Date published: 2025-9-11
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apolipoprotein E/apoE Antibody (F-9) - Western Blotting - Image 376907
载脂蛋白 E 抗体 (F-9) HRP: sc-390925 HRP. Hep G2 (A), K-562 (B), EOC 20 (C), SK-N-MC (D) 和 Caki-1 (E) 全细胞裂解液中载脂蛋白表达的直接 Western 印迹分析.
载脂蛋白 E 抗体 (F-9) HRP: sc-390925 HRP. Hep G2 (A), K-562 (B), EOC 20 (C), SK-N-MC (D) 和 Caki-1 (E) 全细胞裂解液中载脂蛋白表达的直接 Western 印迹分析.

apolipoprotein E/apoE 抗体 (F-9): sc-390925

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说明书
  • apolipoprotein E/apoE抗体(F-9)是小鼠单克隆IgG2a κ, 在22篇文献中引用，规格为200 µg/ml
  • 免疫原氨基酸序列31-253位于human物种的apoE的内部区域内
  • 抗-apolipoprotein E/apoE 抗体 (F-9) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 apoE
  • 抗apolipoprotein E/apoE抗体（F-9）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取apolipoprotein E/apoE (F-9): sc-390925的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG2a BP-HRP 和 m-IgGκ BP-HRP 是 apolipoprotein E/apoE 抗体 (F-9) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与apolipoprotein E/apoE 抗体 (F-9) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 Apolipoprotein 抗体 包括 apoA-I, apoA-II, apoA-IV, apoA-V, apoC-I, apoC-II, apoC-III, apoC-IV, apoL1和apoL3

载脂蛋白E/apoE抗体（F-9）是一种IgG2a κ小鼠单克隆apoE抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、免疫组织化学（IHC，P）和酶联免疫吸附测定（ELISA）等方法检测小鼠、大鼠和人类来源的apoE。载脂蛋白E/apoE抗体（F-9）既可以非偶联形式的抗apoE抗体提供，也可以多种偶联形式的抗apoE抗体提供，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®偶联物。载脂蛋白E（apoE）是血浆脂蛋白的蛋白质组分，介导脂蛋白颗粒的结合、内化和分解代谢。它可以作为几种脂蛋白受体的配体，包括低密度脂蛋白（LDL，ApoB/E）受体和肝apoE（乳糜微粒残留物）受体。apoE在大多数器官中产生，并存在于所有血浆脂蛋白组分中，占极低密度脂蛋白（VLDL）的10-20%和高密度脂蛋白（HDL）的1-2%。在人类中已经描述了三种主要的apoE同工型（E2、E3和E4），它们之间仅相差一个或两个氨基酸。已经证明，雌激素受体可以通过ERa介导的途径上调apoE基因的表达，这表明apoE在动脉粥样硬化中可能具有潜在作用。这与小鼠的研究相一致，即提高血浆apoE水平可以保护小鼠免受饮食诱导的动脉粥样硬化。此外，apoE已被证明是增殖的强效抑制剂，因此可能在血管生成、肿瘤细胞生长和转移中发挥作用。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

apolipoprotein E/apoE 抗体 (F-9)

