Date published: 2025-9-7
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Aquaporin 1/AQP1 Antibody (B-11) - Western Blotting - Image 375325
AQP1 抗体 (B-11): sc-25287. 小鼠肺 (A), 小鼠肾 (B) 和人肾 (C) 组织提取物中 AQP1 表达的近红外 Western 印迹分析. 使用 UltraCruz® 阻断试剂: sc-516214. 所用检测试剂: m-IgGκ BP-CFL 680: sc-516180.
AQP1 抗体 (B-11): sc-25287. 小鼠肺 (A), 小鼠肾 (B) 和人肾 (C) 组织提取物中 AQP1 表达的近红外 Western 印迹分析. 使用 UltraCruz® 阻断试剂: sc-516214. 所用检测试剂: m-IgGκ BP-CFL 680: sc-516180.

Aquaporin 1/AQP1 抗体 (B-11): sc-25287

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寫評論提問

说明书
  • Aquaporin 1/AQP1 抗体 B-11 是小鼠单克隆 IgG1 κ，Aquaporin 1/AQP1抗体, 在125篇文献中引用，规格为200 µg/ml
  • 免疫human物种的AQP1的氨基酸215-269
  • Aquaporin 1/AQP1 抗体 (B-11) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 AQP1
  • 抗Aquaporin 1/AQP1抗体（B-11）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Aquaporin 1/AQP1 (B-11): sc-25287的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 Aquaporin 1/AQP1 抗体 (B-11) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Aquaporin 1/AQP1 抗体 (B-11) 组合成套装提供。 (请参阅下面的订购信息)。

快捷链接

相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

此外 AQP 抗体 包括 AQP0, AQP1, AQP2, AQP3, AQP4, AQP5, AQP7, AQP8和AQP9

AQP1抗体（B-11）是一种小鼠单克隆IgG1 kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、免疫组织化学和酶联免疫吸附测定（ELISA）检测小鼠、大鼠和人类来源的AQP1蛋白。抗AQP1抗体（B-11）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。AQP1，又称水通道蛋白1，是一种跨膜蛋白，有助于水在细胞膜之间的运输，对于维持渗透平衡和细胞正常功能至关重要。AQP1在红细胞和肾小管细胞中的表达量最高，有助于肾脏吸水并调节体液平衡。AQP1在这些部位的表达表明其在生理过程中发挥着关键作用，例如尿液浓缩和维持血容量。作为水通道蛋白家族的一员，AQP1与各种同工型蛋白协同工作，运输其他小分子溶质，在细胞水合作用和营养运输中发挥着多种作用。AQP1 (B-11) 单克隆抗体是研究人员在各种生物学背景下研究水运输机制和相关生理过程的重要工具。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Aquaporin 1/AQP1 抗体 (B-11) 参考文献:

  1. 哺乳动物水通道蛋白家族：有希望的新药靶点  |  Beitz, E. and Schultz, JE. 1999. Curr Med Chem. 6: 457-67. PMID: 10213793
  2. 分离出 28 千道尔顿的红细胞整体膜蛋白 cDNA：古老通道家族的成员。  |  Preston, GM. and Agre, P. 1991. Proc Natl Acad Sci U S A. 88: 11110-4. PMID: 1722319
  3. 人类疾病中的AQP1表达分析：对蛋白质组学表征的影响。  |  Magni, F., et al. 2008. Expert Rev Proteomics. 5: 29-43. PMID: 18282122
  4. 从红细胞和肾小管中鉴定, 纯化和部分表征一种新的 28,000 摩尔积分膜蛋白。  |  Denker, BM., et al. 1988. J Biol Chem. 263: 15634-42. PMID: 3049610
  5. 小鼠AQP1基因敲除的生理和病理影响。  |  Hua, Y., et al. 2019. Biosci Rep. 39: PMID: 31023968
  6. AQP1调节肌腱衰老过程中的肌腱干细胞/祖细胞衰老。  |  Chen, M., et al. 2020. Cell Death Dis. 11: 193. PMID: 32188840
  7. 人类水通道（AQP3）基因的结构和染色体定位。  |  Ishibashi, K., et al. 1995. Genomics. 27: 352-4. PMID: 7558005
  8. 人类汞不敏感水通道的 cDNA 克隆, 基因组织和染色体定位。不同转录单元的证据。  |  Yang, B., et al. 1995. J Biol Chem. 270: 22907-13. PMID: 7559426
  9. 人体肾脏水通道 aquaporin-2 对血管加压素依赖性尿液浓缩的要求。  |  Deen, PM., et al. 1994. Science. 264: 92-5. PMID: 8140421
  10. 人类水蒸气素-CHIP 基因。结构, 组织和染色体定位。  |  Moon, C., et al. 1993. J Biol Chem. 268: 15772-8. PMID: 8340403
  11. 人类 Aquaporin-5 基因。分子特征和染色体定位。  |  Lee, MD., et al. 1996. J Biol Chem. 271: 8599-604. PMID: 8621489
  12. 水通道蛋白。  |  Echevarría, M. and Ilundáin, AA. 1998. J Physiol Biochem. 54: 107-18. PMID: 9858131

