Date published: 2025-9-11
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apolipoprotein E/apoE Antibody (WU E-4 ) - Western Blotting - Image 352742
载脂蛋白 E 抗体 (WU E-4): sc-53570. 人肝脏组织提取物 (A) 和 Hep G2 全细胞裂解液 (B) 中载脂蛋白表达的 Western 印迹分析.
载脂蛋白 E 抗体 (WU E-4): sc-53570. 人肝脏组织提取物 (A) 和 Hep G2 全细胞裂解液 (B) 中载脂蛋白表达的 Western 印迹分析.

apolipoprotein E/apoE 抗体 (WU E-4): sc-53570

4.8(8)
寫評論提問

说明书
  • apolipoprotein E/apoE抗体(WU E-4)是小鼠单克隆IgG1 κ, 在13篇文献中引用，规格为200 µg/ml
  • 抗human起源的ApoE
  • 抗-apolipoprotein E/apoE 抗体 (WU E-4) 推荐用于 WB, IP, IF 和 IHC(P)，检测mouse, rat 和human 来源的 apoE
  • 抗apolipoprotein E/apoE抗体（WU E-4）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取apolipoprotein E/apoE (WU E-4): sc-53570的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG1 BP-HRP 是 apolipoprotein E/apoE 抗体 (WU E-4) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与apolipoprotein E/apoE 抗体 (WU E-4) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 Apolipoprotein 抗体 包括 apoA-I, apoA-II, apoA-IV, apoA-V, apoC-I, apoC-II, apoC-III, apoC-IV, apoL1和apoL3

apoE 抗体（WU E-4）是一种小鼠单克隆 IgG1 kappa 轻链抗体，可特异性靶向来自人、小鼠和大鼠的载脂蛋白 E（apolipoprotein E，载脂蛋白 E）。载脂蛋白 E 是一种重要的蛋白质，存在于细胞的不同位置，包括质膜和细胞膜。载脂蛋白 E 存在于血浆脂蛋白中，对脂蛋白颗粒的结合、内化和分解具有重要意义，因此载脂蛋白 E 对胆固醇和脂质的平衡至关重要。作为低密度脂蛋白受体和肝脏载脂蛋白受体等受体的配体，载脂蛋白有助于清除富含胆固醇的脂蛋白，从而影响心血管健康。载脂蛋白在许多器官中合成，包括肝脏和大脑，并存在于多种脂蛋白组分中，包括 VLDL 和 HDL。重要的是，载脂蛋白有多种异构体，特别是 E2、E3 和 E4，它们在脂质代谢和神经退行性疾病中都有功能性影响。建议将载脂蛋白（WU E-4）抗体用于免疫印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和石蜡包埋切片免疫组化（IHCP）等应用，为研究人员提供研究载脂蛋白在脂质转运和细胞信号传导中作用的可靠工具。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

apolipoprotein E/apoE 抗体 (WU E-4)

sc-53570
200 µg/ml
RMB2377.00

apolipoprotein E/apoE (WU E-4): m-IgG Fc BP-HRP 套装

sc-526828
200 µg Ab; 10 µg BP
RMB2662.00

apolipoprotein E/apoE (WU E-4): m-IgG1 BP-HRP 套装

sc-532201
200 µg Ab; 20 µg BP
RMB2662.00

apolipoprotein E/apoE 抗体 (WU E-4) AC

sc-53570 AC
500 µg/ml, 25% agarose
RMB3129.00

apolipoprotein E/apoE 抗体 (WU E-4) HRP

sc-53570 HRP
200 µg/ml
RMB2377.00

apolipoprotein E/apoE 抗体 (WU E-4) FITC

sc-53570 FITC
200 µg/ml
RMB2482.00

apolipoprotein E/apoE 抗体 (WU E-4) PE

sc-53570 PE
200 µg/ml
RMB2580.00

apolipoprotein E/apoE 抗体 (WU E-4) Alexa Fluor® 488

sc-53570 AF488
200 µg/ml
RMB2685.00

apolipoprotein E/apoE 抗体 (WU E-4) Alexa Fluor® 546

sc-53570 AF546
200 µg/ml
RMB2685.00

apolipoprotein E/apoE 抗体 (WU E-4) Alexa Fluor® 594

sc-53570 AF594
200 µg/ml
RMB2685.00

apolipoprotein E/apoE 抗体 (WU E-4) Alexa Fluor® 647

sc-53570 AF647
200 µg/ml
RMB2685.00

apolipoprotein E/apoE 抗体 (WU E-4) Alexa Fluor® 680

sc-53570 AF680
200 µg/ml
RMB2685.00

apolipoprotein E/apoE 抗体 (WU E-4) Alexa Fluor® 790

sc-53570 AF790
200 µg/ml
RMB2685.00

Can this antibody work for immunoprecipitation (IP)?