sc-390925
200 µg/ml
RMB2377.00

apolipoprotein E/apoE (F-9): m-IgGκ BP-HRP 套装

sc-523736
200 µg Ab, 40 µg BP
RMB2662.00

apolipoprotein E/apoE (F-9): m-IgG2a BP-HRP 套装

sc-546167
200 µg Ab; 10 µg BP
RMB2662.00

apolipoprotein E/apoE 抗体 (F-9) AC

sc-390925 AC
500 µg/ml, 25% agarose
RMB3129.00

apolipoprotein E/apoE 抗体 (F-9) HRP

sc-390925 HRP
200 µg/ml
RMB2377.00

apolipoprotein E/apoE 抗体 (F-9) FITC

sc-390925 FITC
200 µg/ml
RMB2482.00

apolipoprotein E/apoE 抗体 (F-9) PE

sc-390925 PE
200 µg/ml
RMB2580.00

apolipoprotein E/apoE 抗体 (F-9) Alexa Fluor® 488

sc-390925 AF488
200 µg/ml
RMB2685.00

apolipoprotein E/apoE 抗体 (F-9) Alexa Fluor® 546

sc-390925 AF546
200 µg/ml
RMB2685.00

apolipoprotein E/apoE 抗体 (F-9) Alexa Fluor® 594

sc-390925 AF594
200 µg/ml
RMB2685.00

apolipoprotein E/apoE 抗体 (F-9) Alexa Fluor® 647

sc-390925 AF647
200 µg/ml
RMB2685.00

apolipoprotein E/apoE 抗体 (F-9) Alexa Fluor® 680

sc-390925 AF680
200 µg/ml
RMB2685.00

apolipoprotein E/apoE 抗体 (F-9) Alexa Fluor® 790

sc-390925 AF790
200 µg/ml
RMB2685.00

apoE F-9 has replaced M-20. However, M-20 binds on the C-terminus of apoE, while F-9 binds within an internal region of apoE. Therefore, by F-9 we cannot detect apoE fragments previously detected with M-20. Any suggestion for another replacement?

Asked by: Aggelika
Thank you for your question. You can find all antibodies we offer against apoE by following this link: https://www.scbt.com/browse/apoe-Antik%C3%B6rper/_/N-pr9qxd?&_requestid=4836 apoE Antibody (A1.4): sc-13521, for instance, is specific against amino acids 126-191 of apoE of human origin.
Answered by: Tech Support Europe
Date published: 2019-11-22

Does this APOE antibody (F-9) have different affinity to different APOE isoforms (E2, E3, and E4)?

Asked by: CSYang
Thank you for your question. We have not performed any testing with this apoE Antibody (F-9): sc-390925 to determine reactivity with, or affinity for the different apoE isoforms, E2, E3 and E4.
Answered by: Technical Support
Date published: 2017-05-17

I need to use an antibody to detect the protein in samples of porcine origin. Do you know if this mouse monoclonal antibody will work?

Asked by: jerojero
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support 14
Date published: 2017-01-26

The epitope mapping does not make sense - this protein is 319 aa long if including the signaling peptide. Please could you clarify this epitope sequence?

Asked by: Jade
Thank you for your question, and for bringing this discrepancy to our attention. After reviewing the product, we have made a typo in the amino acid range listed as the immunogen for this antibody and incorrectly listed the range as 31-353. The correct range is 31-253. This antibody, apoE Antibody (F-9): sc-390925, is a mouse monoclonal antibody raised against a recombinant protein corresponding to amino acids 31-253 of apoE of human origin.
Answered by: Technical Support
Date published: 2023-03-23
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Rated 5 out of 5 by from Très bonne fonctionnalité sur plasma humainTrès bonne fonctionnalité. Donne un signal propre sur plasma humain.
Date published: 2022-05-23
Rated 4 out of 5 by from Good signal in WBIt works well in western blot for apoE detection in astrocyte conditioned medium. We used 1:200 dilution.
Date published: 2021-02-26
Rated 5 out of 5 by from Research associateIt works very well in western blotting, strong signal and low back grand..
Date published: 2018-11-29
Rated 5 out of 5 by from works for both mouse and human APOEsWe tried this APOE ab as a sample. The bands were pretty clear and no band in our KO mouse Brian samples or human ApoE KO lines.
Date published: 2018-04-05
Rated 5 out of 5 by from dilution 1:200Antibody can detect apoE by WB, the band is clear, antibody is very good
Date published: 2017-09-07
Rated 5 out of 5 by from very good antibodyClean WB blot with single strong band in human liver tissue extract and 293T whole cell lysate.
Date published: 2017-08-11
Rated 3 out of 5 by from apoEWB:there is a clear bands in mouse liver tissue,and used 1:500 dilution
Date published: 2017-08-09
Rated 4 out of 5 by from Works well for IF in fetal RPE cellsGood signal in differentiated fetal RPE cells by immunofluorescence.
Date published: 2017-04-20
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apolipoprotein E/apoE 抗体 (F-9) is rated 4.7 out of 5 by 12.
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