订购信息

产品名称产品编号规格价格数量收藏夹

Aquaporin 1/AQP1 抗体 (B-11)

sc-25287
200 µg/ml
RMB2377.00

Aquaporin 1/AQP1 (B-11): m-IgG Fc BP-HRP 套装

sc-528395
200 µg Ab; 10 µg BP
RMB2662.00

Aquaporin 1/AQP1 (B-11): m-IgGκ BP-HRP 套装

sc-520766
200 µg Ab, 40 µg BP
RMB2662.00

Aquaporin 1/AQP1 (B-11): m-IgG1 BP-HRP 套装

sc-542913
200 µg Ab; 20 µg BP
RMB2662.00

Aquaporin 1/AQP1 抗体 (B-11) AC

sc-25287 AC
500 µg/ml, 25% agarose
RMB3129.00

Aquaporin 1/AQP1 抗体 (B-11) HRP

sc-25287 HRP
200 µg/ml
RMB2377.00

Aquaporin 1/AQP1 抗体 (B-11) FITC

sc-25287 FITC
200 µg/ml
RMB2482.00

Aquaporin 1/AQP1 抗体 (B-11) PE

sc-25287 PE
200 µg/ml
RMB2580.00

Aquaporin 1/AQP1 抗体 (B-11) Alexa Fluor® 488

sc-25287 AF488
200 µg/ml
RMB2685.00

Aquaporin 1/AQP1 抗体 (B-11) Alexa Fluor® 546

sc-25287 AF546
200 µg/ml
RMB2685.00

Aquaporin 1/AQP1 抗体 (B-11) Alexa Fluor® 594

sc-25287 AF594
200 µg/ml
RMB2685.00

Aquaporin 1/AQP1 抗体 (B-11) Alexa Fluor® 647

sc-25287 AF647
200 µg/ml
RMB2685.00

Aquaporin 1/AQP1 抗体 (B-11) Alexa Fluor® 680

sc-25287 AF680
200 µg/ml
RMB2685.00

Aquaporin 1/AQP1 抗体 (B-11) Alexa Fluor® 790

sc-25287 AF790
200 µg/ml
RMB2685.00

I wanted to know if this antibody can work for flow cytometry. If this cannot work in flow, can you suggest any other AQP1 conjugated antibody which will be work for flow cytometry. Thanks!

Asked by: Sudhanshu
Thank you for your question. Aquaporin 1/AQP1 Antibody (1/22): sc-32737 is recommended for detection of AQP1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and FCM.
Answered by: Tech Support Europe
Date published: 2024-10-15

Can I use AQP1 (B-11) for immunofluorescence staining of mice renal tissue? If so, is there a recommended dilution?

Asked by: Loz2
Thank you for your question. This mouse monoclonal antibody, AQP1 (B-11): sc-25287, is recommended for immunofluorescence staining (IF) of mouse tissues. We recommend a starting dilution of 1:50 (range of 1:50-1:500). A link to our recommended protocol is provided below as a reference. https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-10-02

Can I use AQP1 (B-11): sc-25287 mouse monoclonal antibody for immunohistochemical staining of mouse tissue?

Asked by: jenni
Yes. We recommend using a directly conjugated monoclonal antibody. AQP1 (B-11): sc-25287 monoclonal antibody is available directly conjugated to either HRP (sc-25287 HRP), phycoerythrin (sc-25287 PE), fluorescein (sc-25287 FITC), Alexa Fluor® 488 (sc-25287 AF488) or Alexa Fluor® 647 (sc-25287 AF647)
Answered by: Technical Support 4
Date published: 2017-01-09
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Rated 1 out of 5 by from didnot work for formalin fixed kideny tissue IFI tried this antibody several times for IF of formalin fixed kidney, unfortunately, it dino not work.
Date published: 2025-07-13
Rated 4 out of 5 by from Works fineClear band visible ~40 kDa when used at 1:200 in 2% Milk (ON 4 degrees, gradient gel 4-15%).
Date published: 2020-01-02
Rated 5 out of 5 by from very goodAQP1 (B-11): sc-25287. tested using in adipose tissue homogenate enriched in capillaries. Antibody dilution: 1 : 250.
Date published: 2018-10-30
Rated 5 out of 5 by from work well in WB at 1:200, with hippocampuswork well in WB at 1:200, with hippocampus,i like this
Date published: 2018-05-10
Rated 5 out of 5 by from Good Antibody - SpecificI got a clean band at the right size for HK2 cells
Date published: 2018-02-05
Rated 4 out of 5 by from Working fine in WBThis AQP1 antibody is working fine in WB with human liver, mouse liver, kidney, brain and hippocampus. However, it does not recognize human origin of AQP1.
Date published: 2017-10-12
Rated 4 out of 5 by from Aqp1Great western results in ES mouse cells; strong, specific bands.
Date published: 2017-10-03
Rated 4 out of 5 by from Good productI bought this antibody for human primary TM cells staining. I used 1 in 100 duilution and it was worked well in my case.
Date published: 2017-09-11
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Aquaporin 1/AQP1 抗体 (B-11) is rated 4.6 out of 5 by 20.
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