Asked by: leo123
-thank you for your question. Anti-apolipoprotein E/apoE Antibody (WU E-4) is recommended for detection of apoE of mouse, rat and human origin by immunoprecipitation as well as WB, IF and IHC(P)
Answered by: Tech Support Europe
Date published: 2025-05-09

Which APOE epitope is recognized by this antibody (WU E-4)?

Asked by: Suzie
Thank you for your question. The exact epitope for this monoclonal antibody has not yet been epitope mapped, so we do not have this information. It was raised against purified human ApoE of human origin. Please contact our Technical Service Department and we would be glad to discuss the reactivity of this antibody. You can reach us by email at [email protected], or use the live chat on our website.
Answered by: Tech Service
Date published: 2017-06-13

Was bedeutet FL bei der Beschreibung des Epitops für diesen AK?

Asked by: Suzie
Vielen Dank für Ihre Anfrage und Ihr Interesse an diesem Produkt. Der apoE Antikörper (WU E-4): sc-53570 wurde gegen das humane ApoE Volllängeprotein gezogen. Die Bezeichnung FL steht in diesem Fall für "full length". Für weitere Informationen wenden Sie sich bitte an unseren Technischen Service in unserem Europäischen Supportbüro in Heidelberg. Sie erreichen uns telefonisch unter 06221 4503 0, per email an: [email protected] oder per Live-Chat auf unserer Webseite www.scbt.com Wir freuen uns Ihnen weiterzuhelfen.
Answered by: Technical Support Europe
Date published: 2017-05-18

What is the recommended starting dilution for immunostaining with apoE (WU E-4): sc-53570 monoclonal antibody?

Asked by: jenniferc
Thank you for your question. The recommended starting dilution for immunostaining is 1:50 with a dilution range of 1:50-1:500. This information, along with other technical specifications, can be found in the “datasheet” link at the top of the product page. If you have any further questions or concerns, please contact our Technical Service department by calling 800-457-3801 option 2, emailing [email protected], or using the Live Chat function on our website.
Answered by: Technical Support 5
Date published: 2017-01-20
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Rated 5 out of 5 by from ApoE antibody for western blot application.The antibody worked as described. Price is reasonable. Shipment is fast.
Date published: 2025-02-28
Rated 5 out of 5 by from Excellent detection of apoE by WB and ICCThis antibody showed excellent signal for all three major isoforms of apoE by WB and ICC in murine, rodent and human cell lines. It appears to be specific for the N-terminus of apoE (confirmed by western blotting data using cell lines overexpressing truncated variants of apoE protein).
Date published: 2018-04-06
Rated 4 out of 5 by from Detection by Western BlotClean signal for endogenous ApoE from HepG2 cells as well as recombinant ApoE down to 5 ng.
Date published: 2018-04-05
Rated 5 out of 5 by from Wow !! Superb productOutstanding product for both western blot and IHC staining applications
Date published: 2017-02-04
Rated 5 out of 5 by from Reliable and specificThis antibody is great for Western blot detection of apoE, nice strong bands every time!
Date published: 2016-12-21
Rated 4 out of 5 by from Produced great immunoperoxidase cytoplasmicProduced great immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human testis tissue. -SCBT QC
Date published: 2015-05-12
Rated 5 out of 5 by from Works well for WB with Hep G2 whole cell lysateWorks well for WB with Hep G2 whole cell lysate. -SCBT QC
Date published: 2015-05-06
Rated 5 out of 5 by from Sharp signal obtained in WB with human plateletSharp signal obtained in WB with human platelet extract. -SCBT QC
Date published: 2015-03-10
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apolipoprotein E/apoE 抗体 (WU E-4) is rated 4.8 out of 5 by 8.
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