07

I am a PhD student at the university of Washington. We are looking for a good antibody for mouse kidney and brain sections and wondering if you could offer a free aliquot of AQP4 Antibody (sc-32739) for us to test first.

Asked by: Chenyi
Thank you for your question. Please contact your local distributor or technical service for further assistance.
Answered by: Dallas Tech Service
Date published: 2021-04-20

This is from Johns hopkins University. We plan to order AQP4 Antibody (4/18): sc-32739. First we want to make sure if the antibody can arrived tomorrow if we ordered today? Thanks!

Asked by: Anonymous
Thank you for your question. Yes, we ship all our antibody reagents overnight, so if you order a vial of sc-32739 today, you will receive it tomorrow. It is currently in stock and ready to ship to you!
Answered by: Tech Service
Date published: 2019-11-11

We used it to test for IF on frozen rat brain tissue by Paraformaldehyde perfusion ,  but  there is no positive expression.

Asked by: h123
Thank you for your question. This mouse monoclonal, AQP4 (4/18): sc-32739, is suitable for that research application. We recommend using the primary antibody at a starting dilution of 1:50 (range of 1:50-1:500). And since you are using Paraformaldehyde, we recommend including a cell permeabilization treatment (e.g., Triton X-100). A link to our recommended protocol is below. This antibody has also been cited in the literature for IF on rat brain tissue. As one example, please refer to the following publication on NCBI (PMID: 22420318). https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining Please contact our Technical Support Department for additional support. Please call our Asian Support office toll-free at 800-820-8626, email [email protected], or contact us by live chat.
Answered by: Technical Support
Date published: 2017-02-14

Why does your Western blot data show doublet bands with AQP4 (4/18): sc-32739 monoclonal antibody?

Asked by: jenni
AQP4 consists of several phosphorylation and glycosylation sites, therefore, the doublet bands that appear on our Western blot may be due to post-translational modifications of AQP4.
Answered by: Technical Support 15
Date published: 2019-01-28

Can AQP4 (4/18): sc-32739 this antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: jenni
Thank you for your question. Yes, AQP4 (4/18): sc-32739 monoclonal antibody has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support 15
Date published: 2017-01-12
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Rated 5 out of 5 by from Ottimo anticorpoHo recentemente provato questo anticorpo in IF, su fettine di midollo spinale di topo criostatate. Diluizione 1:200 e funziona bene, il segnale è pulito e specifico.Ottimo prodotto. Funziona molto bene anche per co-immuno
Date published: 2019-09-18
Rated 1 out of 5 by from 小鼠脑片AQP4一直染不出来我按照1：50的浓度染，没有；按照1：25染还是没有。用枸橼酸钠修复了，triton通透了，但还是没有
Date published: 2018-08-11
Rated 4 out of 5 by from work well in WB at 1:200, with hippocampuswork well in WB at 1:200, with hippocampus,but degrade in storage of -20°C
Date published: 2018-05-07
Rated 1 out of 5 by from Not working on Western blotTrial antibody did not work by Western blot on primary rat astrocyte samples. Blank blots only regardless of antibody dilution, or blocking buffer.
Date published: 2018-04-10
Rated 1 out of 5 by from Didn't work in hek293TUsed AQP4 at a 1:200 dilution for WB on NuPAGE gel using HEK293T WCE. Unfortunately, we weren't able to detect anything.
Date published: 2017-09-29
Rated 5 out of 5 by from 研究血脑屏障以前用过，WB挺好的，感觉SANTA的抗体染色一直都非常好，就是不知道为啥好多抗体停产了，太可惜了。
Date published: 2017-07-28
Rated 5 out of 5 by from Nice for flow cytommetryIt worked very well for flow cytommetry. You can see a nice shift after permeabilisation with Saponin.
Date published: 2017-07-06
Rated 3 out of 5 by from no expression of AQP4 in bEND3 cellsI used this antibody sample at 1/500 dilution to detect AQP4 in bEND3 cell lysate and detectd no bands between 35 and 40 kD.
Date published: 2017-06-20
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Aquaporin 4/AQP4 抗体 (4/18) is rated 4.0 out of 5 by 21.
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